Cold atmospheric plasma enhances osteoblast differentiation.
This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5500351?pdf=render |
| _version_ | 1818478345853075456 |
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| author | Kanako Tominami Hiroyasu Kanetaka Shota Sasaki Takayuki Mokudai Toshiro Kaneko Yoshimi Niwano |
| author_facet | Kanako Tominami Hiroyasu Kanetaka Shota Sasaki Takayuki Mokudai Toshiro Kaneko Yoshimi Niwano |
| author_sort | Kanako Tominami |
| collection | DOAJ |
| description | This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration. |
| first_indexed | 2024-12-10T09:46:54Z |
| format | Article |
| id | doaj.art-b52ad8da9e2342d2b94807342b2ca70a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:46:54Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b52ad8da9e2342d2b94807342b2ca70a2022-12-22T01:53:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018050710.1371/journal.pone.0180507Cold atmospheric plasma enhances osteoblast differentiation.Kanako TominamiHiroyasu KanetakaShota SasakiTakayuki MokudaiToshiro KanekoYoshimi NiwanoThis study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration.http://europepmc.org/articles/PMC5500351?pdf=render |
| spellingShingle | Kanako Tominami Hiroyasu Kanetaka Shota Sasaki Takayuki Mokudai Toshiro Kaneko Yoshimi Niwano Cold atmospheric plasma enhances osteoblast differentiation. PLoS ONE |
| title | Cold atmospheric plasma enhances osteoblast differentiation. |
| title_full | Cold atmospheric plasma enhances osteoblast differentiation. |
| title_fullStr | Cold atmospheric plasma enhances osteoblast differentiation. |
| title_full_unstemmed | Cold atmospheric plasma enhances osteoblast differentiation. |
| title_short | Cold atmospheric plasma enhances osteoblast differentiation. |
| title_sort | cold atmospheric plasma enhances osteoblast differentiation |
| url | http://europepmc.org/articles/PMC5500351?pdf=render |
| work_keys_str_mv | AT kanakotominami coldatmosphericplasmaenhancesosteoblastdifferentiation AT hiroyasukanetaka coldatmosphericplasmaenhancesosteoblastdifferentiation AT shotasasaki coldatmosphericplasmaenhancesosteoblastdifferentiation AT takayukimokudai coldatmosphericplasmaenhancesosteoblastdifferentiation AT toshirokaneko coldatmosphericplasmaenhancesosteoblastdifferentiation AT yoshiminiwano coldatmosphericplasmaenhancesosteoblastdifferentiation |