In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol
Objective: To explore the mechanism by which nonylphenol (NP) interferes with male infertility through evaluation of its effects on epididymal sperm of adult male rats. Methods: Twenty four Sprague–Dawley (SD) rats were used as epididymal sperm donors. Previously rats were administrated with NP (0,...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-09-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015000172 |
| _version_ | 1819205818781794304 |
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| author | Ansoumane Kourouma Peng Duan Hady Keita Aidogie Osamuyimen Suqin Qi Chao Quan Tingting Yu Kedi Yang |
| author_facet | Ansoumane Kourouma Peng Duan Hady Keita Aidogie Osamuyimen Suqin Qi Chao Quan Tingting Yu Kedi Yang |
| author_sort | Ansoumane Kourouma |
| collection | DOAJ |
| description | Objective: To explore the mechanism by which nonylphenol (NP) interferes with male infertility through evaluation of its effects on epididymal sperm of adult male rats.
Methods: Twenty four Sprague–Dawley (SD) rats were used as epididymal sperm donors. Previously rats were administrated with NP (0, 2, 10 and 50 mg/kg) body weight respectively in corn oil every forty-eight hours by intraperitoneal injection for 30 days. Computer assisted sperm analysis (CASA) was used to determine parameters of sperm. The sperm morphology examination was conducted with a high resolution microscope.
Results: Results indicated that exposure to NP has no effect on body weight, while testes weights were significantly decreased. Computer assisted sperm analysis (CASA) showed significant decline in the percentage of motile spermatozoa (P < 0.001), STR and LIN (P < 0.01), significant increase in ALH (P < 0.001), while significant decline in BCF (P < 0.001) respectively. Plasma LDH was significantly increased while; plasma γ-GT activity was significantly decreased. H2O2 production and malondialdehyde (MDA) were significantly increased. The Plasma CAT, GSH-Px and SOD activities were significantly decreased.
Conclusions: This concludes that NP leads oxidative stress in the epididymal sperm of rats. Moreover, NP can disrupt sperm motility and alterations in the sperm morphology. |
| first_indexed | 2024-12-23T04:57:45Z |
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| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-12-23T04:57:45Z |
| publishDate | 2015-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-b56dfde608db4aadaa743ee27f2c338c2022-12-21T17:59:17ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002015-09-014316917810.1016/j.apjr.2015.05.002In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenolAnsoumane Kourouma0Peng Duan1Hady Keita2Aidogie Osamuyimen3Suqin Qi4Chao Quan5Tingting Yu6Kedi Yang7MOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaMOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaUniversidad Tecnológica del Valle de Toluca, Carretera del Departamento del D.F. km 7.5, Santa María Atarasquillo Lerma, MexicoSchool of Medicine and Health Management, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, ChinaMOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaMOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaMOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaMOE Key Lab of Environmental and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, ChinaObjective: To explore the mechanism by which nonylphenol (NP) interferes with male infertility through evaluation of its effects on epididymal sperm of adult male rats. Methods: Twenty four Sprague–Dawley (SD) rats were used as epididymal sperm donors. Previously rats were administrated with NP (0, 2, 10 and 50 mg/kg) body weight respectively in corn oil every forty-eight hours by intraperitoneal injection for 30 days. Computer assisted sperm analysis (CASA) was used to determine parameters of sperm. The sperm morphology examination was conducted with a high resolution microscope. Results: Results indicated that exposure to NP has no effect on body weight, while testes weights were significantly decreased. Computer assisted sperm analysis (CASA) showed significant decline in the percentage of motile spermatozoa (P < 0.001), STR and LIN (P < 0.01), significant increase in ALH (P < 0.001), while significant decline in BCF (P < 0.001) respectively. Plasma LDH was significantly increased while; plasma γ-GT activity was significantly decreased. H2O2 production and malondialdehyde (MDA) were significantly increased. The Plasma CAT, GSH-Px and SOD activities were significantly decreased. Conclusions: This concludes that NP leads oxidative stress in the epididymal sperm of rats. Moreover, NP can disrupt sperm motility and alterations in the sperm morphology.http://www.sciencedirect.com/science/article/pii/S2305050015000172NonylphenolEpididymisSpermsAntioxidantsCASAROS |
| spellingShingle | Ansoumane Kourouma Peng Duan Hady Keita Aidogie Osamuyimen Suqin Qi Chao Quan Tingting Yu Kedi Yang In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol Asian Pacific Journal of Reproduction Nonylphenol Epididymis Sperms Antioxidants CASA ROS |
| title | In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| title_full | In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| title_fullStr | In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| title_full_unstemmed | In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| title_short | In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| title_sort | in vitro assessment of ros on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol |
| topic | Nonylphenol Epididymis Sperms Antioxidants CASA ROS |
| url | http://www.sciencedirect.com/science/article/pii/S2305050015000172 |
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