Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran
Background: Ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in Iran caused by Babesia ovis, B. motasi, and B. crassa. The aim of this study was to characterize the species of ovine Babesia species isolated from different geographical region of Iran. Metho...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2010-12-01
|
| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/153 |
| _version_ | 1819241346376925184 |
|---|---|
| author | Z Sadeghi Dehkordi S Zakeri S Nabian A Bahonar F Ghasemi F Noorollahi S Rahbari |
| author_facet | Z Sadeghi Dehkordi S Zakeri S Nabian A Bahonar F Ghasemi F Noorollahi S Rahbari |
| author_sort | Z Sadeghi Dehkordi |
| collection | DOAJ |
| description | Background: Ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in Iran caused by Babesia ovis, B. motasi, and B. crassa. The aim of this study was to characterize the species of ovine Babesia species isolated from different geographical region of Iran.
Methods: One hundred fifty four blood samples collected from animals, which demonstrated the pale mucous membranes or hyperthermia. The specimens were transferred to the laboratory and the blood smears stained with Geimsa, the morphological and biometrical data of parasite in any infected erythrocyte have been considered. Extracted DNA from each blood samples were used in PCR and semi nested- PCR in order to confirm the presence of the species.
Results: The results of the PCR assays showed nine (5.85%), 81 (53%) and 18 (11.7%) were distinguished as Babesia, Theileria and mixed infection, respectively. Semi nested- PCR did not confirm the presence of B. motasi.
Conclusion: The causative organism of many cases of haemoprotozoal diseases, which recorded in previous studies, could be B. ovis or Theileria lestoquardi. The result confirmed that B. ovis was only species which causes babesiosis in the study areas. It seems that the biometrical polymorphisms could exist in B. ovis in Iran. This polymorphism could be a main problem in differentiation between B. ovis and B. motasi and it could be dissolved by specific PCR analysis. |
| first_indexed | 2024-12-23T14:22:27Z |
| format | Article |
| id | doaj.art-b5302eee693845e891e40c38ee65059a |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-23T14:22:27Z |
| publishDate | 2010-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-b5302eee693845e891e40c38ee65059a2022-12-21T17:43:46ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2010-12-0154Molecular and Biomorphometrical Identification of Ovine Babesiosis in IranZ Sadeghi Dehkordi0S Zakeri1S Nabian2A Bahonar3F Ghasemi4F Noorollahi5S Rahbari6Dept. of Parasitology, Veterinary Faculty, University of Tehran, IranMalaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranDept. of Parasitology, Veterinary Faculty, University of Tehran, IranDept of Food Hygiene & Quality Control, Veterinary Faculty, University of Tehran, IranMalaria and Vector Research Group (MVRG), Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranParasitic Diseases Control Group, Iranian Veterinary Organization, Tehran, IranDept. of Parasitology, Veterinary Faculty, University of Tehran, IranBackground: Ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in Iran caused by Babesia ovis, B. motasi, and B. crassa. The aim of this study was to characterize the species of ovine Babesia species isolated from different geographical region of Iran. Methods: One hundred fifty four blood samples collected from animals, which demonstrated the pale mucous membranes or hyperthermia. The specimens were transferred to the laboratory and the blood smears stained with Geimsa, the morphological and biometrical data of parasite in any infected erythrocyte have been considered. Extracted DNA from each blood samples were used in PCR and semi nested- PCR in order to confirm the presence of the species. Results: The results of the PCR assays showed nine (5.85%), 81 (53%) and 18 (11.7%) were distinguished as Babesia, Theileria and mixed infection, respectively. Semi nested- PCR did not confirm the presence of B. motasi. Conclusion: The causative organism of many cases of haemoprotozoal diseases, which recorded in previous studies, could be B. ovis or Theileria lestoquardi. The result confirmed that B. ovis was only species which causes babesiosis in the study areas. It seems that the biometrical polymorphisms could exist in B. ovis in Iran. This polymorphism could be a main problem in differentiation between B. ovis and B. motasi and it could be dissolved by specific PCR analysis.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/153Babesia ovisBabesia mutasiPCRSemi nested – PCR |
| spellingShingle | Z Sadeghi Dehkordi S Zakeri S Nabian A Bahonar F Ghasemi F Noorollahi S Rahbari Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran Iranian Journal of Parasitology Babesia ovis Babesia mutasi PCR Semi nested – PCR |
| title | Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran |
| title_full | Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran |
| title_fullStr | Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran |
| title_full_unstemmed | Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran |
| title_short | Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran |
| title_sort | molecular and biomorphometrical identification of ovine babesiosis in iran |
| topic | Babesia ovis Babesia mutasi PCR Semi nested – PCR |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/153 |
| work_keys_str_mv | AT zsadeghidehkordi molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT szakeri molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT snabian molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT abahonar molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT fghasemi molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT fnoorollahi molecularandbiomorphometricalidentificationofovinebabesiosisiniran AT srahbari molecularandbiomorphometricalidentificationofovinebabesiosisiniran |