Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study
Background: In contrast to Caucasian melanoma, which has been extensively studied, there are few studies on melanoma in Asian populations. Sporadic studies reported that only 40% of Asian melanoma patients could be druggable, which was much lower than that in Caucasians. More studies are required to...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/1/283 |
| _version_ | 1797626134642294784 |
|---|---|
| author | Yue Li Baoming Wang Chunyang Wang Dandan Zhao Zhengchuang Liu Yanling Niu Xiaojuan Wang Wei Li Jianhua Zhu Houquan Tao Tonghui Ma Tao Li |
| author_facet | Yue Li Baoming Wang Chunyang Wang Dandan Zhao Zhengchuang Liu Yanling Niu Xiaojuan Wang Wei Li Jianhua Zhu Houquan Tao Tonghui Ma Tao Li |
| author_sort | Yue Li |
| collection | DOAJ |
| description | Background: In contrast to Caucasian melanoma, which has been extensively studied, there are few studies on melanoma in Asian populations. Sporadic studies reported that only 40% of Asian melanoma patients could be druggable, which was much lower than that in Caucasians. More studies are required to refine this conclusion. Methods: Chinese melanoma patients (<i>n</i> = 469) were sequentially sequenced by DNA-NGS and RNA-NGS. The genomic alterations were determined, and potentially actionable targets were investigated. Results: Patients with potential druggable targets were identified in 75% of Chinese melanoma patients by DNA-NGS based on OncoKB, which was much higher than in a previous Asian study. <i>NRG1</i> fusions were first identified in melanoma. In addition, up to 11.7% (7/60) of patients in the undruggable group could be recognized as actionable by including RNA-NGS analysis. By comparing the fusion detection rate between DNA-NGS and RNA-NGS, all available samples after DNA-NGS detection were further verified by RNA-NGS. The use of RNA-NGS enhanced the proportion of druggable fusions from 2.56% to 17.27%. In total, the use of RNA-NGS increased the druggable proportion from 75% to 78%. Conclusions: In this study, we systemically analyzed the actionable landscape of melanoma in the largest Asian cohort. In addition, we first demonstrated how DNA and RNA sequential sequencing is essential in bringing clinical benefits to more patients with melanoma. |
| first_indexed | 2024-03-11T10:06:10Z |
| format | Article |
| id | doaj.art-b5414887542f41fea66696808338a65e |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T10:06:10Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-b5414887542f41fea66696808338a65e2023-11-16T15:04:08ZengMDPI AGCancers2072-66942022-12-0115128310.3390/cancers15010283Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter StudyYue Li0Baoming Wang1Chunyang Wang2Dandan Zhao3Zhengchuang Liu4Yanling Niu5Xiaojuan Wang6Wei Li7Jianhua Zhu8Houquan Tao9Tonghui Ma10Tao Li11Harbin Medical University Cancer Hospital, Harbin 150040, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaKey Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou 310014, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaKey Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou 310014, ChinaJichenjunchuang Clinical Laboratory, Hangzhou 310022, ChinaInstitute of Basic Medicine and Cancer (IBMC), Department of Bone and Soft-tissue Surgery, The Cancer Hospital of the University of Chinese Academy of Sciences, Chinese Academy of Sciences, Hangzhou 310022, ChinaBackground: In contrast to Caucasian melanoma, which has been extensively studied, there are few studies on melanoma in Asian populations. Sporadic studies reported that only 40% of Asian melanoma patients could be druggable, which was much lower than that in Caucasians. More studies are required to refine this conclusion. Methods: Chinese melanoma patients (<i>n</i> = 469) were sequentially sequenced by DNA-NGS and RNA-NGS. The genomic alterations were determined, and potentially actionable targets were investigated. Results: Patients with potential druggable targets were identified in 75% of Chinese melanoma patients by DNA-NGS based on OncoKB, which was much higher than in a previous Asian study. <i>NRG1</i> fusions were first identified in melanoma. In addition, up to 11.7% (7/60) of patients in the undruggable group could be recognized as actionable by including RNA-NGS analysis. By comparing the fusion detection rate between DNA-NGS and RNA-NGS, all available samples after DNA-NGS detection were further verified by RNA-NGS. The use of RNA-NGS enhanced the proportion of druggable fusions from 2.56% to 17.27%. In total, the use of RNA-NGS increased the druggable proportion from 75% to 78%. Conclusions: In this study, we systemically analyzed the actionable landscape of melanoma in the largest Asian cohort. In addition, we first demonstrated how DNA and RNA sequential sequencing is essential in bringing clinical benefits to more patients with melanoma.https://www.mdpi.com/2072-6694/15/1/283melanomaDNA-NGSRNA-NGSsequencetarget |
| spellingShingle | Yue Li Baoming Wang Chunyang Wang Dandan Zhao Zhengchuang Liu Yanling Niu Xiaojuan Wang Wei Li Jianhua Zhu Houquan Tao Tonghui Ma Tao Li Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study Cancers melanoma DNA-NGS RNA-NGS sequence target |
| title | Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study |
| title_full | Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study |
| title_fullStr | Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study |
| title_full_unstemmed | Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study |
| title_short | Genomic and Transcriptional Profiling of Chinese Melanoma Patients Enhanced Potentially Druggable Targets: A Multicenter Study |
| title_sort | genomic and transcriptional profiling of chinese melanoma patients enhanced potentially druggable targets a multicenter study |
| topic | melanoma DNA-NGS RNA-NGS sequence target |
| url | https://www.mdpi.com/2072-6694/15/1/283 |
| work_keys_str_mv | AT yueli genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT baomingwang genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT chunyangwang genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT dandanzhao genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT zhengchuangliu genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT yanlingniu genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT xiaojuanwang genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT weili genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT jianhuazhu genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT houquantao genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT tonghuima genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy AT taoli genomicandtranscriptionalprofilingofchinesemelanomapatientsenhancedpotentiallydruggabletargetsamulticenterstudy |