Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats
Background and purpose: Neural circuits and neurotransmitter systems within the basolateral amygdala (BLA) play roles in forming emotional memory through communication with the hippocampus. Therefore, in this study, the role of these neural circuits on synaptic plasticity was investigated by agonist...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-15363-en.html |
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| author | Seyedeh Kebria Noorani Vida Hojati Motahareh Rouhi Ardeshiri Esmaeil Akbari |
| author_facet | Seyedeh Kebria Noorani Vida Hojati Motahareh Rouhi Ardeshiri Esmaeil Akbari |
| author_sort | Seyedeh Kebria Noorani |
| collection | DOAJ |
| description | Background and purpose: Neural circuits and neurotransmitter systems within the basolateral amygdala (BLA) play roles in forming emotional memory through communication with the hippocampus. Therefore, in this study, the role of these neural circuits on synaptic plasticity was investigated by agonist injection of beta-adrenergic receptors into the BLA.
Materials and methods: In this experimental study, the electrophysiological recording from the hippocampus was investigated. Male Wistar rats (210-250 gr) were divided into two groups: control group that received saline (0.5 μl) and the treatment group that received clenbuterol (10 ng/0.5μl). Clenbuterol was also bilaterally injected into the BLA. Then, we studied the effect of clenbuterol on basal activity of the granular cells of the dentate gyrus (DG). Afterwards, a 200-Hz high-frequency stimulation protocol was applied to induce long-term potentiation (LTP). The amplitude of the population spike (PS) and the slope of the excitatory post synaptic potential (EPSP) were analyzed before and after the injection of clenbuterol.
Results: Injection of clenbuterol (10 ng) did not increase PS of the LTP compared to the control group in the DG region. Also, no significant increase was seen in the slope of EPSP in treatment group compared to the control group (P>0.05).
Conclusion: Clenbuterol administration into the basolateral amygdala (10 ng/0.5μl) did not affect basal electrical activity and synaptic reinforcement of the dentate granular cells in 90 minutes |
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| format | Article |
| id | doaj.art-b51d86931ca14f86815004a52b3a41d1 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-11T00:25:23Z |
| publishDate | 2021-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-b51d86931ca14f86815004a52b3a41d12023-01-08T08:43:29ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792021-01-0130192126132Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male RatsSeyedeh Kebria Noorani0Vida Hojati1Motahareh Rouhi Ardeshiri2Esmaeil Akbari3 PhD Student in Biology-Animal Physiology, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Assistant Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Assistant Professor, Department of Physiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Department of Physiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Neural circuits and neurotransmitter systems within the basolateral amygdala (BLA) play roles in forming emotional memory through communication with the hippocampus. Therefore, in this study, the role of these neural circuits on synaptic plasticity was investigated by agonist injection of beta-adrenergic receptors into the BLA. Materials and methods: In this experimental study, the electrophysiological recording from the hippocampus was investigated. Male Wistar rats (210-250 gr) were divided into two groups: control group that received saline (0.5 μl) and the treatment group that received clenbuterol (10 ng/0.5μl). Clenbuterol was also bilaterally injected into the BLA. Then, we studied the effect of clenbuterol on basal activity of the granular cells of the dentate gyrus (DG). Afterwards, a 200-Hz high-frequency stimulation protocol was applied to induce long-term potentiation (LTP). The amplitude of the population spike (PS) and the slope of the excitatory post synaptic potential (EPSP) were analyzed before and after the injection of clenbuterol. Results: Injection of clenbuterol (10 ng) did not increase PS of the LTP compared to the control group in the DG region. Also, no significant increase was seen in the slope of EPSP in treatment group compared to the control group (P>0.05). Conclusion: Clenbuterol administration into the basolateral amygdala (10 ng/0.5μl) did not affect basal electrical activity and synaptic reinforcement of the dentate granular cells in 90 minuteshttp://jmums.mazums.ac.ir/article-1-15363-en.htmlclenbuterolehippocampusbasolateral amygdalalong-term potentiation |
| spellingShingle | Seyedeh Kebria Noorani Vida Hojati Motahareh Rouhi Ardeshiri Esmaeil Akbari Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats Journal of Mazandaran University of Medical Sciences clenbuterole hippocampus basolateral amygdala long-term potentiation |
| title | Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats |
| title_full | Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats |
| title_fullStr | Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats |
| title_full_unstemmed | Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats |
| title_short | Effect of Clenbuterol Administration into the Basolateral Amygdala on Synaptic Plasticity in Dentate Gyrus Granule Cells in Male Rats |
| title_sort | effect of clenbuterol administration into the basolateral amygdala on synaptic plasticity in dentate gyrus granule cells in male rats |
| topic | clenbuterole hippocampus basolateral amygdala long-term potentiation |
| url | http://jmums.mazums.ac.ir/article-1-15363-en.html |
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