Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor
Rhamnolipids (RLs), the glycolipidic biosurfactants found initially as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa, are characterized as virulence factors contributing to its pathogenesis infections. However, RLs are also produced by various bacterial species. They consist of a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-08-01
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| Series: | Antibodies |
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| Online Access: | http://www.mdpi.com/2073-4468/2/3/501 |
| _version_ | 1818062013031514112 |
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| author | Anastasia A. Pantazaki Theodora Choli-Papadopoulou Dimitrios C. Giagkas |
| author_facet | Anastasia A. Pantazaki Theodora Choli-Papadopoulou Dimitrios C. Giagkas |
| author_sort | Anastasia A. Pantazaki |
| collection | DOAJ |
| description | Rhamnolipids (RLs), the glycolipidic biosurfactants found initially as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa, are characterized as virulence factors contributing to its pathogenesis infections. However, RLs are also produced by various bacterial species. They consist of a gluconic part, usually containing one or two rhamnoses, and a lipid part, containing one or two hydroxy-fatty acids. In this study, we present both the isolation of RLs from bacterial cultures of the non-pathogenic bacterium Thermus thermophilus as well as the development of the rabbit antibody directed against them. The antibody was titrated and evaluated, in respect of its recognition selectivity. Between both RLs constituents, it specifically recognized only the hydroxydecanoic acid between the fatty acids tested, contrary to rhamnose. The potential of the antibody to recognize both purified RLs and RLs present in crude extracellular media produced by T. thermophilus and Escherichia coli cultures, is evidenced by Dot Blot immuno-reaction. The development of this antibody is addressed in detail, as the sensitive analytical technique, and its potential use would facilitate the implementation of rhamnolipids’ detection, or may be a useful and promising tool for determining these microbial secondary metabolites and virulence factors secreted in extracellular culture media or in biological fluids during infections. |
| first_indexed | 2024-12-10T13:57:27Z |
| format | Article |
| id | doaj.art-b53328d5985847fd98f803035572d55d |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-12-10T13:57:27Z |
| publishDate | 2013-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-b53328d5985847fd98f803035572d55d2022-12-22T01:45:54ZengMDPI AGAntibodies2073-44682013-08-012350151610.3390/antib2030501Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence FactorAnastasia A. PantazakiTheodora Choli-PapadopoulouDimitrios C. GiagkasRhamnolipids (RLs), the glycolipidic biosurfactants found initially as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa, are characterized as virulence factors contributing to its pathogenesis infections. However, RLs are also produced by various bacterial species. They consist of a gluconic part, usually containing one or two rhamnoses, and a lipid part, containing one or two hydroxy-fatty acids. In this study, we present both the isolation of RLs from bacterial cultures of the non-pathogenic bacterium Thermus thermophilus as well as the development of the rabbit antibody directed against them. The antibody was titrated and evaluated, in respect of its recognition selectivity. Between both RLs constituents, it specifically recognized only the hydroxydecanoic acid between the fatty acids tested, contrary to rhamnose. The potential of the antibody to recognize both purified RLs and RLs present in crude extracellular media produced by T. thermophilus and Escherichia coli cultures, is evidenced by Dot Blot immuno-reaction. The development of this antibody is addressed in detail, as the sensitive analytical technique, and its potential use would facilitate the implementation of rhamnolipids’ detection, or may be a useful and promising tool for determining these microbial secondary metabolites and virulence factors secreted in extracellular culture media or in biological fluids during infections.http://www.mdpi.com/2073-4468/2/3/501virulencerhamnolipidsantibodyThermus thermophilus |
| spellingShingle | Anastasia A. Pantazaki Theodora Choli-Papadopoulou Dimitrios C. Giagkas Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor Antibodies virulence rhamnolipids antibody Thermus thermophilus |
| title | Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor |
| title_full | Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor |
| title_fullStr | Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor |
| title_full_unstemmed | Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor |
| title_short | Development of an Antibody for Detection of Rhamnolipids Characterized as a Major Bacterial Virulence Factor |
| title_sort | development of an antibody for detection of rhamnolipids characterized as a major bacterial virulence factor |
| topic | virulence rhamnolipids antibody Thermus thermophilus |
| url | http://www.mdpi.com/2073-4468/2/3/501 |
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