Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk
Totally 75 raw milk samples were analysed with the methods employing the media compared - MYPA, PEMBA, BrillianceTM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seems to be the most suitable for dairy plants laboratories because there is only low risk of mistaken i...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-12-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0011_methods-for-detection-of-bacillus-sp-b-cereus-and-b-licheniformis-in-raw-milk.php |
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| author | Irena NĚMEČKOVÁ Kateřina SOLICHOVÁ Petr ROUBAL Barbora UHROVÁ Eva ŠVIRÁKOVÁ |
| author_facet | Irena NĚMEČKOVÁ Kateřina SOLICHOVÁ Petr ROUBAL Barbora UHROVÁ Eva ŠVIRÁKOVÁ |
| author_sort | Irena NĚMEČKOVÁ |
| collection | DOAJ |
| description | Totally 75 raw milk samples were analysed with the methods employing the media compared - MYPA, PEMBA, BrillianceTM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seems to be the most suitable for dairy plants laboratories because there is only low risk of mistaken identity. However, the samples containing miscellaneous micro-flora should be heat-inactivated before plating. Both positive and negative strains (totally 132) were isolated. Twelve strains, which could cause problems in the evaluation of the plates, were selected and identified by phenotyping and by PCR methods for Bacillus sp., B. cereus, and B. licheniformis. The PCR methods differed in their selectivity within particular bacilli group, within genera Bacillus, and within raw milk microflora. |
| first_indexed | 2024-04-10T08:35:09Z |
| format | Article |
| id | doaj.art-b528c38e86d945a88101184d22db09a2 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:09Z |
| publishDate | 2011-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-b528c38e86d945a88101184d22db09a22023-02-23T03:27:40ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-12-0129Special IssueS55S6010.17221/313/2011-CJFScjf-201110-0011Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milkIrena NĚMEČKOVÁ0Kateřina SOLICHOVÁ1Petr ROUBAL2Barbora UHROVÁ3Eva ŠVIRÁKOVÁ4Dairy Research Institute Ltd., Prague, Czech RepublicDepartment of Dairy and Fat Technology, Institute of Chemical Technology, Prague, Czech RepublicMILCOM Company Limited, Prague, Czech RepublicMILCOM Company Limited, Prague, Czech RepublicDepartment of Dairy and Fat Technology, Institute of Chemical Technology, Prague, Czech RepublicTotally 75 raw milk samples were analysed with the methods employing the media compared - MYPA, PEMBA, BrillianceTM Bacillus cereus agar, and HiCrome Bacillus agar. The reference method with MYPA seems to be the most suitable for dairy plants laboratories because there is only low risk of mistaken identity. However, the samples containing miscellaneous micro-flora should be heat-inactivated before plating. Both positive and negative strains (totally 132) were isolated. Twelve strains, which could cause problems in the evaluation of the plates, were selected and identified by phenotyping and by PCR methods for Bacillus sp., B. cereus, and B. licheniformis. The PCR methods differed in their selectivity within particular bacilli group, within genera Bacillus, and within raw milk microflora.https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0011_methods-for-detection-of-bacillus-sp-b-cereus-and-b-licheniformis-in-raw-milk.phpcultivation methodchromogenic mediumgenera-specific pcrspecies-specific pcr |
| spellingShingle | Irena NĚMEČKOVÁ Kateřina SOLICHOVÁ Petr ROUBAL Barbora UHROVÁ Eva ŠVIRÁKOVÁ Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk Czech Journal of Food Sciences cultivation method chromogenic medium genera-specific pcr species-specific pcr |
| title | Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk |
| title_full | Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk |
| title_fullStr | Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk |
| title_full_unstemmed | Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk |
| title_short | Methods for detection of Bacillus sp., B. cereus, and B. licheniformis in raw milk |
| title_sort | methods for detection of bacillus sp b cereus and b licheniformis in raw milk |
| topic | cultivation method chromogenic medium genera-specific pcr species-specific pcr |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0011_methods-for-detection-of-bacillus-sp-b-cereus-and-b-licheniformis-in-raw-milk.php |
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