Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model
Background Systemic acquired resistance (SAR) protects plants against a wide variety of pathogens. In recent decades, numerous studies have focused on the induction of SAR, but its molecular mechanisms remain largely unknown. Methods We used a metabolomics approach based on ultra-high-performance li...
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PeerJ Inc.
2022-04-01
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| Online Access: | https://peerj.com/articles/13293.pdf |
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| author | Jian-Bo Song Rui-Ke Huang Miao-Jie Guo Qian Zhou Rui Guo Shu-Yuan Zhang Jing-Wen Yao Ya-Ni Bai Xuan Huang |
| author_facet | Jian-Bo Song Rui-Ke Huang Miao-Jie Guo Qian Zhou Rui Guo Shu-Yuan Zhang Jing-Wen Yao Ya-Ni Bai Xuan Huang |
| author_sort | Jian-Bo Song |
| collection | DOAJ |
| description | Background Systemic acquired resistance (SAR) protects plants against a wide variety of pathogens. In recent decades, numerous studies have focused on the induction of SAR, but its molecular mechanisms remain largely unknown. Methods We used a metabolomics approach based on ultra-high-performance liquid chromatographic (UPLC) and mass spectrometric (MS) techniques to identify SAR-related lipid metabolites in an Arabidopsis thaliana model. Multiple statistical analyses were used to identify the differentially regulated metabolites. Results Numerous lipids were implicated as potential factors in both plant basal resistance and SAR; these include species of phosphatidic acid (PA), monogalactosyldiacylglycerol (MGDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG). Conclusions Our findings indicate that lipids accumulated in both local and systemic leaves, while other lipids only accumulated in local leaves or in systemic leaves. PA (16:0_18:2), PE (34:5) and PE (16:0_18:2) had higher levels in both local leaves inoculated with Psm ES4326 or Psm avrRpm1 and systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. PC (32:5) had high levels in leaves inoculated with Psm ES4326. Other differentially regulated metabolites, including PA (18:2_18:2), PA (16:0_18:3), PA (18:3_18:2), PE (16:0_18:3), PE (16:1_16:1), PE (34:4) and TGs showed higher levels in systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. These findings will help direct future studies on the molecular mechanisms of SAR. |
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| language | English |
| last_indexed | 2024-03-09T06:57:28Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-b547d7e2625049b8bc9fbc2bce1526ac2023-12-03T10:02:50ZengPeerJ Inc.PeerJ2167-83592022-04-0110e1329310.7717/peerj.13293Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana modelJian-Bo Song0Rui-Ke Huang1Miao-Jie Guo2Qian Zhou3Rui Guo4Shu-Yuan Zhang5Jing-Wen Yao6Ya-Ni Bai7Xuan Huang8College of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaShanghai Omicsspace Biotechnology Co.Ltd., Shanghai, Shanghai, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaCollege of Life Sciences, Northwest University, Shaanxi, Xi’an, ChinaBackground Systemic acquired resistance (SAR) protects plants against a wide variety of pathogens. In recent decades, numerous studies have focused on the induction of SAR, but its molecular mechanisms remain largely unknown. Methods We used a metabolomics approach based on ultra-high-performance liquid chromatographic (UPLC) and mass spectrometric (MS) techniques to identify SAR-related lipid metabolites in an Arabidopsis thaliana model. Multiple statistical analyses were used to identify the differentially regulated metabolites. Results Numerous lipids were implicated as potential factors in both plant basal resistance and SAR; these include species of phosphatidic acid (PA), monogalactosyldiacylglycerol (MGDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG). Conclusions Our findings indicate that lipids accumulated in both local and systemic leaves, while other lipids only accumulated in local leaves or in systemic leaves. PA (16:0_18:2), PE (34:5) and PE (16:0_18:2) had higher levels in both local leaves inoculated with Psm ES4326 or Psm avrRpm1 and systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. PC (32:5) had high levels in leaves inoculated with Psm ES4326. Other differentially regulated metabolites, including PA (18:2_18:2), PA (16:0_18:3), PA (18:3_18:2), PE (16:0_18:3), PE (16:1_16:1), PE (34:4) and TGs showed higher levels in systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. These findings will help direct future studies on the molecular mechanisms of SAR.https://peerj.com/articles/13293.pdfSystemic acquired resistanceLipidsMonogalactosyldiacylglycerolPhosphatidic acidPhosphatidylethanolamineTriacylglycerol |
| spellingShingle | Jian-Bo Song Rui-Ke Huang Miao-Jie Guo Qian Zhou Rui Guo Shu-Yuan Zhang Jing-Wen Yao Ya-Ni Bai Xuan Huang Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model PeerJ Systemic acquired resistance Lipids Monogalactosyldiacylglycerol Phosphatidic acid Phosphatidylethanolamine Triacylglycerol |
| title | Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model |
| title_full | Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model |
| title_fullStr | Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model |
| title_full_unstemmed | Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model |
| title_short | Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model |
| title_sort | lipids associated with plant bacteria interaction identified using a metabolomics approach in an arabidopsis thaliana model |
| topic | Systemic acquired resistance Lipids Monogalactosyldiacylglycerol Phosphatidic acid Phosphatidylethanolamine Triacylglycerol |
| url | https://peerj.com/articles/13293.pdf |
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