Infrequent p53 gene alterations in ulcerative colitis
The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1999-09-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005 |
| _version_ | 1811193249802485760 |
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| author | R. Mattar A.M. Alexandrino A.A. Laudanna |
| author_facet | R. Mattar A.M. Alexandrino A.A. Laudanna |
| author_sort | R. Mattar |
| collection | DOAJ |
| description | The purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at the p53 intron 6 was high, 11 out of 12 (92%), whereas no LOH was observed. LOH affecting p53 exon 4 was not detected in lesions from 5 of 12 patients (42%). In ulcerative colitis, tumor progression is similar to that in sporadic colon cancer, and other oncogenes and tumor suppressor genes are likely to be mutated before the p53 gene. |
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| format | Article |
| id | doaj.art-b52d7effbbfd4748aeff8057ad05e0a2 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T00:05:15Z |
| publishDate | 1999-09-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-b52d7effbbfd4748aeff8057ad05e0a22022-12-22T03:56:08ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1999-09-013291083108810.1590/S0100-879X1999000900005Infrequent p53 gene alterations in ulcerative colitisR. MattarA.M. AlexandrinoA.A. LaudannaThe purpose of this study was to determine whether point mutations and loss of the p53 gene take place in ulcerative colitis which is histologically negative for dysplasia. DNA was extracted from 13 frozen rectal or colon biopsies and blood samples. Ulcerative colitis was classified histologically as active (10 cases) and inactive (3 cases). Exons 5-8 were amplified by PCR, treated with exonuclease and shrimp alkaline phosphatase and sequenced by the dideoxy chain termination method with the Sequenase Version 2.0 DNA sequencing kit. PCR products of intron 6 and exon 4 were digested with MspI and AccII, respectively, for RFLP analysis. No p53 gene mutation was detected in these cases. The number of informative patients for loss of heterozygosity (LOH) at the p53 intron 6 was high, 11 out of 12 (92%), whereas no LOH was observed. LOH affecting p53 exon 4 was not detected in lesions from 5 of 12 patients (42%). In ulcerative colitis, tumor progression is similar to that in sporadic colon cancer, and other oncogenes and tumor suppressor genes are likely to be mutated before the p53 gene.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005p53 geneulcerative colitisLOHmutation |
| spellingShingle | R. Mattar A.M. Alexandrino A.A. Laudanna Infrequent p53 gene alterations in ulcerative colitis Brazilian Journal of Medical and Biological Research p53 gene ulcerative colitis LOH mutation |
| title | Infrequent p53 gene alterations in ulcerative colitis |
| title_full | Infrequent p53 gene alterations in ulcerative colitis |
| title_fullStr | Infrequent p53 gene alterations in ulcerative colitis |
| title_full_unstemmed | Infrequent p53 gene alterations in ulcerative colitis |
| title_short | Infrequent p53 gene alterations in ulcerative colitis |
| title_sort | infrequent p53 gene alterations in ulcerative colitis |
| topic | p53 gene ulcerative colitis LOH mutation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900005 |
| work_keys_str_mv | AT rmattar infrequentp53genealterationsinulcerativecolitis AT amalexandrino infrequentp53genealterationsinulcerativecolitis AT aalaudanna infrequentp53genealterationsinulcerativecolitis |