A glucose-starvation response regulates the diffusion of macromolecules
The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of buddi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/09376 |
| _version_ | 1811235866399473664 |
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| author | Ryan P Joyner Jeffrey H Tang Jonne Helenius Elisa Dultz Christiane Brune Liam J Holt Sebastien Huet Daniel J Müller Karsten Weis |
| author_facet | Ryan P Joyner Jeffrey H Tang Jonne Helenius Elisa Dultz Christiane Brune Liam J Holt Sebastien Huet Daniel J Müller Karsten Weis |
| author_sort | Ryan P Joyner |
| collection | DOAJ |
| description | The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes a startling transition upon glucose starvation in which macromolecular mobility is dramatically restricted, reducing the movement of both chromatin in the nucleus and mRNPs in the cytoplasm. This confinement cannot be explained by an ATP decrease or the physiological drop in intracellular pH. Rather, our results suggest that the regulation of diffusional mobility is induced by a reduction in cell volume and subsequent increase in molecular crowding which severely alters the biophysical properties of the intracellular environment. A similar response can be observed in fission yeast and bacteria. This reveals a novel mechanism by which cells globally alter their properties to establish a unique homeostasis during starvation. |
| first_indexed | 2024-04-12T11:59:26Z |
| format | Article |
| id | doaj.art-b5816db166aa46e5a65685b7f4e6142a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T11:59:26Z |
| publishDate | 2016-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b5816db166aa46e5a65685b7f4e6142a2022-12-22T03:33:55ZengeLife Sciences Publications LtdeLife2050-084X2016-03-01510.7554/eLife.09376A glucose-starvation response regulates the diffusion of macromoleculesRyan P Joyner0Jeffrey H Tang1Jonne Helenius2Elisa Dultz3Christiane Brune4Liam J Holt5https://orcid.org/0000-0002-4002-0861Sebastien Huet6Daniel J Müller7Karsten Weis8https://orcid.org/0000-0001-7224-925XDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesInstitute of Biochemistry, Department of Biology, ETH Zurich, Zürich, SwitzerlandDepartment of Biosystems Science and Engineering, ETH Zurich, Zürich, SwitzerlandDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesInstitute for Systems Genetics, New York University School of Medicine, New York, United StatesCNRS, UMR 6290, Institut Génétique et Développement, University of Rennes, Rennes, FranceDepartment of Biosystems Science and Engineering, ETH Zurich, Zürich, SwitzerlandDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Institute of Biochemistry, Department of Biology, ETH Zurich, Zürich, SwitzerlandThe organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes a startling transition upon glucose starvation in which macromolecular mobility is dramatically restricted, reducing the movement of both chromatin in the nucleus and mRNPs in the cytoplasm. This confinement cannot be explained by an ATP decrease or the physiological drop in intracellular pH. Rather, our results suggest that the regulation of diffusional mobility is induced by a reduction in cell volume and subsequent increase in molecular crowding which severely alters the biophysical properties of the intracellular environment. A similar response can be observed in fission yeast and bacteria. This reveals a novel mechanism by which cells globally alter their properties to establish a unique homeostasis during starvation.https://elifesciences.org/articles/09376crowdingdiffusionstarvation |
| spellingShingle | Ryan P Joyner Jeffrey H Tang Jonne Helenius Elisa Dultz Christiane Brune Liam J Holt Sebastien Huet Daniel J Müller Karsten Weis A glucose-starvation response regulates the diffusion of macromolecules eLife crowding diffusion starvation |
| title | A glucose-starvation response regulates the diffusion of macromolecules |
| title_full | A glucose-starvation response regulates the diffusion of macromolecules |
| title_fullStr | A glucose-starvation response regulates the diffusion of macromolecules |
| title_full_unstemmed | A glucose-starvation response regulates the diffusion of macromolecules |
| title_short | A glucose-starvation response regulates the diffusion of macromolecules |
| title_sort | glucose starvation response regulates the diffusion of macromolecules |
| topic | crowding diffusion starvation |
| url | https://elifesciences.org/articles/09376 |
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