Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization
Abstract Background Scientifically, isoflavones from Pueraria thomsonii Benth possess diverse pharmacological activities and have been used to treat various diseases. In vitro propagation of callus has contributed to the reliability for large-scale production of target compounds. However, the factor...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-03-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-022-00220-4 |
| _version_ | 1818315377930665984 |
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| author | Yu Li Pachaiyappan Saravana Kumar Yu Liu Jiao Qiu Yalan Ran Mingyuan Yuan Xinyue Fang Xuhui Tan Renjun Zhao Ji zhu Meijun He |
| author_facet | Yu Li Pachaiyappan Saravana Kumar Yu Liu Jiao Qiu Yalan Ran Mingyuan Yuan Xinyue Fang Xuhui Tan Renjun Zhao Ji zhu Meijun He |
| author_sort | Yu Li |
| collection | DOAJ |
| description | Abstract Background Scientifically, isoflavones from Pueraria thomsonii Benth possess diverse pharmacological activities and have been used to treat various diseases. In vitro propagation of callus has contributed to the reliability for large-scale production of target compounds. However, the factors affecting the biosynthesis of major isoflavones daidzin, puerarin and daidzein in the callus culture of P. thomsonii are still not known. Therefore, we aimed to enhance the in vitro production of daidzin, puerarin and daidzein by optimizing three independent factors such as temperature, NAA and 6-BA concentrations. Results Our findings showed that the optimal concentrations for in vitro biomass production and efficient synthesis of puerarin, daidzin and daidzein were found to be 0.158%, 0.463% and 0.057%, respectively. In addition, the HPLC fingerprint with chemo-metrics analysis was constructed by linear regression of the puerarin, daidzin and daidzein which was found to be in the range of 1.0–36.0, 5.0–72.0 and 1.0–15.0 mg/mL and the LODs and LOQs were found to be 0.15, 0.52, 0.35 and 0.28, 1.50, 0.50 mg/mL for puerarin, daidzin and daidzein, respectively. Surprisingly, our results were also in agreement with the concentration obtained from the model verification for optimal and efficient production of puerarin, daidzin and daidzein which was found to be 0.162%, 0.458% and 0.049%, respectively. Conclusions In summary, our present investigation provides new insights that could facilitate the enhanced production of valuable isoflavones in P. thomsonii using plant cell cultures treated with appropriate elicitor combinations and temperature. As far as the authors are concerned, this is the first report on production of daidzin, puerarin and daidzein at higher yield at laboratory level for a wide range of applications in future food, medicinal and pharmaceutical companies. |
| first_indexed | 2024-12-13T09:04:35Z |
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| id | doaj.art-b552e818f2a24bff8cb0d60ce8db1e18 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-12-13T09:04:35Z |
| publishDate | 2022-03-01 |
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| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-b552e818f2a24bff8cb0d60ce8db1e182022-12-21T23:53:06ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432022-03-0111111310.1186/s43088-022-00220-4Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimizationYu Li0Pachaiyappan Saravana Kumar1Yu Liu2Jiao Qiu3Yalan Ran4Mingyuan Yuan5Xinyue Fang6Xuhui Tan7Renjun Zhao8Ji zhu9Meijun He10Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesDepartment of Central Hospital of Tujia and Miao Autonomous Prefecture, Hubei University of MedicineAgricultural and Rural Bureau of Enshi Tujia and Miao Autonomous PrefectureInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesInstitute of Chinese Herbal Medicines, Hubei Academy of Agricultural SciencesAbstract Background Scientifically, isoflavones from Pueraria thomsonii Benth possess diverse pharmacological activities and have been used to treat various diseases. In vitro propagation of callus has contributed to the reliability for large-scale production of target compounds. However, the factors affecting the biosynthesis of major isoflavones daidzin, puerarin and daidzein in the callus culture of P. thomsonii are still not known. Therefore, we aimed to enhance the in vitro production of daidzin, puerarin and daidzein by optimizing three independent factors such as temperature, NAA and 6-BA concentrations. Results Our findings showed that the optimal concentrations for in vitro biomass production and efficient synthesis of puerarin, daidzin and daidzein were found to be 0.158%, 0.463% and 0.057%, respectively. In addition, the HPLC fingerprint with chemo-metrics analysis was constructed by linear regression of the puerarin, daidzin and daidzein which was found to be in the range of 1.0–36.0, 5.0–72.0 and 1.0–15.0 mg/mL and the LODs and LOQs were found to be 0.15, 0.52, 0.35 and 0.28, 1.50, 0.50 mg/mL for puerarin, daidzin and daidzein, respectively. Surprisingly, our results were also in agreement with the concentration obtained from the model verification for optimal and efficient production of puerarin, daidzin and daidzein which was found to be 0.162%, 0.458% and 0.049%, respectively. Conclusions In summary, our present investigation provides new insights that could facilitate the enhanced production of valuable isoflavones in P. thomsonii using plant cell cultures treated with appropriate elicitor combinations and temperature. As far as the authors are concerned, this is the first report on production of daidzin, puerarin and daidzein at higher yield at laboratory level for a wide range of applications in future food, medicinal and pharmaceutical companies.https://doi.org/10.1186/s43088-022-00220-4Pueraria thomsoniiCallus inductionIsoflavonesElicitorsHPLC analysisRSM |
| spellingShingle | Yu Li Pachaiyappan Saravana Kumar Yu Liu Jiao Qiu Yalan Ran Mingyuan Yuan Xinyue Fang Xuhui Tan Renjun Zhao Ji zhu Meijun He Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization Beni-Suef University Journal of Basic and Applied Sciences Pueraria thomsonii Callus induction Isoflavones Elicitors HPLC analysis RSM |
| title | Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization |
| title_full | Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization |
| title_fullStr | Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization |
| title_full_unstemmed | Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization |
| title_short | Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization |
| title_sort | tailoring enhanced production and identification of isoflavones in the callus cultures of pueraria thomsonii benth and its model verification using response surface methodology rsm a combined in vitro and statistical optimization |
| topic | Pueraria thomsonii Callus induction Isoflavones Elicitors HPLC analysis RSM |
| url | https://doi.org/10.1186/s43088-022-00220-4 |
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