In vitro propagation of bastard balm (Melittis melissophyllum L.)
An efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containin...
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| Format: | Article |
| Language: | English |
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Polish Ministry of Science and Higher Education
2015-09-01
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| Series: | Herba Polonica |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/hepo-2015-0023 |
| _version_ | 1797760375046799360 |
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| author | Bączek Katarzyna Szymborska-Sandhu Izabela Pawełczak Anna Węglarz Zenon |
| author_facet | Bączek Katarzyna Szymborska-Sandhu Izabela Pawełczak Anna Węglarz Zenon |
| author_sort | Bączek Katarzyna |
| collection | DOAJ |
| description | An efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in ex vitro conditions very well. |
| first_indexed | 2024-03-12T18:57:42Z |
| format | Article |
| id | doaj.art-b52965e9f2864f2fb353a5dbaaac6072 |
| institution | Directory Open Access Journal |
| issn | 2449-8343 |
| language | English |
| last_indexed | 2024-03-12T18:57:42Z |
| publishDate | 2015-09-01 |
| publisher | Polish Ministry of Science and Higher Education |
| record_format | Article |
| series | Herba Polonica |
| spelling | doaj.art-b52965e9f2864f2fb353a5dbaaac60722023-08-02T06:45:28ZengPolish Ministry of Science and Higher EducationHerba Polonica2449-83432015-09-01613677610.1515/hepo-2015-0023hepo-2015-0023In vitro propagation of bastard balm (Melittis melissophyllum L.)Bączek Katarzyna0Szymborska-Sandhu Izabela1Pawełczak Anna2Węglarz Zenon3Laboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Nowoursynowska 166 02-787 Warsaw, PolandLaboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Nowoursynowska 166 02-787 Warsaw, PolandLaboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Nowoursynowska 166 02-787 Warsaw, PolandLaboratory of New Herbal Products, Department of Vegetable and Medicinal Plants, Warsaw University of Life Sciences – SGGW, Nowoursynowska 166 02-787 Warsaw, PolandAn efficient method for in vitro propagation of bastard balm by enhanced axillary shoot branching has been developed. The material to establish in vitro culture was shoot tips collected from three-year-old plants in May. The shoots obtained from initial explants were placed on MS/B5 medium containing 0.1, 0.5 or 1.0 mg/l BA with 0.01 mg/l NAA or without the auxin. The highest number of shoots per explant was obtained on the medium with 1.0 mg/l BA (3.9 shoots per explant). For the rooting of shoots ½ MS/B5 with IBA (0.25, 0.50 and 1.0 mg/l) medium was used. The medium without plant growth regulators served as a control. The best root regeneration was observed on the medium without IBA (87.1% of cuttings rooted). IBA used in the medium for shoot rooting affected the morphological traits of obtained microcuttings but not affected their weight. Irrespective of auxin concentration in this medium, obtained microcuttings acclimated in ex vitro conditions very well.https://doi.org/10.1515/hepo-2015-0023in vitroaxillary shootsms/b5 mediumnaabaibaex vitro adaptationin vitropędy bocznepożywka ms/b5naabaibaadaptacja ex vitro |
| spellingShingle | Bączek Katarzyna Szymborska-Sandhu Izabela Pawełczak Anna Węglarz Zenon In vitro propagation of bastard balm (Melittis melissophyllum L.) Herba Polonica in vitro axillary shoots ms/b5 medium naa ba iba ex vitro adaptation in vitro pędy boczne pożywka ms/b5 naa ba iba adaptacja ex vitro |
| title | In vitro propagation of bastard balm (Melittis melissophyllum L.) |
| title_full | In vitro propagation of bastard balm (Melittis melissophyllum L.) |
| title_fullStr | In vitro propagation of bastard balm (Melittis melissophyllum L.) |
| title_full_unstemmed | In vitro propagation of bastard balm (Melittis melissophyllum L.) |
| title_short | In vitro propagation of bastard balm (Melittis melissophyllum L.) |
| title_sort | in vitro propagation of bastard balm melittis melissophyllum l |
| topic | in vitro axillary shoots ms/b5 medium naa ba iba ex vitro adaptation in vitro pędy boczne pożywka ms/b5 naa ba iba adaptacja ex vitro |
| url | https://doi.org/10.1515/hepo-2015-0023 |
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