Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress
Currently, salinity is the second biggest challenge in the world after drought and affects all stages of plant growth. The use of environmentally friendly methods such as microorganisms and their derivatives can reduce the destructive effects of salinity stress. A growth chamber experiment was condu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2022.857643/full |
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| author | Iraj Yaghoubian Levini A. Msimbira Donald Lawrence Smith |
| author_facet | Iraj Yaghoubian Levini A. Msimbira Donald Lawrence Smith |
| author_sort | Iraj Yaghoubian |
| collection | DOAJ |
| description | Currently, salinity is the second biggest challenge in the world after drought and affects all stages of plant growth. The use of environmentally friendly methods such as microorganisms and their derivatives can reduce the destructive effects of salinity stress. A growth chamber experiment was conducted to determine the effects of cell-free supernatant (CFS) from Bacillus strains on germination of corn under salinity stress. Corn seeds were subjected to three salinity levels (0, 100 and 150 mM of NaCl), cell-free supernatant of Bacillus strains (U35, U47, U48, U49, and U50) at two levels of dilution (1:50 and 1:250). Germination percentage and rate decreased with increasing salinity toward 150 mM NaCl all together leading to suppressed growth variables for corn seed seedlings including fresh and dry weight of radicle (47.71 and 52.63%, respectively), and shoot (49.52 and 49.25%, respectively), radicle and shoot lengths (39.90 and 66.07%, respectively). Seed vigor index also decreased by 63.04% at 150 mM NaCl. Contrary to salinity, the CFSs of Bacillus strains increased all the growth traits of corn seeds and reduced the negative effects of salinity, especially severe salinity. Ratios of 1:50 and 1: 250 gave best performance for CFSs from U35 and U50, respectively. In general, the highest seed vigor index was obtained by application of 1: 250 CFS from U50. Most germination traits and seed vigor index correlated significantly positive; however, mean germination time was negatively and significantly correlated with the seed vigor index of corn. The results showed that cell-free supernatant use, may as well-helped in changing the ratios of phytohormones, ROS, the activity of antioxidant enzymes and osmotic proteins, hence reduce the negative effects of salinity and improve seed vigor index which eventually increases the ability of plant seedling establishment under saline conditions. |
| first_indexed | 2024-12-13T08:56:33Z |
| format | Article |
| id | doaj.art-b53b21b5c94749a9aebe06afa081c226 |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-12-13T08:56:33Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-b53b21b5c94749a9aebe06afa081c2262022-12-21T23:53:16ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2022-03-01610.3389/fsufs.2022.857643857643Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity StressIraj YaghoubianLevini A. MsimbiraDonald Lawrence SmithCurrently, salinity is the second biggest challenge in the world after drought and affects all stages of plant growth. The use of environmentally friendly methods such as microorganisms and their derivatives can reduce the destructive effects of salinity stress. A growth chamber experiment was conducted to determine the effects of cell-free supernatant (CFS) from Bacillus strains on germination of corn under salinity stress. Corn seeds were subjected to three salinity levels (0, 100 and 150 mM of NaCl), cell-free supernatant of Bacillus strains (U35, U47, U48, U49, and U50) at two levels of dilution (1:50 and 1:250). Germination percentage and rate decreased with increasing salinity toward 150 mM NaCl all together leading to suppressed growth variables for corn seed seedlings including fresh and dry weight of radicle (47.71 and 52.63%, respectively), and shoot (49.52 and 49.25%, respectively), radicle and shoot lengths (39.90 and 66.07%, respectively). Seed vigor index also decreased by 63.04% at 150 mM NaCl. Contrary to salinity, the CFSs of Bacillus strains increased all the growth traits of corn seeds and reduced the negative effects of salinity, especially severe salinity. Ratios of 1:50 and 1: 250 gave best performance for CFSs from U35 and U50, respectively. In general, the highest seed vigor index was obtained by application of 1: 250 CFS from U50. Most germination traits and seed vigor index correlated significantly positive; however, mean germination time was negatively and significantly correlated with the seed vigor index of corn. The results showed that cell-free supernatant use, may as well-helped in changing the ratios of phytohormones, ROS, the activity of antioxidant enzymes and osmotic proteins, hence reduce the negative effects of salinity and improve seed vigor index which eventually increases the ability of plant seedling establishment under saline conditions.https://www.frontiersin.org/articles/10.3389/fsufs.2022.857643/fullBacillus strainscell-free supernatantcornsalinityseed germinationseed vigor index |
| spellingShingle | Iraj Yaghoubian Levini A. Msimbira Donald Lawrence Smith Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress Frontiers in Sustainable Food Systems Bacillus strains cell-free supernatant corn salinity seed germination seed vigor index |
| title | Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress |
| title_full | Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress |
| title_fullStr | Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress |
| title_full_unstemmed | Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress |
| title_short | Cell-Free Supernatant of Bacillus Strains can Improve Seed Vigor Index of Corn (Zea mays L.) Under Salinity Stress |
| title_sort | cell free supernatant of bacillus strains can improve seed vigor index of corn zea mays l under salinity stress |
| topic | Bacillus strains cell-free supernatant corn salinity seed germination seed vigor index |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2022.857643/full |
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