Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer
Background and objective During the occurring and developing of tumor, tumor-educated platelets mRNA profiles were altered. Since platelets are anuclear, the level of mRNAs is probably post-transcriptional regulated by the splicing maturation of pre-mRNA and alternative splicing. Apoptotic chromatin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2018-09-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2018.09.05 |
| _version_ | 1811221915925217280 |
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| author | Linlin XUE Li XIE Xingguo SONG Xianrang SONG |
| author_facet | Linlin XUE Li XIE Xingguo SONG Xianrang SONG |
| author_sort | Linlin XUE |
| collection | DOAJ |
| description | Background and objective During the occurring and developing of tumor, tumor-educated platelets mRNA profiles were altered. Since platelets are anuclear, the level of mRNAs is probably post-transcriptional regulated by the splicing maturation of pre-mRNA and alternative splicing. Apoptotic chromatin condensation inducer 1 (ACIN1) has been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) and was involved in mRNA metabolism associated with splicing. This study analyzed the expression of ACIN1 mRNA in platelets, and explored its potential as a biomarker of lung cancer. Methods 156 patients with lung cancer and 58 healthy controls in Shandong Cancer Hospital were collected. We isolated platelet pellets by low-speed centrifugation and extracted total RNA. The expression of ACIN1 mRNA in platelets was detected by RT-PCR, the results were analyzed statistically. And the relationship between expression of ACIN1 mRNA and clinical factors were also analyzed. Results The expression level of ACIN1 mRNA in platelets of patients with lung cancer was significantly higher than that in platelets of healthy controls (P=0.015). The ROC curve showed that the area under the curve of ACIN1 mRNA for detecting lung cancer were 0.608. The expression of ACIN1 mRNA in platelets of lung cancer has no significant relationship with age, gender, pathological type and metastasis or not (P>0.05). Conclusion ACIN1 mRNA was highly expressed in platelets of lung cancer patients, and the detection of its expression level might have potential clinical value for the diagnosis of lung cancer. |
| first_indexed | 2024-04-12T08:08:04Z |
| format | Article |
| id | doaj.art-b56f569fc3364faf9cdc5cba9120a66d |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-12T08:08:04Z |
| publishDate | 2018-09-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-b56f569fc3364faf9cdc5cba9120a66d2022-12-22T03:41:05ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872018-09-0121967768110.3779/j.issn.1009-3419.2018.09.05Expression and Significance of ACIN1 mRNA in Platelets of Lung CancerLinlin XUE0Li XIE1Xingguo SONG2Xianrang SONG3Shandong Cancer Hospital Affiliated to Shandong University, Jinan 250117, China; Shandong Academy of Medical Sciences, Jinan 250002, ChinaShandong Cancer Hospital Affiliated to Shandong University, Jinan 250117, China; Shandong Academy of Medical Sciences, Jinan 250002, ChinaShandong Cancer Hospital Affiliated to Shandong University, Jinan 250117, China; Shandong Academy of Medical Sciences, Jinan 250002, ChinaShandong Cancer Hospital Affiliated to Shandong University, Jinan 250117, China; Shandong Academy of Medical Sciences, Jinan 250002, ChinaBackground and objective During the occurring and developing of tumor, tumor-educated platelets mRNA profiles were altered. Since platelets are anuclear, the level of mRNAs is probably post-transcriptional regulated by the splicing maturation of pre-mRNA and alternative splicing. Apoptotic chromatin condensation inducer 1 (ACIN1) has been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) and was involved in mRNA metabolism associated with splicing. This study analyzed the expression of ACIN1 mRNA in platelets, and explored its potential as a biomarker of lung cancer. Methods 156 patients with lung cancer and 58 healthy controls in Shandong Cancer Hospital were collected. We isolated platelet pellets by low-speed centrifugation and extracted total RNA. The expression of ACIN1 mRNA in platelets was detected by RT-PCR, the results were analyzed statistically. And the relationship between expression of ACIN1 mRNA and clinical factors were also analyzed. Results The expression level of ACIN1 mRNA in platelets of patients with lung cancer was significantly higher than that in platelets of healthy controls (P=0.015). The ROC curve showed that the area under the curve of ACIN1 mRNA for detecting lung cancer were 0.608. The expression of ACIN1 mRNA in platelets of lung cancer has no significant relationship with age, gender, pathological type and metastasis or not (P>0.05). Conclusion ACIN1 mRNA was highly expressed in platelets of lung cancer patients, and the detection of its expression level might have potential clinical value for the diagnosis of lung cancer.http://dx.doi.org/10.3779/j.issn.1009-3419.2018.09.05PlateletLung neoplasmsSpliceosomeACIN1mRNA |
| spellingShingle | Linlin XUE Li XIE Xingguo SONG Xianrang SONG Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer Chinese Journal of Lung Cancer Platelet Lung neoplasms Spliceosome ACIN1 mRNA |
| title | Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer |
| title_full | Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer |
| title_fullStr | Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer |
| title_full_unstemmed | Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer |
| title_short | Expression and Significance of ACIN1 mRNA in Platelets of Lung Cancer |
| title_sort | expression and significance of acin1 mrna in platelets of lung cancer |
| topic | Platelet Lung neoplasms Spliceosome ACIN1 mRNA |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2018.09.05 |
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