Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd
Phytoremediation efficiency depends on the ability of plants to accumulate, translocate and resist high levels of metals without symptoms of toxicity. This study was conducted to evaluate the potential of grain amaranth for remediation of soils contaminated with Cd. Three grain amaranth varieties, “...
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MDPI AG
2020-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/572 |
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| author | Veronika Lancíková Marián Tomka Jana Žiarovská Ján Gažo Andrea Hricová |
| author_facet | Veronika Lancíková Marián Tomka Jana Žiarovská Ján Gažo Andrea Hricová |
| author_sort | Veronika Lancíková |
| collection | DOAJ |
| description | Phytoremediation efficiency depends on the ability of plants to accumulate, translocate and resist high levels of metals without symptoms of toxicity. This study was conducted to evaluate the potential of grain amaranth for remediation of soils contaminated with Cd. Three grain amaranth varieties, “Pribina” (<i>A. cruentus</i>), “Zobor” (<i>A. hypochondriacus x A. hybridus</i>) and Plainsman (<i>A. hypochondriacus x A. hybridus</i>) were tested under different level of Cd (0, 5, 10 and 15 mg/L) in a hydroponic experimental treatment. All could be classified as Cd excluders or Cd-hypertolerant varieties able to grow and accumulate significant amounts of Cd from the hydroponic solution, preferentially in the roots. Under the highest level of Cd exposure, qRT-PCR expression analysis of five stress-related genes was examined in above- and below-ground biomass. The results show that the Cd concentration significantly increased the mRNA level of chitinase 5 (<i>Chit 5</i>) in amaranth roots as the primary site of metal stress. The involvement of phytochelatin synthase (<i>PCS1</i>) in Cd detoxification is suggested. Based on our findings, we can conclude that variety “Pribina” is the most Cd-tolerant among three tested and can be expected to be used in the phytomanagement of Cd loaded soils as an effective phytostabiliser. |
| first_indexed | 2024-03-10T20:08:44Z |
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| language | English |
| last_indexed | 2024-03-10T20:08:44Z |
| publishDate | 2020-04-01 |
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| series | Plants |
| spelling | doaj.art-b57fe66de4f64d5aa0bdb043fe37a5242023-11-19T23:07:44ZengMDPI AGPlants2223-77472020-04-019557210.3390/plants9050572Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under CdVeronika Lancíková0Marián Tomka1Jana Žiarovská2Ján Gažo3Andrea Hricová4Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Nitra 95007, SlovakiaDepartment of Biochemistry, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra 94976, SlovakiaDepartment of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra 94976, SlovakiaDepartment of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Nitra 94976, SlovakiaInstitute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Nitra 95007, SlovakiaPhytoremediation efficiency depends on the ability of plants to accumulate, translocate and resist high levels of metals without symptoms of toxicity. This study was conducted to evaluate the potential of grain amaranth for remediation of soils contaminated with Cd. Three grain amaranth varieties, “Pribina” (<i>A. cruentus</i>), “Zobor” (<i>A. hypochondriacus x A. hybridus</i>) and Plainsman (<i>A. hypochondriacus x A. hybridus</i>) were tested under different level of Cd (0, 5, 10 and 15 mg/L) in a hydroponic experimental treatment. All could be classified as Cd excluders or Cd-hypertolerant varieties able to grow and accumulate significant amounts of Cd from the hydroponic solution, preferentially in the roots. Under the highest level of Cd exposure, qRT-PCR expression analysis of five stress-related genes was examined in above- and below-ground biomass. The results show that the Cd concentration significantly increased the mRNA level of chitinase 5 (<i>Chit 5</i>) in amaranth roots as the primary site of metal stress. The involvement of phytochelatin synthase (<i>PCS1</i>) in Cd detoxification is suggested. Based on our findings, we can conclude that variety “Pribina” is the most Cd-tolerant among three tested and can be expected to be used in the phytomanagement of Cd loaded soils as an effective phytostabiliser.https://www.mdpi.com/2223-7747/9/5/572cadmium stressgrain amaranthstress-related genesphytoremediation potential |
| spellingShingle | Veronika Lancíková Marián Tomka Jana Žiarovská Ján Gažo Andrea Hricová Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd Plants cadmium stress grain amaranth stress-related genes phytoremediation potential |
| title | Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd |
| title_full | Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd |
| title_fullStr | Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd |
| title_full_unstemmed | Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd |
| title_short | Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd |
| title_sort | morphological responses and gene expression of grain amaranth i amaranthus i spp growing under cd |
| topic | cadmium stress grain amaranth stress-related genes phytoremediation potential |
| url | https://www.mdpi.com/2223-7747/9/5/572 |
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