Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae.
Monochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4729689?pdf=render |
| _version_ | 1811205661004922880 |
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| author | Songqing Wu Xiaoli Zhu Zhaoxia Liu Ensi Shao Carballar-Lejarazú Rebeca Yajie Guo Yueting Xiong Yani Mou Runxue Xu Xia Hu Guanghong Liang Shuangquan Zou Xiong Guan Feiping Zhang |
| author_facet | Songqing Wu Xiaoli Zhu Zhaoxia Liu Ensi Shao Carballar-Lejarazú Rebeca Yajie Guo Yueting Xiong Yani Mou Runxue Xu Xia Hu Guanghong Liang Shuangquan Zou Xiong Guan Feiping Zhang |
| author_sort | Songqing Wu |
| collection | DOAJ |
| description | Monochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management of the disease. However, there is no deep sequence analysis of Monochamus alternatus Hope that describes the transcriptome and no information is available about gene function of this insect vector. We used next generation sequencing technology to sequence the whole fourth instar larva transcriptome of Monochamus alternatus Hope and successfully built a Monochamus alternatus Hope transcriptome database. In total, 105,612 unigenes were assigned for Gene Ontology (GO) terms, information for 16,730 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs) database, and 13,024 unigenes matched with 224 predicted pathways in the Kyoto Encyclopedia of Genes and Genome (KEGG). In addition, genes related to putative insecticide resistance-related genes, RNAi, the Bt receptor, intestinal digestive enzymes, possible future insect control targets and immune-related molecules are described. This study provides valuable basic information that can be used as a gateway to develop new molecular tools for Monochamus alternatus Hope control strategies. |
| first_indexed | 2024-04-12T03:35:51Z |
| format | Article |
| id | doaj.art-b54f87dc00dd4a699d2790820b25b80b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:35:51Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b54f87dc00dd4a699d2790820b25b80b2022-12-22T03:49:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014785510.1371/journal.pone.0147855Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae.Songqing WuXiaoli ZhuZhaoxia LiuEnsi ShaoCarballar-Lejarazú RebecaYajie GuoYueting XiongYani MouRunxue XuXia HuGuanghong LiangShuangquan ZouXiong GuanFeiping ZhangMonochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management of the disease. However, there is no deep sequence analysis of Monochamus alternatus Hope that describes the transcriptome and no information is available about gene function of this insect vector. We used next generation sequencing technology to sequence the whole fourth instar larva transcriptome of Monochamus alternatus Hope and successfully built a Monochamus alternatus Hope transcriptome database. In total, 105,612 unigenes were assigned for Gene Ontology (GO) terms, information for 16,730 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs) database, and 13,024 unigenes matched with 224 predicted pathways in the Kyoto Encyclopedia of Genes and Genome (KEGG). In addition, genes related to putative insecticide resistance-related genes, RNAi, the Bt receptor, intestinal digestive enzymes, possible future insect control targets and immune-related molecules are described. This study provides valuable basic information that can be used as a gateway to develop new molecular tools for Monochamus alternatus Hope control strategies.http://europepmc.org/articles/PMC4729689?pdf=render |
| spellingShingle | Songqing Wu Xiaoli Zhu Zhaoxia Liu Ensi Shao Carballar-Lejarazú Rebeca Yajie Guo Yueting Xiong Yani Mou Runxue Xu Xia Hu Guanghong Liang Shuangquan Zou Xiong Guan Feiping Zhang Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. PLoS ONE |
| title | Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. |
| title_full | Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. |
| title_fullStr | Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. |
| title_full_unstemmed | Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. |
| title_short | Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae. |
| title_sort | identification of genes relevant to pesticides and biology from global transcriptome data of monochamus alternatus hope coleoptera cerambycidae larvae |
| url | http://europepmc.org/articles/PMC4729689?pdf=render |
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