Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
ABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury a...
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Language: | English |
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Taylor & Francis Group
2023-12-01
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Series: | Animal Cells and Systems |
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Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211 |
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author | Soonil Koun Hye-jin Park Su-min Jung Jin Joo Cha Dae Ryong Cha Young Sun Kang |
author_facet | Soonil Koun Hye-jin Park Su-min Jung Jin Joo Cha Dae Ryong Cha Young Sun Kang |
author_sort | Soonil Koun |
collection | DOAJ |
description | ABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1–7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration. |
first_indexed | 2024-03-08T15:57:52Z |
format | Article |
id | doaj.art-b5214c4f71b1474aa4f132651da2c9c9 |
institution | Directory Open Access Journal |
issn | 1976-8354 2151-2485 |
language | English |
last_indexed | 2024-03-08T15:57:52Z |
publishDate | 2023-12-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | Animal Cells and Systems |
spelling | doaj.art-b5214c4f71b1474aa4f132651da2c9c92024-01-08T17:12:29ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852023-12-0127111211910.1080/19768354.2023.2203211Puromycin-induced kidney injury and subsequent regeneration in adult zebrafishSoonil Koun0Hye-jin Park1Su-min Jung2Jin Joo Cha3Dae Ryong Cha4Young Sun Kang5Zebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaDepartment of Nephrology, Korea University Ansan Hospital, Ansan, Republic of KoreaDepartment of Nephrology, Korea University Ansan Hospital, Ansan, Republic of KoreaZebrafish Translational Medical Research Center, Korea University, Ansan, Republic of KoreaABSTRACTPuromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear. In this work, we examined the characteristics of kidney injury and subsequent regeneration following puromycin treatment in adult zebrafish. We intraperitoneally injected 100 μg of puromycin into zebrafish; sacrificed them at 1, 3, 5, 7, or 14 days post-injection (dpi); and examined the morphological, functional, and molecular changes in the kidney. Puromycin-treated zebrafish presented more rapid clearance of rhodamine dextran than control animals. Morphological changes were observed immediately after the puromycin injection (1–7 dpi) and had recovered by 14 dpi. The mRNA production of lhx1a, a renal progenitor marker, increased during recovery from kidney injury. Levels of NFκB, TNFα, Nampt, and p-ERK increased significantly during nephron injury and regeneration, and Sirt1, FOXO1, pax2, and wt1b showed an increasing tendency. However, TGF-β1 and smad5 production did not show any changes after puromycin treatment. This study provides evidence that puromycin-induced injury in adult zebrafish kidneys is a potential tool for evaluating the mechanism of nephron injury and subsequent regeneration.https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211Acute kidney injuryNephropathyToxicologyPuromycinRegeneration |
spellingShingle | Soonil Koun Hye-jin Park Su-min Jung Jin Joo Cha Dae Ryong Cha Young Sun Kang Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish Animal Cells and Systems Acute kidney injury Nephropathy Toxicology Puromycin Regeneration |
title | Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish |
title_full | Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish |
title_fullStr | Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish |
title_full_unstemmed | Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish |
title_short | Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish |
title_sort | puromycin induced kidney injury and subsequent regeneration in adult zebrafish |
topic | Acute kidney injury Nephropathy Toxicology Puromycin Regeneration |
url | https://www.tandfonline.com/doi/10.1080/19768354.2023.2203211 |
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