Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip
We report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-02-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/3/4890 |
| _version_ | 1818015530595909632 |
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| author | Zhiyong Li Yingxin Xu Wei Fang Limin Tong Lei Zhang |
| author_facet | Zhiyong Li Yingxin Xu Wei Fang Limin Tong Lei Zhang |
| author_sort | Zhiyong Li |
| collection | DOAJ |
| description | We report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a detection length of 2.5 cm. Investigated by measuring the fluorescence intensity of rhodamine 6G (R6G), the sensor shows a detection limit down to 100 pM, with excellent reversibility in a concentration range of 0–10 nM. The sensor has also been applied to quantum dot (QD)-labeled streptavidin measurements, yielding a detection sensitivity down to 10 pM for QDs. In addition, the small sample volume (ca. 500 nL), high sampling throughput, and seamless connection between the biconical taper and standard optical fibers offer a number of attractive advantages for chemical and biosensing applications. |
| first_indexed | 2024-04-14T06:58:28Z |
| format | Article |
| id | doaj.art-b53082484bbe422382f06eee2f1cba33 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T06:58:28Z |
| publishDate | 2015-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b53082484bbe422382f06eee2f1cba332022-12-22T02:06:49ZengMDPI AGSensors1424-82202015-02-011534890489810.3390/s150304890s150304890Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic ChipZhiyong Li0Yingxin Xu1Wei Fang2Limin Tong3Lei Zhang4State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, ChinaWe report an ultra-sensitive and robust fluorescence sensor made by using a biconical taper with a waist diameter of 720 nm for both excitation and fluorescence collection. To enhance the stability of the fluorescence sensor, the biconical taper has been embedded in a 125 µm wide microchannel with a detection length of 2.5 cm. Investigated by measuring the fluorescence intensity of rhodamine 6G (R6G), the sensor shows a detection limit down to 100 pM, with excellent reversibility in a concentration range of 0–10 nM. The sensor has also been applied to quantum dot (QD)-labeled streptavidin measurements, yielding a detection sensitivity down to 10 pM for QDs. In addition, the small sample volume (ca. 500 nL), high sampling throughput, and seamless connection between the biconical taper and standard optical fibers offer a number of attractive advantages for chemical and biosensing applications.http://www.mdpi.com/1424-8220/15/3/4890micro/nanofiberbiconical tapermicrofluidic chipfluorescence sensor |
| spellingShingle | Zhiyong Li Yingxin Xu Wei Fang Limin Tong Lei Zhang Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip Sensors micro/nanofiber biconical taper microfluidic chip fluorescence sensor |
| title | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
| title_full | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
| title_fullStr | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
| title_full_unstemmed | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
| title_short | Ultra-Sensitive Nanofiber Fluorescence Detection in a Microfluidic Chip |
| title_sort | ultra sensitive nanofiber fluorescence detection in a microfluidic chip |
| topic | micro/nanofiber biconical taper microfluidic chip fluorescence sensor |
| url | http://www.mdpi.com/1424-8220/15/3/4890 |
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