Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium
IntroductionFlower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-lik...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1133616/full |
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| author | Yaolei Mi Ruikun He Huihua Wan Xiangxiao Meng Di Liu Wenjun Huang Yanjun Zhang Zubaida Yousaf Hongwen Huang Shilin Chen Shilin Chen Ying Wang Wei Sun Wei Sun |
| author_facet | Yaolei Mi Ruikun He Huihua Wan Xiangxiao Meng Di Liu Wenjun Huang Yanjun Zhang Zubaida Yousaf Hongwen Huang Shilin Chen Shilin Chen Ying Wang Wei Sun Wei Sun |
| author_sort | Yaolei Mi |
| collection | DOAJ |
| description | IntroductionFlower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-like petals and petaloid sepals.MethodsIn this work, the anthocyanidins of eight different Epimedium species with different floral pigmentation phenotypes were analyzed using HPLC. Twelve genes involved in anthocyanin biosynthesis were cloned and sequenced, and their expression was quantified.ResultsThe expression levels of the catalytic enzyme genes DFR and ANS were significantly decreased in four species showing loss of floral pigmentation. Complementation of EsF3’H and EsDFR in corresponding Arabidopsis mutants together with overexpression of EsF3’5’H in wild type Arabidopsis analysis revealed that these genes were functional at the protein level, based on the accumulation of anthocyanin pigments.DiscussionThese results strongly suggest that transcriptional regulatory changes determine the loss of anthocyanins to be convergent in the floral tissue of Epimedium species. |
| first_indexed | 2024-04-09T21:03:21Z |
| format | Article |
| id | doaj.art-b57231ae39d74cec881d8299261d8b8b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T21:03:21Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-b57231ae39d74cec881d8299261d8b8b2023-03-29T07:06:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11336161133616Genetic and molecular analysis of the anthocyanin pigmentation pathway in EpimediumYaolei Mi0Ruikun He1Huihua Wan2Xiangxiao Meng3Di Liu4Wenjun Huang5Yanjun Zhang6Zubaida Yousaf7Hongwen Huang8Shilin Chen9Shilin Chen10Ying Wang11Wei Sun12Wei Sun13Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaBy-Health Institute of Nutrition and health. By-health Co., Ltd., Guangzhou, Guangdong, ChinaLaboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaLaboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, ChinaKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, ChinaDepartment of Botany, Lahore College for Women University, Lahore, PakistanLushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, Jiangxi, ChinaLaboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaSouth China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, ChinaLaboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaInstitute of Herbgenomics, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaIntroductionFlower color is an ideal trait for studying the molecular basis for phenotypic variations in natural populations of species. Epimedium (Berberidaceae) species exhibit a wide range of flower colors resulting from the varied accumulation of anthocyanins and other pigments in their spur-like petals and petaloid sepals.MethodsIn this work, the anthocyanidins of eight different Epimedium species with different floral pigmentation phenotypes were analyzed using HPLC. Twelve genes involved in anthocyanin biosynthesis were cloned and sequenced, and their expression was quantified.ResultsThe expression levels of the catalytic enzyme genes DFR and ANS were significantly decreased in four species showing loss of floral pigmentation. Complementation of EsF3’H and EsDFR in corresponding Arabidopsis mutants together with overexpression of EsF3’5’H in wild type Arabidopsis analysis revealed that these genes were functional at the protein level, based on the accumulation of anthocyanin pigments.DiscussionThese results strongly suggest that transcriptional regulatory changes determine the loss of anthocyanins to be convergent in the floral tissue of Epimedium species.https://www.frontiersin.org/articles/10.3389/fpls.2023.1133616/fullgene expressionEpimediumanthocyaninspursepal |
| spellingShingle | Yaolei Mi Ruikun He Huihua Wan Xiangxiao Meng Di Liu Wenjun Huang Yanjun Zhang Zubaida Yousaf Hongwen Huang Shilin Chen Shilin Chen Ying Wang Wei Sun Wei Sun Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium Frontiers in Plant Science gene expression Epimedium anthocyanin spur sepal |
| title | Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium |
| title_full | Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium |
| title_fullStr | Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium |
| title_full_unstemmed | Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium |
| title_short | Genetic and molecular analysis of the anthocyanin pigmentation pathway in Epimedium |
| title_sort | genetic and molecular analysis of the anthocyanin pigmentation pathway in epimedium |
| topic | gene expression Epimedium anthocyanin spur sepal |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1133616/full |
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