Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12
Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.02010/full |
| _version_ | 1818379549476388864 |
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| author | Kihyun Kim Yoonji Lee Areum Ha Ji-In Kim Ae Ran Park Nan Hee Yu Hokyoung Son Gyung Ja Choi Hae Woong Park Chul Won Lee Theresa Lee Yin-Won Lee Jin-Cheol Kim |
| author_facet | Kihyun Kim Yoonji Lee Areum Ha Ji-In Kim Ae Ran Park Nan Hee Yu Hokyoung Son Gyung Ja Choi Hae Woong Park Chul Won Lee Theresa Lee Yin-Won Lee Jin-Cheol Kim |
| author_sort | Kihyun Kim |
| collection | DOAJ |
| description | Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB. B. amyloliquefaciens JCK-12 produces several cyclic lipopeptides (CLPs) including iturin A, fengycin, and surfactin. Iturin A inhibits spore germination of F. graminearum. Fengycin or surfactin alone did not display any inhibitory activity against spore germination at concentrations less than 30 μg/ml, but a mixture of iturin A, fengycin, and surfactin showed a remarkable synergistic inhibitory effect on F. graminearum spore germination. The fermentation broth and formulation of B. amyloliquefaciens JCK-12 strain reduced the disease incidence of FHB in wheat. Furthermore, co-application of B. amyloliquefaciens JCK-12 and chemical fungicides resulted in synergistic in vitro antifungal effects and significant disease control efficacy against FHB under greenhouse and field conditions, suggesting that B. amyloliquefaciens JCK-12 has a strong chemosensitizing effect. The synergistic antifungal effect of B. amyloliquefaciens JCK-12 and chemical fungicides in combination may result from the cell wall damage and altered cell membrane permeability in the phytopathogenic fungi caused by the CLP mixtures and subsequent increased sensitivity of F. graminearum to fungicides. In addition, B. amyloliquefaciens JCK-12 showed the potential to reduce trichothecenes mycotoxin production. The results of this study indicate that B. amyloliquefaciens JCK-12 could be used as an available biocontrol agent or as a chemosensitizer to chemical fungicides for controlling FHB disease and as a strategy for preventing the contamination of harvested crops with mycotoxins. |
| first_indexed | 2024-12-14T02:04:33Z |
| format | Article |
| id | doaj.art-b54887fe9cac4fcb965175ba2acbb2af |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T02:04:33Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b54887fe9cac4fcb965175ba2acbb2af2022-12-21T23:20:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-11-01810.3389/fpls.2017.02010312275Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12Kihyun Kim0Yoonji Lee1Areum Ha2Ji-In Kim3Ae Ran Park4Nan Hee Yu5Hokyoung Son6Gyung Ja Choi7Hae Woong Park8Chul Won Lee9Theresa Lee10Yin-Won Lee11Jin-Cheol Kim12Department of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaDepartment of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaDepartment of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaDepartment of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaDepartment of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon, South KoreaWorld Institute of Kimchi, Korea Food Research Institute, Gwangju, South KoreaDepartment of Chemistry, College of Natural Sciences, Chonnam National University, Gwangju, South KoreaMicrobial Safety Team, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Wanju, South KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaDepartment of Agricultural Chemistry, Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, South KoreaFusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB. B. amyloliquefaciens JCK-12 produces several cyclic lipopeptides (CLPs) including iturin A, fengycin, and surfactin. Iturin A inhibits spore germination of F. graminearum. Fengycin or surfactin alone did not display any inhibitory activity against spore germination at concentrations less than 30 μg/ml, but a mixture of iturin A, fengycin, and surfactin showed a remarkable synergistic inhibitory effect on F. graminearum spore germination. The fermentation broth and formulation of B. amyloliquefaciens JCK-12 strain reduced the disease incidence of FHB in wheat. Furthermore, co-application of B. amyloliquefaciens JCK-12 and chemical fungicides resulted in synergistic in vitro antifungal effects and significant disease control efficacy against FHB under greenhouse and field conditions, suggesting that B. amyloliquefaciens JCK-12 has a strong chemosensitizing effect. The synergistic antifungal effect of B. amyloliquefaciens JCK-12 and chemical fungicides in combination may result from the cell wall damage and altered cell membrane permeability in the phytopathogenic fungi caused by the CLP mixtures and subsequent increased sensitivity of F. graminearum to fungicides. In addition, B. amyloliquefaciens JCK-12 showed the potential to reduce trichothecenes mycotoxin production. The results of this study indicate that B. amyloliquefaciens JCK-12 could be used as an available biocontrol agent or as a chemosensitizer to chemical fungicides for controlling FHB disease and as a strategy for preventing the contamination of harvested crops with mycotoxins.http://journal.frontiersin.org/article/10.3389/fpls.2017.02010/fullBacillus amyloliquefaciens JCK-12Fusarium head blightcyclic lipopeptidessynergistic antifungal effectchemosensitization |
| spellingShingle | Kihyun Kim Yoonji Lee Areum Ha Ji-In Kim Ae Ran Park Nan Hee Yu Hokyoung Son Gyung Ja Choi Hae Woong Park Chul Won Lee Theresa Lee Yin-Won Lee Jin-Cheol Kim Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 Frontiers in Plant Science Bacillus amyloliquefaciens JCK-12 Fusarium head blight cyclic lipopeptides synergistic antifungal effect chemosensitization |
| title | Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 |
| title_full | Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 |
| title_fullStr | Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 |
| title_full_unstemmed | Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 |
| title_short | Chemosensitization of Fusarium graminearum to Chemical Fungicides Using Cyclic Lipopeptides Produced by Bacillus amyloliquefaciens Strain JCK-12 |
| title_sort | chemosensitization of fusarium graminearum to chemical fungicides using cyclic lipopeptides produced by bacillus amyloliquefaciens strain jck 12 |
| topic | Bacillus amyloliquefaciens JCK-12 Fusarium head blight cyclic lipopeptides synergistic antifungal effect chemosensitization |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.02010/full |
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