A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation
The spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non...
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MDPI AG
2021-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/11/1680 |
| _version_ | 1797510244533796864 |
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| author | Ricardo Pereira José M. Leitão |
| author_facet | Ricardo Pereira José M. Leitão |
| author_sort | Ricardo Pereira |
| collection | DOAJ |
| description | The spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non-rogue (wild type) plants, produce exclusively rogue plants in the first and all subsequent generations. The fact that the wild phenotype disappears forever, is in clear contradiction with the Mendelian rules of inheritance, a situation that impedes the positional cloning of genes involved in this epigenetic phenomenon. One way of overcoming this obstacle is the identification of plant genotypes harboring naturally occurring or artificially induced neutral alleles, non-sensitive to paramutation. So far, such alleles have never been described for the pea rogue paramutation. Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. Progreta, and the completely unusual fixation of the induced non-rogue phenotype through several generations. The reversion of the methylation status of two previously identified differentially methylated genomic sequences in the induced non-rogue mutant, confirms that the rogue paramutation is accompanied by alterations in DNA methylation. Nevertheless, unexpectedly, the induced non-rogue mutant showed to be still sensitive to paramutation. |
| first_indexed | 2024-03-10T05:28:50Z |
| format | Article |
| id | doaj.art-b5509d96a1f648488178dc2c5cf01673 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T05:28:50Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-b5509d96a1f648488178dc2c5cf016732023-11-22T23:27:15ZengMDPI AGGenes2073-44252021-10-011211168010.3390/genes12111680A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue ParamutationRicardo Pereira0José M. Leitão1Laboratory of Genomics and Genetic Improvement, MED, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, PortugalLaboratory of Genomics and Genetic Improvement, MED, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, PortugalThe spontaneously emerging rogue phenotype in peas (<i>Pisum sativum</i> L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non-rogue (wild type) plants, produce exclusively rogue plants in the first and all subsequent generations. The fact that the wild phenotype disappears forever, is in clear contradiction with the Mendelian rules of inheritance, a situation that impedes the positional cloning of genes involved in this epigenetic phenomenon. One way of overcoming this obstacle is the identification of plant genotypes harboring naturally occurring or artificially induced neutral alleles, non-sensitive to paramutation. So far, such alleles have never been described for the pea rogue paramutation. Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. Progreta, and the completely unusual fixation of the induced non-rogue phenotype through several generations. The reversion of the methylation status of two previously identified differentially methylated genomic sequences in the induced non-rogue mutant, confirms that the rogue paramutation is accompanied by alterations in DNA methylation. Nevertheless, unexpectedly, the induced non-rogue mutant showed to be still sensitive to paramutation.https://www.mdpi.com/2073-4425/12/11/1680peaPisumparamutationrogue phenotype1-ethyl-1-nitrosoureaENU |
| spellingShingle | Ricardo Pereira José M. Leitão A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation Genes pea Pisum paramutation rogue phenotype 1-ethyl-1-nitrosourea ENU |
| title | A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation |
| title_full | A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation |
| title_fullStr | A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation |
| title_full_unstemmed | A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation |
| title_short | A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (<i>Pisum sativum</i> L.) Is Still Sensitive to the Rogue Paramutation |
| title_sort | non rogue mutant line induced by enu mutagenesis in paramutated rogue peas i pisum sativum i l is still sensitive to the rogue paramutation |
| topic | pea Pisum paramutation rogue phenotype 1-ethyl-1-nitrosourea ENU |
| url | https://www.mdpi.com/2073-4425/12/11/1680 |
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