Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter
The effects of potential synbiotic chitosan and <i>Acinetobacter</i> KU011TH mixture on growth performance, immune response, and <i>A. hydrophila</i> resistance were investigated for the first time. The control group was fed a basal diet (A), and group B was given the formula...
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MDPI AG
2023-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1807 |
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| author | Pisey Say Sukkrit Nimikul Anurak Bunnoy Uthairat Na-Nakorn Prapansak Srisapoome |
| author_facet | Pisey Say Sukkrit Nimikul Anurak Bunnoy Uthairat Na-Nakorn Prapansak Srisapoome |
| author_sort | Pisey Say |
| collection | DOAJ |
| description | The effects of potential synbiotic chitosan and <i>Acinetobacter</i> KU011TH mixture on growth performance, immune response, and <i>A. hydrophila</i> resistance were investigated for the first time. The control group was fed a basal diet (A), and group B was given the formula B diet that was supplemented with chitosan at 20 mL/kg diet via top dressing. The other synbiotic groups, C, D, and E, were top-dressed with the target probiotics at 1 × 10<sup>8</sup>, 1 × 10<sup>9</sup>, and 1 × 10<sup>10</sup> CFU/kg diet, respectively, and coated with the same concentration of chitosan. Fish were continuously fed the five different feeds for 16 weeks during winter. At the end of the trial, the growth parameters of the test groups did not significantly differ from those of the control (<i>p</i> > 0.05). All the symbiotic-chitosan treatments significantly increased various hematological and serum immune parameters. Moreover, the expression levels of immune-related genes were strongly elevated in the head kidney and spleen, whereas upregulated expression was observed in the liver and whole blood (<i>p</i> < 0.05). Survival analysis indicated that fish in groups B and C showed significantly higher survival (84.33 ± 2.21 and 79.50 ± 6.34%) than those in groups A, D and E (55.33 ± 8.82%–74.00 ± 6.50) (<i>p</i> < 0.05) after injection with <i>A. hydrophila</i> for 14 days. |
| first_indexed | 2024-03-11T00:48:11Z |
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| id | doaj.art-b5637daadee747b9b87a0de76bc2a19d |
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| language | English |
| last_indexed | 2024-03-11T00:48:11Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b5637daadee747b9b87a0de76bc2a19d2023-11-18T20:36:43ZengMDPI AGMicroorganisms2076-26072023-07-01117180710.3390/microorganisms11071807Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during WinterPisey Say0Sukkrit Nimikul1Anurak Bunnoy2Uthairat Na-Nakorn3Prapansak Srisapoome4Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandDepartment of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandLaboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandLaboratory of Aquatic Animal Genetics, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandLaboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, ThailandThe effects of potential synbiotic chitosan and <i>Acinetobacter</i> KU011TH mixture on growth performance, immune response, and <i>A. hydrophila</i> resistance were investigated for the first time. The control group was fed a basal diet (A), and group B was given the formula B diet that was supplemented with chitosan at 20 mL/kg diet via top dressing. The other synbiotic groups, C, D, and E, were top-dressed with the target probiotics at 1 × 10<sup>8</sup>, 1 × 10<sup>9</sup>, and 1 × 10<sup>10</sup> CFU/kg diet, respectively, and coated with the same concentration of chitosan. Fish were continuously fed the five different feeds for 16 weeks during winter. At the end of the trial, the growth parameters of the test groups did not significantly differ from those of the control (<i>p</i> > 0.05). All the symbiotic-chitosan treatments significantly increased various hematological and serum immune parameters. Moreover, the expression levels of immune-related genes were strongly elevated in the head kidney and spleen, whereas upregulated expression was observed in the liver and whole blood (<i>p</i> < 0.05). Survival analysis indicated that fish in groups B and C showed significantly higher survival (84.33 ± 2.21 and 79.50 ± 6.34%) than those in groups A, D and E (55.33 ± 8.82%–74.00 ± 6.50) (<i>p</i> < 0.05) after injection with <i>A. hydrophila</i> for 14 days.https://www.mdpi.com/2076-2607/11/7/1807<i>Acinetobacter</i> KU011TH<i>Aeromonas hydrophila</i>chitosan<i>Clarias</i> catfishdisease resistancehybrid catfish |
| spellingShingle | Pisey Say Sukkrit Nimikul Anurak Bunnoy Uthairat Na-Nakorn Prapansak Srisapoome Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter Microorganisms <i>Acinetobacter</i> KU011TH <i>Aeromonas hydrophila</i> chitosan <i>Clarias</i> catfish disease resistance hybrid catfish |
| title | Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter |
| title_full | Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter |
| title_fullStr | Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter |
| title_full_unstemmed | Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter |
| title_short | Long-Term Application of a Synbiotic Chitosan and <i>Acinetobacter</i> KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (<i>Clarias gariepinus</i> × <i>C. macrocephalus</i>) during Winter |
| title_sort | long term application of a synbiotic chitosan and i acinetobacter i ku011th mixture on the growth performance health status and disease resistance of hybrid catfish i clarias gariepinus i i c macrocephalus i during winter |
| topic | <i>Acinetobacter</i> KU011TH <i>Aeromonas hydrophila</i> chitosan <i>Clarias</i> catfish disease resistance hybrid catfish |
| url | https://www.mdpi.com/2076-2607/11/7/1807 |
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