The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge
Patients with pathological nipple discharge (PND) often undergo local surgical procedures because standard radiologic imaging fails to identify the underlying cause. MicroRNA (MiRNA) expression analysis of nipple fluid holds potential for distinguishing between breast diseases. This study aimed to c...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1812 |
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| author | Seher Makineli Menno R. Vriens Arjen J. Witkamp Paul J. van Diest Cathy B. Moelans |
| author_facet | Seher Makineli Menno R. Vriens Arjen J. Witkamp Paul J. van Diest Cathy B. Moelans |
| author_sort | Seher Makineli |
| collection | DOAJ |
| description | Patients with pathological nipple discharge (PND) often undergo local surgical procedures because standard radiologic imaging fails to identify the underlying cause. MicroRNA (MiRNA) expression analysis of nipple fluid holds potential for distinguishing between breast diseases. This study aimed to compare miRNA expression levels between nipple fluids from patients with PND to identify possible relevant miRNAs that could differentiate between intraductal papillomas and no abnormalities in the breast tissue. Nipple fluid samples from patients with PND without radiological and pathological suspicion for malignancy who underwent a ductoscopy procedure were analyzed. We used univariate and multivariate regression analyses to identify nipple fluid miRNAs differing between pathologically confirmed papillomas and breast tissue without abnormalities. A total of 27 nipple fluid samples from patients with PND were included for miRNA expression analysis. Out of the 22 miRNAs examined, only miR-145-5p was significantly differentially expressed (upregulated) in nipple fluid from patients with an intraductal papilloma compared to patients showing no breast abnormalities (OR 4.76, <i>p</i> = 0.046), with a diagnostic accuracy of 92%. miR-145-5p expression in nipple fluid differs for intraductal papillomas and breast tissue without abnormalities and, therefore, has potential as a diagnostic marker to signal presence of papillomas in PND patients. However, further refinement and validation in clinical trials are necessary to establish its clinical applicability. |
| first_indexed | 2024-03-08T03:54:57Z |
| format | Article |
| id | doaj.art-b575a479712847fdab0eb0adb614103f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:54:57Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b575a479712847fdab0eb0adb614103f2024-02-09T15:14:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01253181210.3390/ijms25031812The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple DischargeSeher Makineli0Menno R. Vriens1Arjen J. Witkamp2Paul J. van Diest3Cathy B. Moelans4Department of Surgical Oncology, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Surgical Oncology, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Surgical Oncology, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsDepartment of Pathology, University Medical Center Utrecht, 3584 CX Utrecht, The NetherlandsPatients with pathological nipple discharge (PND) often undergo local surgical procedures because standard radiologic imaging fails to identify the underlying cause. MicroRNA (MiRNA) expression analysis of nipple fluid holds potential for distinguishing between breast diseases. This study aimed to compare miRNA expression levels between nipple fluids from patients with PND to identify possible relevant miRNAs that could differentiate between intraductal papillomas and no abnormalities in the breast tissue. Nipple fluid samples from patients with PND without radiological and pathological suspicion for malignancy who underwent a ductoscopy procedure were analyzed. We used univariate and multivariate regression analyses to identify nipple fluid miRNAs differing between pathologically confirmed papillomas and breast tissue without abnormalities. A total of 27 nipple fluid samples from patients with PND were included for miRNA expression analysis. Out of the 22 miRNAs examined, only miR-145-5p was significantly differentially expressed (upregulated) in nipple fluid from patients with an intraductal papilloma compared to patients showing no breast abnormalities (OR 4.76, <i>p</i> = 0.046), with a diagnostic accuracy of 92%. miR-145-5p expression in nipple fluid differs for intraductal papillomas and breast tissue without abnormalities and, therefore, has potential as a diagnostic marker to signal presence of papillomas in PND patients. However, further refinement and validation in clinical trials are necessary to establish its clinical applicability.https://www.mdpi.com/1422-0067/25/3/1812miRNAbiomarkersnipple dischargeductoscopybreast cancer |
| spellingShingle | Seher Makineli Menno R. Vriens Arjen J. Witkamp Paul J. van Diest Cathy B. Moelans The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge International Journal of Molecular Sciences miRNA biomarkers nipple discharge ductoscopy breast cancer |
| title | The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge |
| title_full | The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge |
| title_fullStr | The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge |
| title_full_unstemmed | The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge |
| title_short | The Diagnostic Value of microRNA Expression Analysis in Detecting Intraductal Papillomas in Patients with Pathological Nipple Discharge |
| title_sort | diagnostic value of microrna expression analysis in detecting intraductal papillomas in patients with pathological nipple discharge |
| topic | miRNA biomarkers nipple discharge ductoscopy breast cancer |
| url | https://www.mdpi.com/1422-0067/25/3/1812 |
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