Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo
In vivo fluorescent imaging technique is a strong tool to visualize the various cellularevents such as the proliferation, differentiation, migration, and a lineage tracing inliving cells requiring no further experimental procedure such as immunostaining. Someunique and dynamic histone exchanges occu...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-07-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2014.00030/full |
| _version_ | 1811243268378198016 |
|---|---|
| author | Yoshinori eMakino Erina eInoue Masashi eHada Keisuke eAoshima Satsuki eKitano Hitoshi eMiyachi Yuki eOkada |
| author_facet | Yoshinori eMakino Erina eInoue Masashi eHada Keisuke eAoshima Satsuki eKitano Hitoshi eMiyachi Yuki eOkada |
| author_sort | Yoshinori eMakino |
| collection | DOAJ |
| description | In vivo fluorescent imaging technique is a strong tool to visualize the various cellularevents such as the proliferation, differentiation, migration, and a lineage tracing inliving cells requiring no further experimental procedure such as immunostaining. Someunique and dynamic histone exchanges occur. Since the timing and types of histoneexchanges defines the particular stages of spermatogenesis, visualizing certain types ofhistones in testes is useful not only for researching specific histone dynamics, but alsofor monitoring the stages of spermatogenesis in vivo. In this study, we report theestablishment of two transgenic (Tg) mouse lines expressing histone H4-Venus (H4V)and histone H3.3-mCherry (H33C) fusion proteins in testicular germ cells, anddemonstrated their utility for monitoring germ cell development in vivo. Because of thechoice of promoter as well as the nature of these histones, H4V and H33C wereexclusively expressed in the germ cells of the distinct stages, which allowed thedetermination of spermatogenic stages in real time. In addition, disappearance of H4Vand H33C at particular stages of differentiation/fertilization also represented dynamichistone removal. Collectively, these Tg mice are a valuable resource not only formonitoring differentiation stages, but also for studying the chromatin dynamics ofpost-natal testicular germ cell development in vivo. |
| first_indexed | 2024-04-12T14:04:27Z |
| format | Article |
| id | doaj.art-b52ecb1c16434f3aafad7936d9b2b220 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-12T14:04:27Z |
| publishDate | 2014-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-b52ecb1c16434f3aafad7936d9b2b2202022-12-22T03:30:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2014-07-01210.3389/fcell.2014.00030102318Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivoYoshinori eMakino0Erina eInoue1Masashi eHada2Keisuke eAoshima3Satsuki eKitano4Hitoshi eMiyachi5Yuki eOkada6The University of TokyoThe University of TokyoThe University of TokyoHokkaido UniversityKyoto UniversityKyoto UniversityThe University of TokyoIn vivo fluorescent imaging technique is a strong tool to visualize the various cellularevents such as the proliferation, differentiation, migration, and a lineage tracing inliving cells requiring no further experimental procedure such as immunostaining. Someunique and dynamic histone exchanges occur. Since the timing and types of histoneexchanges defines the particular stages of spermatogenesis, visualizing certain types ofhistones in testes is useful not only for researching specific histone dynamics, but alsofor monitoring the stages of spermatogenesis in vivo. In this study, we report theestablishment of two transgenic (Tg) mouse lines expressing histone H4-Venus (H4V)and histone H3.3-mCherry (H33C) fusion proteins in testicular germ cells, anddemonstrated their utility for monitoring germ cell development in vivo. Because of thechoice of promoter as well as the nature of these histones, H4V and H33C wereexclusively expressed in the germ cells of the distinct stages, which allowed thedetermination of spermatogenic stages in real time. In addition, disappearance of H4Vand H33C at particular stages of differentiation/fertilization also represented dynamichistone removal. Collectively, these Tg mice are a valuable resource not only formonitoring differentiation stages, but also for studying the chromatin dynamics ofpost-natal testicular germ cell development in vivo.http://journal.frontiersin.org/Journal/10.3389/fcell.2014.00030/fullHistonesSpermatogenesisTransplantation, Homologousspermatogonial stem cellsreporter mice |
| spellingShingle | Yoshinori eMakino Erina eInoue Masashi eHada Keisuke eAoshima Satsuki eKitano Hitoshi eMiyachi Yuki eOkada Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo Frontiers in Cell and Developmental Biology Histones Spermatogenesis Transplantation, Homologous spermatogonial stem cells reporter mice |
| title | Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo |
| title_full | Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo |
| title_fullStr | Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo |
| title_full_unstemmed | Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo |
| title_short | Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo |
| title_sort | generation of a dual color reporter mouse line to monitor spermatogenesis in vivo |
| topic | Histones Spermatogenesis Transplantation, Homologous spermatogonial stem cells reporter mice |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2014.00030/full |
| work_keys_str_mv | AT yoshinoriemakino generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT erinaeinoue generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT masashiehada generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT keisukeeaoshima generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT satsukiekitano generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT hitoshiemiyachi generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo AT yukieokada generationofadualcolorreportermouselinetomonitorspermatogenesisinvivo |