Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish

ABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury a...

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Main Authors: Soonil Koun, Hye-jin Park, Su-min Jung, Jin Joo Cha, Dae Ryong Cha, Young Sun Kang
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Animal Cells and Systems
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211
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author Soonil Koun
Hye-jin Park
Su-min Jung
Jin Joo Cha
Dae Ryong Cha
Young Sun Kang
author_facet Soonil Koun
Hye-jin Park
Su-min Jung
Jin Joo Cha
Dae Ryong Cha
Young Sun Kang
author_sort Soonil Koun
collection DOAJ
description ABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1–7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration.
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spelling doaj.art-b5214c4f71b1474aa4f132651da2c9c92024-01-08T17:12:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852023-12-0127111211910.1080/19768354.2023.2203211Puromycin-induced kidney injury and subsequent regeneration in adult zebrafishSoonil Koun0Hye-jin Park1Su-min Jung2Jin Joo Cha3Dae Ryong Cha4Young Sun Kang5Zebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaDepartment of Nephrology, Korea University Ansan Hospital, Ansan, Republic of KoreaDepartment of Nephrology, Korea University Ansan Hospital, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1–7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration.https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211Acute kidney injuryNephropathyToxicologyPuromycinRegeneration
spellingShingle Soonil Koun
Hye-jin Park
Su-min Jung
Jin Joo Cha
Dae Ryong Cha
Young Sun Kang
Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
Animal Cells and Systems
Acute kidney injury
Nephropathy
Toxicology
Puromycin
Regeneration
title Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
title_full Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
title_fullStr Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
title_full_unstemmed Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
title_short Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
title_sort puromycin induced kidney injury and subsequent regeneration in adult zebrafish
topic Acute kidney injury
Nephropathy
Toxicology
Puromycin
Regeneration
url https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211
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AT jinjoocha puromycininducedkidneyinjuryandsubsequentregenerationinadultzebrafish
AT daeryongcha puromycininducedkidneyinjuryandsubsequentregenerationinadultzebrafish
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