Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes
Background and purpose: Cilostazol is a prescription medication for intermittent claudication in patients with peripheral artery disease. Previous research has demonstrated that this 2-oxoquinoline derivative possesses antithrombotic, vasodilator, antimitogenic, and antioxidant effects. Cilostazol e...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2023-12-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-19430-en.pdf |
| _version_ | 1827400067615555584 |
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| author | Mohammad Shokrzadeh Mahboube Rahmati Elahe Gharehkhani Rouzbeh Zare Parsa Pourmohammadi Shaghayegh Aghajanshakeri |
| author_facet | Mohammad Shokrzadeh Mahboube Rahmati Elahe Gharehkhani Rouzbeh Zare Parsa Pourmohammadi Shaghayegh Aghajanshakeri |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background and purpose: Cilostazol is a prescription medication for intermittent claudication in patients with peripheral artery disease. Previous research has demonstrated that this 2-oxoquinoline derivative possesses antithrombotic, vasodilator, antimitogenic, and antioxidant effects. Cilostazol exerts its products through the inhibition of PDE3 activity and the prevention of cAMP degradation. The present study aimed to examine the protective properties of cilostazol against carboplatin-induced cytotoxicity and genotoxicity in Beas-2B and human blood lymphocyte cells.
Materials and methods: cells were pre-treated with different concentrations of cilostazol (5, 25, 50, and 100 μM) with carboplatin at an optimum cytotoxic dosage (9.2 µM). The MTT and micronucleus assays were used to assess cytotoxicity and genotoxicity. Statistical analysis was performed using the one-way ANOVA in Prism Ver. 8 software.
Results: The cytotoxic effect of carboplatin was dose-dependent, as evidenced by the 48h culture treatment with concentrations of 0.3, 1, 3, 10, and 30 µM. Pre-treatment of cilostazol at 25, 50, and 100 µM with carboplatin at 9.2 µM enhanced cell viability in Beas-2B cells compared to the carboplatin alone as positive control. Additionally, cilostazol at 50 and 100 µM showed its potent genoprotective effects via micronucleus assay against carboplatin at IC50 at blood lymphocyte cells.
Conclusion: Cilostazol provided conceivable protective effects by modulating cytotoxicity and genotoxicity induced by carboplatin in Beas-2B and human blood lymphocyte cells. |
| first_indexed | 2024-03-08T19:55:26Z |
| format | Article |
| id | doaj.art-b56d5cad42064d37bfd302ff20abdec9 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-03-08T19:55:26Z |
| publishDate | 2023-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-b56d5cad42064d37bfd302ff20abdec92023-12-24T06:57:31ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792023-12-01332272436Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood LymphocytesMohammad Shokrzadeh0Mahboube Rahmati1Elahe Gharehkhani2Rouzbeh Zare3Parsa Pourmohammadi4Shaghayegh Aghajanshakeri5 Professor, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Pharmacy Student, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Ramsar, Iran PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran and Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Cilostazol is a prescription medication for intermittent claudication in patients with peripheral artery disease. Previous research has demonstrated that this 2-oxoquinoline derivative possesses antithrombotic, vasodilator, antimitogenic, and antioxidant effects. Cilostazol exerts its products through the inhibition of PDE3 activity and the prevention of cAMP degradation. The present study aimed to examine the protective properties of cilostazol against carboplatin-induced cytotoxicity and genotoxicity in Beas-2B and human blood lymphocyte cells. Materials and methods: cells were pre-treated with different concentrations of cilostazol (5, 25, 50, and 100 μM) with carboplatin at an optimum cytotoxic dosage (9.2 µM). The MTT and micronucleus assays were used to assess cytotoxicity and genotoxicity. Statistical analysis was performed using the one-way ANOVA in Prism Ver. 8 software. Results: The cytotoxic effect of carboplatin was dose-dependent, as evidenced by the 48h culture treatment with concentrations of 0.3, 1, 3, 10, and 30 µM. Pre-treatment of cilostazol at 25, 50, and 100 µM with carboplatin at 9.2 µM enhanced cell viability in Beas-2B cells compared to the carboplatin alone as positive control. Additionally, cilostazol at 50 and 100 µM showed its potent genoprotective effects via micronucleus assay against carboplatin at IC50 at blood lymphocyte cells. Conclusion: Cilostazol provided conceivable protective effects by modulating cytotoxicity and genotoxicity induced by carboplatin in Beas-2B and human blood lymphocyte cells.http://jmums.mazums.ac.ir/article-1-19430-en.pdfcilostazolcarboplatinmicronucleus assaycytotoxicity |
| spellingShingle | Mohammad Shokrzadeh Mahboube Rahmati Elahe Gharehkhani Rouzbeh Zare Parsa Pourmohammadi Shaghayegh Aghajanshakeri Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes Journal of Mazandaran University of Medical Sciences cilostazol carboplatin micronucleus assay cytotoxicity |
| title | Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes |
| title_full | Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes |
| title_fullStr | Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes |
| title_full_unstemmed | Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes |
| title_short | Pretreatment with Cilostazol Attenuates Carboplatin Induced Cytotoxicity and Genotoxicity in Bronchial Epithelial Cells (Beas-2B) and Human Blood Lymphocytes |
| title_sort | pretreatment with cilostazol attenuates carboplatin induced cytotoxicity and genotoxicity in bronchial epithelial cells beas 2b and human blood lymphocytes |
| topic | cilostazol carboplatin micronucleus assay cytotoxicity |
| url | http://jmums.mazums.ac.ir/article-1-19430-en.pdf |
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