Towards an advanced cell-based <em>in vitro</em> glioma model system
The modulation of tumor growth and development <em>in vitro</em> has always been one of the key factors in the research of the malignant transformation, including gliomas, prevalent and most deadly cancers of the brain. Indeed, cellular and molecular biology research employing <em>...
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| Format: | Article |
| Language: | English |
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AIMS Press
2018-03-01
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| Series: | AIMS Genetics |
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| Online Access: | http://www.aimspress.com/article/10.3934/genet.2018.2.91/fulltext.html |
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| author | Valeriia Mikhailova Valeriia Gulaia Vladlena Tiasto Stanislav Rybtsov Margarita Yatsunskaya Alexander Kagansky |
| author_facet | Valeriia Mikhailova Valeriia Gulaia Vladlena Tiasto Stanislav Rybtsov Margarita Yatsunskaya Alexander Kagansky |
| author_sort | Valeriia Mikhailova |
| collection | DOAJ |
| description | The modulation of tumor growth and development <em>in vitro</em> has always been one of the key factors in the research of the malignant transformation, including gliomas, prevalent and most deadly cancers of the brain. Indeed, cellular and molecular biology research employing <em>in vitro</em> model cell-based systems have great potential to advance both the mechanistic understanding and the treatment of human glial tumors, as it facilitates not only the understanding of glioma biology and its regulatory mechanisms Additionally they promise to afford the screening of the putative anti-tumor agents and alternative treatment approaches in a personalized manner, i.e. by virtue of using the patient-derived tumor material for such tests. However, in order to become reliable and representative, glioma model systems need to move towards including most inherent cancer features such as local hypoxia, specific genetic aberrations, native tumor microenvironment, and the three-dimensional extracellular matrix.<br />
This review starts with a brief introduction on the general epidemiological and molecular characteristics of gliomas followed by an overview of the cell-based <em>in vitro</em> models currently used in glioma research. As a conclusion, we suggest approaches to move to innovative cell-based <em>in vitro</em> glioma models. We consider that main criteria for selecting these approaches should include the adequate resemblance to the key in vivo characteristics, robustness, cost-effectiveness and ease to use, as well as the amenability to high throughput handling to allow the standardized drug screening. |
| first_indexed | 2024-12-21T12:07:31Z |
| format | Article |
| id | doaj.art-b579a63737af45258248a9b01efddb95 |
| institution | Directory Open Access Journal |
| issn | 2377-1143 |
| language | English |
| last_indexed | 2024-12-21T12:07:31Z |
| publishDate | 2018-03-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Genetics |
| spelling | doaj.art-b579a63737af45258248a9b01efddb952022-12-21T19:04:41ZengAIMS PressAIMS Genetics2377-11432018-03-01529111210.3934/genet.2018.2.91Towards an advanced cell-based <em>in vitro</em> glioma model systemValeriia Mikhailova0Valeriia Gulaia1Vladlena Tiasto2Stanislav Rybtsov3Margarita Yatsunskaya4Alexander Kagansky51 Center for Genomic and Regenerative Medicine, School of Biomedicine, Far Eastern Federal University, Vladivostok, Russian Federation1 Center for Genomic and Regenerative Medicine, School of Biomedicine, Far Eastern Federal University, Vladivostok, Russian Federation1 Center for Genomic and Regenerative Medicine, School of Biomedicine, Far Eastern Federal University, Vladivostok, Russian Federation2 Scottish Centre for Regenerative Medicine of the University of Edinburgh, Edinburgh, United Kingdom3 Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS 159, Stoletij Vladivostoku Avenue, 690022, Vladivostok, Russian Federation1 Center for Genomic and Regenerative Medicine, School of Biomedicine, Far Eastern Federal University, Vladivostok, Russian FederationThe modulation of tumor growth and development <em>in vitro</em> has always been one of the key factors in the research of the malignant transformation, including gliomas, prevalent and most deadly cancers of the brain. Indeed, cellular and molecular biology research employing <em>in vitro</em> model cell-based systems have great potential to advance both the mechanistic understanding and the treatment of human glial tumors, as it facilitates not only the understanding of glioma biology and its regulatory mechanisms Additionally they promise to afford the screening of the putative anti-tumor agents and alternative treatment approaches in a personalized manner, i.e. by virtue of using the patient-derived tumor material for such tests. However, in order to become reliable and representative, glioma model systems need to move towards including most inherent cancer features such as local hypoxia, specific genetic aberrations, native tumor microenvironment, and the three-dimensional extracellular matrix.<br /> This review starts with a brief introduction on the general epidemiological and molecular characteristics of gliomas followed by an overview of the cell-based <em>in vitro</em> models currently used in glioma research. As a conclusion, we suggest approaches to move to innovative cell-based <em>in vitro</em> glioma models. We consider that main criteria for selecting these approaches should include the adequate resemblance to the key in vivo characteristics, robustness, cost-effectiveness and ease to use, as well as the amenability to high throughput handling to allow the standardized drug screening.http://www.aimspress.com/article/10.3934/genet.2018.2.91/fulltext.htmlbrain tumors| glioma| <em>in vitro</em> tumor model system| glioma model| neurospheres| glial cell lines| primary glial cell cultures| cancer stem cells |
| spellingShingle | Valeriia Mikhailova Valeriia Gulaia Vladlena Tiasto Stanislav Rybtsov Margarita Yatsunskaya Alexander Kagansky Towards an advanced cell-based <em>in vitro</em> glioma model system AIMS Genetics brain tumors| glioma| <em>in vitro</em> tumor model system| glioma model| neurospheres| glial cell lines| primary glial cell cultures| cancer stem cells |
| title | Towards an advanced cell-based <em>in vitro</em> glioma model system |
| title_full | Towards an advanced cell-based <em>in vitro</em> glioma model system |
| title_fullStr | Towards an advanced cell-based <em>in vitro</em> glioma model system |
| title_full_unstemmed | Towards an advanced cell-based <em>in vitro</em> glioma model system |
| title_short | Towards an advanced cell-based <em>in vitro</em> glioma model system |
| title_sort | towards an advanced cell based em in vitro em glioma model system |
| topic | brain tumors| glioma| <em>in vitro</em> tumor model system| glioma model| neurospheres| glial cell lines| primary glial cell cultures| cancer stem cells |
| url | http://www.aimspress.com/article/10.3934/genet.2018.2.91/fulltext.html |
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