Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L
Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transporte...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-10-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-2065-7 |
| _version_ | 1818335840453001216 |
|---|---|
| author | Jianfang Gao Li Shen Jingli Yuan Hongli Zheng Quansheng Su Weiguang Yang Liqing Zhang Vitalis Ekene Nnaemeka Jie Sun Liping Ke Yuqiang Sun |
| author_facet | Jianfang Gao Li Shen Jingli Yuan Hongli Zheng Quansheng Su Weiguang Yang Liqing Zhang Vitalis Ekene Nnaemeka Jie Sun Liping Ke Yuqiang Sun |
| author_sort | Jianfang Gao |
| collection | DOAJ |
| description | Abstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cells after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS, GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi, GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS, GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi, GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS, GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering. |
| first_indexed | 2024-12-13T14:29:49Z |
| format | Article |
| id | doaj.art-b5269688b8514968a7e2ffd20c4d7fd3 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-13T14:29:49Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-b5269688b8514968a7e2ffd20c4d7fd32022-12-21T23:41:52ZengBMCBMC Plant Biology1471-22292019-10-0119111810.1186/s12870-019-2065-7Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum LJianfang Gao0Li Shen1Jingli Yuan2Hongli Zheng3Quansheng Su4Weiguang Yang5Liqing Zhang6Vitalis Ekene Nnaemeka7Jie Sun8Liping Ke9Yuqiang Sun10Plant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityCollege of Agriculture/The Key Laboratory of Oasis Eco-Agriculture, Shihezi University, Shihezi 832000Plant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityPlant Genomics & Molecular Improvement of Colored Fiber Lab, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityAbstract Background The formation of natural colored fibers mainly results from the accumulation of different anthocyanidins and their derivatives in the fibers of Gossypium hirsutum L. Chalcone synthase (CHS) is the first committed enzyme of flavonoid biosynthesis, and anthocyanidins are transported into fiber cells after biosynthesis mainly by Anthocyanidin reductase (ANR) and Leucoanthocyanidin reductase (LAR) to present diverse colors with distinct stability. The biochemical and molecular mechanism of pigment formation in natural colored cotton fiber is not clear. Results The three key genes of GhCHS, GhANR and GhLAR were predominantly expressed in the developing fibers of colored cotton. In the GhCHSi, GhANRi and GhLARi transgenic cottons, the expression levels of GhCHS, GhANR and GhLAR significantly decreased in the developing cotton fiber, negatively correlated with the content of anthocyanidins and the color depth of cotton fiber. In colored cotton Zongxu1 (ZX1) and the GhCHSi, GhANRi and GhLARi transgenic lines of ZX1, HZ and ZH, the anthocyanidin contents of the leaves, cotton kernels, the mixture of fiber and seedcoat were all changed and positively correlated with the fiber color. Conclusion The three genes of GhCHS, GhANR and GhLAR were predominantly expressed early in developing colored cotton fibers and identified to be a key genes of cotton fiber color formation. The expression levels of the three genes affected the anthocyanidin contents and fiber color depth. So the three genes played a crucial part in cotton fiber color formation and has important significant to improve natural colored cotton quality and create new colored cotton germplasm resources by genetic engineering.http://link.springer.com/article/10.1186/s12870-019-2065-7G. hirsutum L.Fiber colorAnthocyanidinGhCHSGhANRGhLAR |
| spellingShingle | Jianfang Gao Li Shen Jingli Yuan Hongli Zheng Quansheng Su Weiguang Yang Liqing Zhang Vitalis Ekene Nnaemeka Jie Sun Liping Ke Yuqiang Sun Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L BMC Plant Biology G. hirsutum L. Fiber color Anthocyanidin GhCHS GhANR GhLAR |
| title | Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L |
| title_full | Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L |
| title_fullStr | Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L |
| title_full_unstemmed | Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L |
| title_short | Functional analysis of GhCHS, GhANR and GhLAR in colored fiber formation of Gossypium hirsutum L |
| title_sort | functional analysis of ghchs ghanr and ghlar in colored fiber formation of gossypium hirsutum l |
| topic | G. hirsutum L. Fiber color Anthocyanidin GhCHS GhANR GhLAR |
| url | http://link.springer.com/article/10.1186/s12870-019-2065-7 |
| work_keys_str_mv | AT jianfanggao functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT lishen functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT jingliyuan functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT honglizheng functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT quanshengsu functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT weiguangyang functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT liqingzhang functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT vitalisekenennaemeka functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT jiesun functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT lipingke functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml AT yuqiangsun functionalanalysisofghchsghanrandghlarincoloredfiberformationofgossypiumhirsutuml |