Ultra-Fast Middle-Up Reversed Phase Liquid Chromatography Analysis of Complex Bispecific Antibodies Obtained in Less Than One Minute

Ultra-Fast Middle-Up Reversed Phase Liquid Chromatography Analysis of Complex Bispecific Antibodies Obtained in Less Than One Minute

This work illustrates the benefits and limitations of using ultra-short reversed phase liquid chromatography (RPLC) columns for the characterization of various complex bispecific antibodies after prolonged thermal stress at the middle-up level of analysis. First, we have demonstrated that alternativ...

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Bibliographic Details
Main Authors: Amarande Murisier, Valentina D’Atri, Sebastian Pirner, Vincent Larraillet, Szabolcs Fekete, Matthew Lauber, Davy Guillarme
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Pharmaceutics
Subjects:
ultra-short columns
bispecific antibodies
reversed-phase liquid chromatography
improved selectivity
method robustness
Online Access:https://www.mdpi.com/1999-4923/14/11/2315

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