The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells
Summary: Recently naive human pluripotent stem cells (hPSCs) have been described that relate to an earlier stage of development than conventional hPSCs. Naive hPSCs remain challenging to generate and authenticate, however. Here we report that Sushi Containing Domain 2 (SUSD2) is a robust cell-surfac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-06-01
|
| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671119300992 |
| _version_ | 1811277765946638336 |
|---|---|
| author | Nicholas Bredenkamp Giuliano Giuseppe Stirparo Jennifer Nichols Austin Smith Ge Guo |
| author_facet | Nicholas Bredenkamp Giuliano Giuseppe Stirparo Jennifer Nichols Austin Smith Ge Guo |
| author_sort | Nicholas Bredenkamp |
| collection | DOAJ |
| description | Summary: Recently naive human pluripotent stem cells (hPSCs) have been described that relate to an earlier stage of development than conventional hPSCs. Naive hPSCs remain challenging to generate and authenticate, however. Here we report that Sushi Containing Domain 2 (SUSD2) is a robust cell-surface marker of naive hPSCs in the embryo and in vitro. SUSD2 transcripts are enriched in the pre-implantation epiblast of human blastocysts and immunostaining shows localization of SUSD2 to KLF17-positive epiblast cells. SUSD2 mRNA is strongly expressed in naive hPSCs but is negligible in other hPSCs. SUSD2 immunostaining of live or fixed cells provides unambiguous discrimination of naive versus conventional hPSCs. SUSD2 staining or flow cytometry enable monitoring of naive hPSCs in maintenance culture, and their isolation and quantification during resetting of conventional hPSCs or somatic cell reprogramming. Thus SUSD2 is a powerful non-invasive tool for reliable identification and purification of the naive hPSC phenotype. : In this article, Guo and colleagues identified SUSD2 as a cell-surface marker highly enriched in naive epiblast in the human blastocyst. SUSD2 staining unambiguously discriminates naive from other hPSCs and provides a powerful tool for identification and isolation of human naive stem cells during resetting of conventional hPSCs or somatic cell reprogramming. Keywords: human naive pluripotent stem cell, cell-surface marker, SUSD2, chemical resetting, somatic cell reprogramming |
| first_indexed | 2024-04-13T00:22:19Z |
| format | Article |
| id | doaj.art-b5368755c01d4c8893f6cb3bf9453675 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-13T00:22:19Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-b5368755c01d4c8893f6cb3bf94536752022-12-22T03:10:44ZengElsevierStem Cell Reports2213-67112019-06-0112612121222The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem CellsNicholas Bredenkamp0Giuliano Giuseppe Stirparo1Jennifer Nichols2Austin Smith3Ge Guo4Wellcome–MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UKWellcome–MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UKWellcome–MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UKWellcome–MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK; Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK; Corresponding authorWellcome–MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK; Corresponding authorSummary: Recently naive human pluripotent stem cells (hPSCs) have been described that relate to an earlier stage of development than conventional hPSCs. Naive hPSCs remain challenging to generate and authenticate, however. Here we report that Sushi Containing Domain 2 (SUSD2) is a robust cell-surface marker of naive hPSCs in the embryo and in vitro. SUSD2 transcripts are enriched in the pre-implantation epiblast of human blastocysts and immunostaining shows localization of SUSD2 to KLF17-positive epiblast cells. SUSD2 mRNA is strongly expressed in naive hPSCs but is negligible in other hPSCs. SUSD2 immunostaining of live or fixed cells provides unambiguous discrimination of naive versus conventional hPSCs. SUSD2 staining or flow cytometry enable monitoring of naive hPSCs in maintenance culture, and their isolation and quantification during resetting of conventional hPSCs or somatic cell reprogramming. Thus SUSD2 is a powerful non-invasive tool for reliable identification and purification of the naive hPSC phenotype. : In this article, Guo and colleagues identified SUSD2 as a cell-surface marker highly enriched in naive epiblast in the human blastocyst. SUSD2 staining unambiguously discriminates naive from other hPSCs and provides a powerful tool for identification and isolation of human naive stem cells during resetting of conventional hPSCs or somatic cell reprogramming. Keywords: human naive pluripotent stem cell, cell-surface marker, SUSD2, chemical resetting, somatic cell reprogramminghttp://www.sciencedirect.com/science/article/pii/S2213671119300992 |
| spellingShingle | Nicholas Bredenkamp Giuliano Giuseppe Stirparo Jennifer Nichols Austin Smith Ge Guo The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells Stem Cell Reports |
| title | The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells |
| title_full | The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells |
| title_fullStr | The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells |
| title_full_unstemmed | The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells |
| title_short | The Cell-Surface Marker Sushi Containing Domain 2 Facilitates Establishment of Human Naive Pluripotent Stem Cells |
| title_sort | cell surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2213671119300992 |
| work_keys_str_mv | AT nicholasbredenkamp thecellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT giulianogiuseppestirparo thecellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT jennifernichols thecellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT austinsmith thecellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT geguo thecellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT nicholasbredenkamp cellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT giulianogiuseppestirparo cellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT jennifernichols cellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT austinsmith cellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells AT geguo cellsurfacemarkersushicontainingdomain2facilitatesestablishmentofhumannaivepluripotentstemcells |