Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts
Polyphenols and flavonoids possess a variety of biological activities including antioxidant and anti-tumor activities. Ixora parviflora is a member of the flavonoid-rich Rubiaceae family of flowering plants and used as folk medicine in India. The aim of this study was to investigate the antioxidant...
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MDPI AG
2011-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/7/5735/ |
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| author | Hsiu-Mei Chiang Jung-Hsiang Chang Shih-Mei Wu Chien-Wen Chen Hua-Hsien Chiu Pei-Ching Fan Kuo-Ching Wen |
| author_facet | Hsiu-Mei Chiang Jung-Hsiang Chang Shih-Mei Wu Chien-Wen Chen Hua-Hsien Chiu Pei-Ching Fan Kuo-Ching Wen |
| author_sort | Hsiu-Mei Chiang |
| collection | DOAJ |
| description | Polyphenols and flavonoids possess a variety of biological activities including antioxidant and anti-tumor activities. Ixora parviflora is a member of the flavonoid-rich Rubiaceae family of flowering plants and used as folk medicine in India. The aim of this study was to investigate the antioxidant activity of Ixora parviflora extract (IPE) in a cell-free system and erythrocytes, and the ability of IPE to inhibit reactive oxygen species (ROS) generation in human fibroblasts (Hs68) after ultraviolet (UV) exposure. Various in vitro antioxidant assays were employed in this study. The extraction yield of IPE was 17.4 ± 3.9%, the total phenolic content of IPE was 26.2 μg gallic acid equivalent (GAE)/mg leaves dry weight and the total flavonoids content was 54.2 ± 4.4 μg quercetin equvalent (QE)/mg extract. The content of chlorogenic acid was 9.7 ± 1.2 mg/g extract. IPE at 1000 μg/mL exhibited a reducing capacity of 90.5 ± 0.6%, a 1,1-diphenyl-2-picrylhydrazy (DPPH) radical scavenging activity of 96.0 ± 0.4%, a ferrous chelating activity of 72.2 ± 3.5%, a hydroxyl radical scavenging activity of 96.8 ± 1.4%, and a hydrogen peroxide scavenging activity of 99.5 ± 3.3%. IPE at 500 μg/mL also possessed inhibitory activity against 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced hemolysis of erythrocytes (89.4 ± 1.8%) and resulted in a 52.9% reduction in ROS generation in UV-exposed fibroblasts. According to our findings, IPE is a potent antioxidant and a potential anti-photoaging agent. |
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| format | Article |
| id | doaj.art-b52b41939f6148c1a6a736e4face9136 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T13:57:27Z |
| publishDate | 2011-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b52b41939f6148c1a6a736e4face91362022-12-22T01:45:54ZengMDPI AGMolecules1420-30492011-07-011675735575210.3390/molecules16075735Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human FibroblastsHsiu-Mei ChiangJung-Hsiang ChangShih-Mei WuChien-Wen ChenHua-Hsien ChiuPei-Ching FanKuo-Ching WenPolyphenols and flavonoids possess a variety of biological activities including antioxidant and anti-tumor activities. Ixora parviflora is a member of the flavonoid-rich Rubiaceae family of flowering plants and used as folk medicine in India. The aim of this study was to investigate the antioxidant activity of Ixora parviflora extract (IPE) in a cell-free system and erythrocytes, and the ability of IPE to inhibit reactive oxygen species (ROS) generation in human fibroblasts (Hs68) after ultraviolet (UV) exposure. Various in vitro antioxidant assays were employed in this study. The extraction yield of IPE was 17.4 ± 3.9%, the total phenolic content of IPE was 26.2 μg gallic acid equivalent (GAE)/mg leaves dry weight and the total flavonoids content was 54.2 ± 4.4 μg quercetin equvalent (QE)/mg extract. The content of chlorogenic acid was 9.7 ± 1.2 mg/g extract. IPE at 1000 μg/mL exhibited a reducing capacity of 90.5 ± 0.6%, a 1,1-diphenyl-2-picrylhydrazy (DPPH) radical scavenging activity of 96.0 ± 0.4%, a ferrous chelating activity of 72.2 ± 3.5%, a hydroxyl radical scavenging activity of 96.8 ± 1.4%, and a hydrogen peroxide scavenging activity of 99.5 ± 3.3%. IPE at 500 μg/mL also possessed inhibitory activity against 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced hemolysis of erythrocytes (89.4 ± 1.8%) and resulted in a 52.9% reduction in ROS generation in UV-exposed fibroblasts. According to our findings, IPE is a potent antioxidant and a potential anti-photoaging agent.http://www.mdpi.com/1420-3049/16/7/5735/Ixora parvifloraantioxidantmetal chelatingreducing powerradical scavengingreactive oxygen species |
| spellingShingle | Hsiu-Mei Chiang Jung-Hsiang Chang Shih-Mei Wu Chien-Wen Chen Hua-Hsien Chiu Pei-Ching Fan Kuo-Ching Wen Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts Molecules Ixora parviflora antioxidant metal chelating reducing power radical scavenging reactive oxygen species |
| title | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts |
| title_full | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts |
| title_fullStr | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts |
| title_full_unstemmed | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts |
| title_short | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts |
| title_sort | antioxidant activity of ixora parviflora in a cell cell free system and in uv exposed human fibroblasts |
| topic | Ixora parviflora antioxidant metal chelating reducing power radical scavenging reactive oxygen species |
| url | http://www.mdpi.com/1420-3049/16/7/5735/ |
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