MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS
The aim of the study was to determine the degree of contamination of three kinds of female mosquitoes (Culex, Aedes, Anopheles) in some areas of Moscow and Nizhny Novgorod regions at different times of the warm season of the year (from May to September). Examined adult female mosquitoes collected in...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2022-12-01
|
| Series: | Сеченовский вестник |
| Subjects: | |
| Online Access: | https://www.sechenovmedj.com/jour/article/view/822 |
| _version_ | 1797873602118287360 |
|---|---|
| author | V. M. Rakova |
| author_facet | V. M. Rakova |
| author_sort | V. M. Rakova |
| collection | DOAJ |
| description | The aim of the study was to determine the degree of contamination of three kinds of female mosquitoes (Culex, Aedes, Anopheles) in some areas of Moscow and Nizhny Novgorod regions at different times of the warm season of the year (from May to September). Examined adult female mosquitoes collected in the Moscow and Nizhny Novgorod region in the summer of 2010 as potential vectors of Dirofilaria immitis and Dirofilaria repens. The presence of Dirofilaria spp. was detected by polymerase chain reaction using species-specific primers for D. immitis and D. repens. The index of Minimum Field Infection Rates (MFIR) was calculated.It was founded that the MFIR ranged from 7 prob/1554 total mosquitoes (4,5) to 30 samples (19,3) of PCRpositive mosquitoes for D. immitis and D. repens, respectively. Dual infection with a D. Immitis, and D. repens was detected by PCR in 4 samples (2.6). The proximity of the area to Moscow increased the probability of detection of D. repens and in remote areas, on the contrary, was dominated by D. immitis. The highest rates of total infected mosquitoes were observed in early June and late August.Concluded that the period of the most massive female mosquitoes infected from sick carnivores is at the beginning of August, the greatest risk of infection for healthy people and animals is in the late August — early September. |
| first_indexed | 2024-04-10T01:18:49Z |
| format | Article |
| id | doaj.art-b52ee7bbacf14ddbb9fbd3e59a26b8f3 |
| institution | Directory Open Access Journal |
| issn | 2218-7332 2658-3348 |
| language | Russian |
| last_indexed | 2024-04-10T01:18:49Z |
| publishDate | 2022-12-01 |
| publisher | Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) |
| record_format | Article |
| series | Сеченовский вестник |
| spelling | doaj.art-b52ee7bbacf14ddbb9fbd3e59a26b8f32023-03-13T09:51:37ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482022-12-01045255516MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONSV. M. Rakova0First MSMU named after I.M. SechenovThe aim of the study was to determine the degree of contamination of three kinds of female mosquitoes (Culex, Aedes, Anopheles) in some areas of Moscow and Nizhny Novgorod regions at different times of the warm season of the year (from May to September). Examined adult female mosquitoes collected in the Moscow and Nizhny Novgorod region in the summer of 2010 as potential vectors of Dirofilaria immitis and Dirofilaria repens. The presence of Dirofilaria spp. was detected by polymerase chain reaction using species-specific primers for D. immitis and D. repens. The index of Minimum Field Infection Rates (MFIR) was calculated.It was founded that the MFIR ranged from 7 prob/1554 total mosquitoes (4,5) to 30 samples (19,3) of PCRpositive mosquitoes for D. immitis and D. repens, respectively. Dual infection with a D. Immitis, and D. repens was detected by PCR in 4 samples (2.6). The proximity of the area to Moscow increased the probability of detection of D. repens and in remote areas, on the contrary, was dominated by D. immitis. The highest rates of total infected mosquitoes were observed in early June and late August.Concluded that the period of the most massive female mosquitoes infected from sick carnivores is at the beginning of August, the greatest risk of infection for healthy people and animals is in the late August — early September.https://www.sechenovmedj.com/jour/article/view/822pcrd. repensd. immitismosquitoesanophelesculexaedes |
| spellingShingle | V. M. Rakova MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS Сеченовский вестник pcr d. repens d. immitis mosquitoes anopheles culex aedes |
| title | MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS |
| title_full | MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS |
| title_fullStr | MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS |
| title_full_unstemmed | MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS |
| title_short | MOLECULAR STUDIES OF DIROFILARIA IMMITIS AND DIROFILARIA REPENS PROVIDED WITH THE DIRECT PCR METHOD OF MOSQUITOES RESEARCH IN MOSCOW AND NIZHNY NOVGOROD REGIONS |
| title_sort | molecular studies of dirofilaria immitis and dirofilaria repens provided with the direct pcr method of mosquitoes research in moscow and nizhny novgorod regions |
| topic | pcr d. repens d. immitis mosquitoes anopheles culex aedes |
| url | https://www.sechenovmedj.com/jour/article/view/822 |
| work_keys_str_mv | AT vmrakova molecularstudiesofdirofilariaimmitisanddirofilariarepensprovidedwiththedirectpcrmethodofmosquitoesresearchinmoscowandnizhnynovgorodregions |