Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease
ABSTRACT: Although intranasal bovine respiratory disease (BRD) vaccines containing live attenuated virus elicit greater stimulation of local humoral immunity response, they can mimic a viral infection, responsible for reducing innate defense during the establishment of vaccine-induced immunity. Prob...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Patologia Animal (CBPA)
2024-04-01
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| Series: | Pesquisa Veterinária Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100108&lng=en&tlng=en |
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| author | Heloisa G. Bertagnon Caroline R. Depaoli Sarah N. Oliveira Bianca Milla Bruna F. Zdepski Gabriela Garbossa |
| author_facet | Heloisa G. Bertagnon Caroline R. Depaoli Sarah N. Oliveira Bianca Milla Bruna F. Zdepski Gabriela Garbossa |
| author_sort | Heloisa G. Bertagnon |
| collection | DOAJ |
| description | ABSTRACT: Although intranasal bovine respiratory disease (BRD) vaccines containing live attenuated virus elicit greater stimulation of local humoral immunity response, they can mimic a viral infection, responsible for reducing innate defense during the establishment of vaccine-induced immunity. Probiotics containing Saccharomyces cerevisiae or Enterococcus faecium reduced the occurrence of BRD in neonatal calves challenged with the bovine respiratory syncytial virus (BRSV). Furthermore, the probiotics potentiated the humoral immune response after vaccination in murine models, raising the question of whether they could have the same effect in calves. This study aimed to verify if the probiotic containing E. faecium and S. cerevisiae attenuates the inflammation caused by the vaccine against BRD in the respiratory tract region in calves. Twenty-four healthy Jersey calves, aged 6 to 7 months old, were divided into the groups: control (C), supplemented (S), vaccinated (V), and supplemented and vaccinated (SV), with six animals in each of them. Supplemented groups (S and SV) received S. cerevisiae and E. faecium once a day on D-15 for 51 days (Probios precise®, Ouro Fino®, 2g/day/animal). Vaccinated groups (V and SV) received a single dose of an intranasal BRD vaccine on day 0 (Inforce®, Zoetis®, 1ml/ nostril). The control group was not supplemented or vaccined. Irritation of the respiratory tract and bronchoalveolar (BA) evaluations: cytology, phagocyte function, and IgA were measured on D-15, D3, D7, and D21. The vaccinated groups showed greater irritation of the nasopharynx and trachea. However, only Group V showed a reduction in BA phagocyte function and an increase in cellularity by a neutrophil influx in the BA region. Regarding IgA BA, SV showed the greatest increase, followed by S and V, concerning C. We conclude that isolated supplementation with E. faecium and S. cerevisiae promoted increased production of BA IgA. In association with the vaccine, the supplementation attenuated the inflammation of the respiratory tract produced by the vaccine itself, avoiding the reduction of phagocyte function BA, besides potentiating the humoral immune response of the vaccine containing live attenuated virus against BRD. |
| first_indexed | 2024-04-24T08:55:41Z |
| format | Article |
| id | doaj.art-b554951997654f6184f83667aab91d02 |
| institution | Directory Open Access Journal |
| issn | 1678-5150 |
| language | English |
| last_indexed | 2024-04-24T08:55:41Z |
| publishDate | 2024-04-01 |
| publisher | Colégio Brasileiro de Patologia Animal (CBPA) |
| record_format | Article |
| series | Pesquisa Veterinária Brasileira |
| spelling | doaj.art-b554951997654f6184f83667aab91d022024-04-16T07:42:27ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502024-04-014410.1590/1678-5150-pvb-7374Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory diseaseHeloisa G. Bertagnonhttps://orcid.org/0000-0002-6983-1000Caroline R. Depaolihttps://orcid.org/0000-0003-1152-1824Sarah N. Oliveirahttps://orcid.org/0000-0002-0123-2001Bianca Millahttps://orcid.org/0000-0001-7514-1711Bruna F. Zdepskihttps://orcid.org/0000-0002-8704-4385Gabriela Garbossahttps://orcid.org/0000-0001-5127-6670ABSTRACT: Although intranasal bovine respiratory disease (BRD) vaccines containing live attenuated virus elicit greater stimulation of local humoral immunity response, they can mimic a viral infection, responsible for reducing innate defense during the establishment of vaccine-induced immunity. Probiotics containing Saccharomyces cerevisiae or Enterococcus faecium reduced the occurrence of BRD in neonatal calves challenged with the bovine respiratory syncytial virus (BRSV). Furthermore, the probiotics potentiated the humoral immune response after vaccination in murine models, raising the question of whether they could have the same effect in calves. This study aimed to verify if the probiotic containing E. faecium and S. cerevisiae attenuates the inflammation caused by the vaccine against BRD in the respiratory tract region in calves. Twenty-four healthy Jersey calves, aged 6 to 7 months old, were divided into the groups: control (C), supplemented (S), vaccinated (V), and supplemented and vaccinated (SV), with six animals in each of them. Supplemented groups (S and SV) received S. cerevisiae and E. faecium once a day on D-15 for 51 days (Probios precise®, Ouro Fino®, 2g/day/animal). Vaccinated groups (V and SV) received a single dose of an intranasal BRD vaccine on day 0 (Inforce®, Zoetis®, 1ml/ nostril). The control group was not supplemented or vaccined. Irritation of the respiratory tract and bronchoalveolar (BA) evaluations: cytology, phagocyte function, and IgA were measured on D-15, D3, D7, and D21. The vaccinated groups showed greater irritation of the nasopharynx and trachea. However, only Group V showed a reduction in BA phagocyte function and an increase in cellularity by a neutrophil influx in the BA region. Regarding IgA BA, SV showed the greatest increase, followed by S and V, concerning C. We conclude that isolated supplementation with E. faecium and S. cerevisiae promoted increased production of BA IgA. In association with the vaccine, the supplementation attenuated the inflammation of the respiratory tract produced by the vaccine itself, avoiding the reduction of phagocyte function BA, besides potentiating the humoral immune response of the vaccine containing live attenuated virus against BRD.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100108&lng=en&tlng=enAlveolar macrophagereactive oxygen speciesIgAbronchoscopypneumoniacalf |
| spellingShingle | Heloisa G. Bertagnon Caroline R. Depaoli Sarah N. Oliveira Bianca Milla Bruna F. Zdepski Gabriela Garbossa Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease Pesquisa Veterinária Brasileira Alveolar macrophage reactive oxygen species IgA bronchoscopy pneumonia calf |
| title | Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| title_full | Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| title_fullStr | Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| title_full_unstemmed | Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| title_short | Immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| title_sort | immunostimulation of bronchoalveolar response in calves vaccinated against bovine respiratory disease |
| topic | Alveolar macrophage reactive oxygen species IgA bronchoscopy pneumonia calf |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2024000100108&lng=en&tlng=en |
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