Development of an amperometric-based glucose biosensor to measure the glucose content of fruit.
An amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influence...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0111859 |
| _version_ | 1819108748717719552 |
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| author | Lee Fung Ang Lip Yee Por Mun Fei Yam |
| author_facet | Lee Fung Ang Lip Yee Por Mun Fei Yam |
| author_sort | Lee Fung Ang |
| collection | DOAJ |
| description | An amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influenced by its loading, being suppressed when more than 0.6 mg enzyme was used in the immobilization. The biosensor showing the highest response to glucose utilized 0.21 ml/cm2 thick chitosan membrane. The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential. The introduced biosensor reached a steady-state current at 60 s. The apparent Michaelis-Menten constant ([Formula: see text]) of the biosensor was 14.2350 mM, and its detection limit was 0.05 mM at s/n > 3, determined experimentally. The RSD of repeatability and reproducibility of the biosensor were 2.30% and 3.70%, respectively. The biosensor was showed good stability; it retained ~36% of initial activity after two months of investigation. The performance of the biosensors was evaluated by determining the glucose content in fruit homogenates. Their accuracy was compared to that of a commercial glucose assay kit. There was no significance different between two methods, indicating the introduced biosensor is reliable. |
| first_indexed | 2024-12-22T03:14:52Z |
| format | Article |
| id | doaj.art-b53e4b9882764e6d83611fef64b9896a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:14:52Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b53e4b9882764e6d83611fef64b9896a2022-12-21T18:40:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011185910.1371/journal.pone.0111859Development of an amperometric-based glucose biosensor to measure the glucose content of fruit.Lee Fung AngLip Yee PorMun Fei YamAn amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influenced by its loading, being suppressed when more than 0.6 mg enzyme was used in the immobilization. The biosensor showing the highest response to glucose utilized 0.21 ml/cm2 thick chitosan membrane. The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential. The introduced biosensor reached a steady-state current at 60 s. The apparent Michaelis-Menten constant ([Formula: see text]) of the biosensor was 14.2350 mM, and its detection limit was 0.05 mM at s/n > 3, determined experimentally. The RSD of repeatability and reproducibility of the biosensor were 2.30% and 3.70%, respectively. The biosensor was showed good stability; it retained ~36% of initial activity after two months of investigation. The performance of the biosensors was evaluated by determining the glucose content in fruit homogenates. Their accuracy was compared to that of a commercial glucose assay kit. There was no significance different between two methods, indicating the introduced biosensor is reliable.https://doi.org/10.1371/journal.pone.0111859 |
| spellingShingle | Lee Fung Ang Lip Yee Por Mun Fei Yam Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. PLoS ONE |
| title | Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. |
| title_full | Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. |
| title_fullStr | Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. |
| title_full_unstemmed | Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. |
| title_short | Development of an amperometric-based glucose biosensor to measure the glucose content of fruit. |
| title_sort | development of an amperometric based glucose biosensor to measure the glucose content of fruit |
| url | https://doi.org/10.1371/journal.pone.0111859 |
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