Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation
Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using cal...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2021-06-01
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| Series: | Indonesian Journal of Pharmacy |
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| Online Access: | https://jurnal.ugm.ac.id/v3/IJP/article/view/1062 |
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| author | Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati |
| author_facet | Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati |
| author_sort | Woro Anindito Sri Tunjung |
| collection | DOAJ |
| description | Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus. |
| first_indexed | 2024-12-20T03:09:01Z |
| format | Article |
| id | doaj.art-b5272b6a9a1e437ab01725575091cb22 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-20T03:09:01Z |
| publishDate | 2021-06-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-b5272b6a9a1e437ab01725575091cb222022-12-21T19:55:31ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862021-06-0117919210.22146/ijp.10621062Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post PreservationWoro Anindito Sri Tunjung0Sudewi Fajarina1Beni Hendro Prabowo2Frisca Damayanti3Asti Widyasari4Aries Bagus Sasongko5Ari Indrianto6Endang Semiarti7Lisna Hidayati8Lab. of Biochemistry Faculty of Biology Universitas Gadjah MadaDe Britto College Senior High School YogyakartaBiology Education Department, Faculty of Teacher Training and Education, Tidar University Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of SciencesUndergraduate Program, Faculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaPrevious studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.https://jurnal.ugm.ac.id/v3/IJP/article/view/1062kaffir lime callus, preservation, cytotoxicity, bioactive compounds |
| spellingShingle | Woro Anindito Sri Tunjung Sudewi Fajarina Beni Hendro Prabowo Frisca Damayanti Asti Widyasari Aries Bagus Sasongko Ari Indrianto Endang Semiarti Lisna Hidayati Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation Indonesian Journal of Pharmacy kaffir lime callus, preservation, cytotoxicity, bioactive compounds |
| title | Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation |
| title_full | Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation |
| title_fullStr | Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation |
| title_full_unstemmed | Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation |
| title_short | Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation |
| title_sort | evaluation of anticancer bioactive compounds and cytotoxicity of kaffir lime citrus hystrix dc callus extract post preservation |
| topic | kaffir lime callus, preservation, cytotoxicity, bioactive compounds |
| url | https://jurnal.ugm.ac.id/v3/IJP/article/view/1062 |
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