Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine
Species of the genus <i>Paracoccidioides</i> cause a systemic infection in human patients. Yeast cells of <i>Paracoccidioides</i> spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen’s survival in the host. To be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/4/328 |
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| author | Rodrigo Almeida-Paes Marcos Abreu Almeida Lilian Cristiane Baeza Leticia Andrade Mendes Marmello Monique Ramos de Oliveira Trugilho Joshua Daniel Nosanchuk Celia Maria de Almeida Soares Richard Hemmi Valente Rosely Maria Zancopé-Oliveira |
| author_facet | Rodrigo Almeida-Paes Marcos Abreu Almeida Lilian Cristiane Baeza Leticia Andrade Mendes Marmello Monique Ramos de Oliveira Trugilho Joshua Daniel Nosanchuk Celia Maria de Almeida Soares Richard Hemmi Valente Rosely Maria Zancopé-Oliveira |
| author_sort | Rodrigo Almeida-Paes |
| collection | DOAJ |
| description | Species of the genus <i>Paracoccidioides</i> cause a systemic infection in human patients. Yeast cells of <i>Paracoccidioides</i> spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen’s survival in the host. To better understand the metabolic changes that occur in melanized <i>Paracoccidioides</i> spp. cells, a proteomic approach was performed to compare melanized and non-melanized <i>Paracoccidioides brasiliensis</i> and <i>Paracoccidioides lutzii</i> yeast cells. Melanization was induced using L-dihydroxyphenylalanine as a precursor, and quantitative proteomics were performed using reversed-phase nano-chromatography coupled to high-resolution mass spectrometry. When comparing melanized versus non-melanized cells, 1006 and 582 differentially abundant/detected proteins were identified for <i>P. brasiliensis</i> and <i>P. lutzii</i>, respectively. Functional enrichment and comparative analysis revealed 30 important KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways in melanized <i>P. brasiliensis</i> and 18 in <i>P. lutzii</i>, while differentially abundant proteins from non-melanized cells from these species were involved in 21 and 25 enriched pathways, respectively. Melanized cells presented an abundance of additional virulence-associated proteins, such as phospholipase, proteases, superoxide dis-mutases, heat-shock proteins, adhesins, and proteins related to vesicular transport. The results suggest that L-dihydroxyphenylalanine increases the virulence of <i>Paracoccidioides</i> spp. through complex mechanisms involving not only melanin but other virulence factors as well. |
| first_indexed | 2024-03-10T14:24:36Z |
| format | Article |
| id | doaj.art-b5343a75f02a4b9aad7d1973c06097ed |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T14:24:36Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-b5343a75f02a4b9aad7d1973c06097ed2023-11-20T23:07:49ZengMDPI AGJournal of Fungi2309-608X2020-12-016432810.3390/jof6040328Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-DihydroxyphenylalanineRodrigo Almeida-Paes0Marcos Abreu Almeida1Lilian Cristiane Baeza2Leticia Andrade Mendes Marmello3Monique Ramos de Oliveira Trugilho4Joshua Daniel Nosanchuk5Celia Maria de Almeida Soares6Richard Hemmi Valente7Rosely Maria Zancopé-Oliveira8Mycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, BrazilMycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, BrazilCenter for Medical and Pharmaceutical Sciences, State University of Western Paraná, Cascavel, Paraná 85819-110, BrazilMycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilDepartment of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USAMolecular Biology Laboratory, Biological Science Institute, Federal University of Goiás, Samambaia Campus II, ICB2, room 206, Goiânia, Goiás 74690-900, BrazilLaboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, BrazilMycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, BrazilSpecies of the genus <i>Paracoccidioides</i> cause a systemic infection in human patients. Yeast cells of <i>Paracoccidioides</i> spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen’s survival in the host. To better understand the metabolic changes that occur in melanized <i>Paracoccidioides</i> spp. cells, a proteomic approach was performed to compare melanized and non-melanized <i>Paracoccidioides brasiliensis</i> and <i>Paracoccidioides lutzii</i> yeast cells. Melanization was induced using L-dihydroxyphenylalanine as a precursor, and quantitative proteomics were performed using reversed-phase nano-chromatography coupled to high-resolution mass spectrometry. When comparing melanized versus non-melanized cells, 1006 and 582 differentially abundant/detected proteins were identified for <i>P. brasiliensis</i> and <i>P. lutzii</i>, respectively. Functional enrichment and comparative analysis revealed 30 important KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways in melanized <i>P. brasiliensis</i> and 18 in <i>P. lutzii</i>, while differentially abundant proteins from non-melanized cells from these species were involved in 21 and 25 enriched pathways, respectively. Melanized cells presented an abundance of additional virulence-associated proteins, such as phospholipase, proteases, superoxide dis-mutases, heat-shock proteins, adhesins, and proteins related to vesicular transport. The results suggest that L-dihydroxyphenylalanine increases the virulence of <i>Paracoccidioides</i> spp. through complex mechanisms involving not only melanin but other virulence factors as well.https://www.mdpi.com/2309-608X/6/4/328<i>Paracoccidioides</i>melaninL-dihydroxyphenylalanineproteomicsmetabolismvirulence |
| spellingShingle | Rodrigo Almeida-Paes Marcos Abreu Almeida Lilian Cristiane Baeza Leticia Andrade Mendes Marmello Monique Ramos de Oliveira Trugilho Joshua Daniel Nosanchuk Celia Maria de Almeida Soares Richard Hemmi Valente Rosely Maria Zancopé-Oliveira Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine Journal of Fungi <i>Paracoccidioides</i> melanin L-dihydroxyphenylalanine proteomics metabolism virulence |
| title | Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_full | Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_fullStr | Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_full_unstemmed | Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_short | Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine |
| title_sort | beyond melanin proteomics reveals virulence related proteins in i paracoccidioides i i brasiliensis i and i paracoccidioides i i lutzii i yeast cells grown in the presence of l dihydroxyphenylalanine |
| topic | <i>Paracoccidioides</i> melanin L-dihydroxyphenylalanine proteomics metabolism virulence |
| url | https://www.mdpi.com/2309-608X/6/4/328 |
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