Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture
In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inse...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/8/1216 |
| _version_ | 1797409354263035904 |
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| author | Magdalena Bauer Magdalena Metzger Marvin Corea Barbara Schädl Johannes Grillari Peter Dungel |
| author_facet | Magdalena Bauer Magdalena Metzger Marvin Corea Barbara Schädl Johannes Grillari Peter Dungel |
| author_sort | Magdalena Bauer |
| collection | DOAJ |
| description | In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-aided design model was realized by extrusion-based 3D printing of polylactic acid filaments (PLA). Improvements in the design of the inserts for easier and more efficient handling were confirmed in cell culture experiments. Cytotoxic effects of the final product were excluded by testing the inserts in accordance with ISO-norm procedures. The final versions of the inserts were tested to generate skin-like 3D scaffolds cultured at an air–liquid interface. Stratification of the epidermal component was demonstrated by histological analyses. In conclusion, here we demonstrate a fast and cost-effective method for 3D-printed inserts suitable for the generation of 3D cell cultures. The system can be set-up with common 3D printers and allows high flexibility for generating customer-tailored cell culture plastics. |
| first_indexed | 2024-03-09T04:13:21Z |
| format | Article |
| id | doaj.art-b530dc8d7b8143a98099902fb25fc01f |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T04:13:21Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-b530dc8d7b8143a98099902fb25fc01f2023-12-03T13:58:49ZengMDPI AGLife2075-17292022-08-01128121610.3390/life12081216Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell CultureMagdalena Bauer0Magdalena Metzger1Marvin Corea2Barbara Schädl3Johannes Grillari4Peter Dungel5Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, 1200 Vienna, AustriaIn skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-aided design model was realized by extrusion-based 3D printing of polylactic acid filaments (PLA). Improvements in the design of the inserts for easier and more efficient handling were confirmed in cell culture experiments. Cytotoxic effects of the final product were excluded by testing the inserts in accordance with ISO-norm procedures. The final versions of the inserts were tested to generate skin-like 3D scaffolds cultured at an air–liquid interface. Stratification of the epidermal component was demonstrated by histological analyses. In conclusion, here we demonstrate a fast and cost-effective method for 3D-printed inserts suitable for the generation of 3D cell cultures. The system can be set-up with common 3D printers and allows high flexibility for generating customer-tailored cell culture plastics.https://www.mdpi.com/2075-1729/12/8/1216tissue engineering3D printingPLAfull-thickness skin modelinsert |
| spellingShingle | Magdalena Bauer Magdalena Metzger Marvin Corea Barbara Schädl Johannes Grillari Peter Dungel Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture Life tissue engineering 3D printing PLA full-thickness skin model insert |
| title | Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture |
| title_full | Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture |
| title_fullStr | Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture |
| title_full_unstemmed | Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture |
| title_short | Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture |
| title_sort | novel 3d printed cell culture inserts for air liquid interface cell culture |
| topic | tissue engineering 3D printing PLA full-thickness skin model insert |
| url | https://www.mdpi.com/2075-1729/12/8/1216 |
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