S1R agonist modulates rat platelet eicosanoid synthesis and aggregation
Sigma-1 receptor (S1R) is detected in different cell types and can regulate intracellular signaling pathways. S1R plays a role in the pathomechanism of diseases and the regulation of neurotransmitters. Fluvoxamine can bind to S1R and reduce the serotonin uptake of neurons and platelets. We therefore...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-07-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2021.1981843 |
| _version_ | 1797684161458208768 |
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| author | Sándor Váczi L. Barna A. Harazin M. Mészáros G. Porkoláb Á. Zvara R. Ónody I. Földesi S. Veszelka B. Penke L. Fülöp M. A. Deli Z. Mezei |
| author_facet | Sándor Váczi L. Barna A. Harazin M. Mészáros G. Porkoláb Á. Zvara R. Ónody I. Földesi S. Veszelka B. Penke L. Fülöp M. A. Deli Z. Mezei |
| author_sort | Sándor Váczi |
| collection | DOAJ |
| description | Sigma-1 receptor (S1R) is detected in different cell types and can regulate intracellular signaling pathways. S1R plays a role in the pathomechanism of diseases and the regulation of neurotransmitters. Fluvoxamine can bind to S1R and reduce the serotonin uptake of neurons and platelets. We therefore hypothesized that platelets express S1R, which can modify platelet function. The expression of the SIGMAR1 gene in rat platelets was examined with a reverse transcription polymerase chain reaction and a quantitative polymerase chain reaction. The receptor was also visualized by immunostaining and confocal laser scanning microscopy. The effect of S1R agonist PRE-084 on the eicosanoid synthesis of isolated rat platelets and ADP- and AA-induced platelet aggregation was examined. S1R was detected in rat platelets both at gene and protein levels. Pretreatment with PRE-084 of resting platelets induced elevation of eicosanoid synthesis. The rate of elevation in thromboxane B2 and prostaglandin D2 synthesis was similar, but the production of prostaglandin E2 was higher. The concentration-response curve showed a sigmoidal form. The most effective concentration of the agonist was 2 µM. PRE-084 increased the quantity of cyclooxygenase-1 as detected by ELISA. PRE-084 also elevated the ADP- and AA-induced platelet aggregation. S1R of platelets might regulate physiological or pathological functions. |
| first_indexed | 2024-03-12T00:25:28Z |
| format | Article |
| id | doaj.art-b544eccdc73147498e95cb95716ca9f2 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:25:28Z |
| publishDate | 2022-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-b544eccdc73147498e95cb95716ca9f22023-09-15T10:38:10ZengTaylor & Francis GroupPlatelets0953-71041369-16352022-07-0133570971810.1080/09537104.2021.19818431981843S1R agonist modulates rat platelet eicosanoid synthesis and aggregationSándor Váczi0L. Barna1A. Harazin2M. Mészáros3G. Porkoláb4Á. Zvara5R. Ónody6I. Földesi7S. Veszelka8B. Penke9L. Fülöp10M. A. Deli11Z. Mezei12University of SzegedInstitute of Biophysics, Biological Research CentreInstitute of Biophysics, Biological Research CentreInstitute of Biophysics, Biological Research CentreInstitute of Biophysics, Biological Research CentreInstitute of Genetics, Biological Research CentreUniversity of SzegedUniversity of SzegedInstitute of Biophysics, Biological Research CentreUniversity of SzegedUniversity of SzegedInstitute of Biophysics, Biological Research CentreUniversity of SzegedSigma-1 receptor (S1R) is detected in different cell types and can regulate intracellular signaling pathways. S1R plays a role in the pathomechanism of diseases and the regulation of neurotransmitters. Fluvoxamine can bind to S1R and reduce the serotonin uptake of neurons and platelets. We therefore hypothesized that platelets express S1R, which can modify platelet function. The expression of the SIGMAR1 gene in rat platelets was examined with a reverse transcription polymerase chain reaction and a quantitative polymerase chain reaction. The receptor was also visualized by immunostaining and confocal laser scanning microscopy. The effect of S1R agonist PRE-084 on the eicosanoid synthesis of isolated rat platelets and ADP- and AA-induced platelet aggregation was examined. S1R was detected in rat platelets both at gene and protein levels. Pretreatment with PRE-084 of resting platelets induced elevation of eicosanoid synthesis. The rate of elevation in thromboxane B2 and prostaglandin D2 synthesis was similar, but the production of prostaglandin E2 was higher. The concentration-response curve showed a sigmoidal form. The most effective concentration of the agonist was 2 µM. PRE-084 increased the quantity of cyclooxygenase-1 as detected by ELISA. PRE-084 also elevated the ADP- and AA-induced platelet aggregation. S1R of platelets might regulate physiological or pathological functions.http://dx.doi.org/10.1080/09537104.2021.1981843aggregationeicosanoidplateletspre-084sigma-1 receptor |
| spellingShingle | Sándor Váczi L. Barna A. Harazin M. Mészáros G. Porkoláb Á. Zvara R. Ónody I. Földesi S. Veszelka B. Penke L. Fülöp M. A. Deli Z. Mezei S1R agonist modulates rat platelet eicosanoid synthesis and aggregation Platelets aggregation eicosanoid platelets pre-084 sigma-1 receptor |
| title | S1R agonist modulates rat platelet eicosanoid synthesis and aggregation |
| title_full | S1R agonist modulates rat platelet eicosanoid synthesis and aggregation |
| title_fullStr | S1R agonist modulates rat platelet eicosanoid synthesis and aggregation |
| title_full_unstemmed | S1R agonist modulates rat platelet eicosanoid synthesis and aggregation |
| title_short | S1R agonist modulates rat platelet eicosanoid synthesis and aggregation |
| title_sort | s1r agonist modulates rat platelet eicosanoid synthesis and aggregation |
| topic | aggregation eicosanoid platelets pre-084 sigma-1 receptor |
| url | http://dx.doi.org/10.1080/09537104.2021.1981843 |
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