High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model
Abstract Although the relationships of cerebrovascular hemodynamic dysfunction with neurodegenerative diseases remain unclear, many studies have indicated that poor cerebral perfusion accelerates the progression of neurodegenerative diseases, such as Alzheimer's disease (AD). Small animal model...
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202302345 |
| _version_ | 1797389568608043008 |
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| author | Hsin Huang Pei‐Ling Hsu Sheng‐Feng Tsai Yi‐Hsiang Chuang De‐Quan Chen Guo‐Xuan Xu Chien Chen Yu‐Min Kuo Chih‐Chung Huang |
| author_facet | Hsin Huang Pei‐Ling Hsu Sheng‐Feng Tsai Yi‐Hsiang Chuang De‐Quan Chen Guo‐Xuan Xu Chien Chen Yu‐Min Kuo Chih‐Chung Huang |
| author_sort | Hsin Huang |
| collection | DOAJ |
| description | Abstract Although the relationships of cerebrovascular hemodynamic dysfunction with neurodegenerative diseases remain unclear, many studies have indicated that poor cerebral perfusion accelerates the progression of neurodegenerative diseases, such as Alzheimer's disease (AD). Small animal models are widely used in AD research. However, providing an imaging modality with a high spatiotemporal resolution and sufficiently large field of view to assess cerebrovascular hemodynamics in vivo remains a challenge. The present study proposes a novel technique for high‐spatiotemporal‐resolution vector micro‐Doppler imaging (HVμDI) based on contrast‐free ultrafast high frequency ultrasound imaging to visualize the cerebrovascular hemodynamics of the mouse, with a data acquisition time of 0.4 s, a minimal detectable vessel size of 38 µm, and a temporal resolution of 500 Hz. In vivo experiments are conducted on wild‐type and AD mice. Cerebrovascular hemodynamics are quantified using the cerebral vascular density, diameter, velocity, tortuosity, cortical flow pulsatility, and instant flow direction variations. Results reveal that AD significantly change the cerebrovascular hemodynamics. HVμDI offers new opportunities for in vivo analysis of cerebrovascular hemodynamics in neurodegenerative pathologies in preclinical animal research. |
| first_indexed | 2024-03-08T22:58:47Z |
| format | Article |
| id | doaj.art-b51eee2f218e4d59abb47ea8c5857044 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-08T22:58:47Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-b51eee2f218e4d59abb47ea8c58570442023-12-16T04:16:14ZengWileyAdvanced Science2198-38442023-12-011035n/an/a10.1002/advs.202302345High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice ModelHsin Huang0Pei‐Ling Hsu1Sheng‐Feng Tsai2Yi‐Hsiang Chuang3De‐Quan Chen4Guo‐Xuan Xu5Chien Chen6Yu‐Min Kuo7Chih‐Chung Huang8Department of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanDepartment of Anatomy School of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80708 TaiwanDepartment of Cell Biology and Anatomy College of Medicine National Cheng Kung University Tainan 70101 TaiwanDepartment of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanDepartment of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanDepartment of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanDepartment of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanDepartment of Cell Biology and Anatomy College of Medicine National Cheng Kung University Tainan 70101 TaiwanDepartment of Biomedical Engineering National Cheng Kung University Tainan 70101 TaiwanAbstract Although the relationships of cerebrovascular hemodynamic dysfunction with neurodegenerative diseases remain unclear, many studies have indicated that poor cerebral perfusion accelerates the progression of neurodegenerative diseases, such as Alzheimer's disease (AD). Small animal models are widely used in AD research. However, providing an imaging modality with a high spatiotemporal resolution and sufficiently large field of view to assess cerebrovascular hemodynamics in vivo remains a challenge. The present study proposes a novel technique for high‐spatiotemporal‐resolution vector micro‐Doppler imaging (HVμDI) based on contrast‐free ultrafast high frequency ultrasound imaging to visualize the cerebrovascular hemodynamics of the mouse, with a data acquisition time of 0.4 s, a minimal detectable vessel size of 38 µm, and a temporal resolution of 500 Hz. In vivo experiments are conducted on wild‐type and AD mice. Cerebrovascular hemodynamics are quantified using the cerebral vascular density, diameter, velocity, tortuosity, cortical flow pulsatility, and instant flow direction variations. Results reveal that AD significantly change the cerebrovascular hemodynamics. HVμDI offers new opportunities for in vivo analysis of cerebrovascular hemodynamics in neurodegenerative pathologies in preclinical animal research.https://doi.org/10.1002/advs.202302345Alzheimer's diseasecerebrovascular hemodynamicshigh‐frequency ultrasoundhigh‐resolution brain imagingvector flow imaging |
| spellingShingle | Hsin Huang Pei‐Ling Hsu Sheng‐Feng Tsai Yi‐Hsiang Chuang De‐Quan Chen Guo‐Xuan Xu Chien Chen Yu‐Min Kuo Chih‐Chung Huang High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model Advanced Science Alzheimer's disease cerebrovascular hemodynamics high‐frequency ultrasound high‐resolution brain imaging vector flow imaging |
| title | High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model |
| title_full | High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model |
| title_fullStr | High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model |
| title_full_unstemmed | High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model |
| title_short | High‐Spatiotemporal‐Resolution Ultrasound Flow Imaging to Determine Cerebrovascular Hemodynamics in Alzheimer's Disease Mice Model |
| title_sort | high spatiotemporal resolution ultrasound flow imaging to determine cerebrovascular hemodynamics in alzheimer s disease mice model |
| topic | Alzheimer's disease cerebrovascular hemodynamics high‐frequency ultrasound high‐resolution brain imaging vector flow imaging |
| url | https://doi.org/10.1002/advs.202302345 |
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