The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis
Background Hepatic stellate cells (HSCs) are the central players interacting with multiple cell types in liver fibrosis. The crosstalk between HSCs and macrophages has recently become clearer. Irisin, an exercise-responsive myokine, was known to have a potentially protective role in liver and renal...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Endocrine Society
2022-08-01
|
| Series: | Endocrinology and Metabolism |
| Subjects: | |
| Online Access: | http://www.e-enm.org/upload/pdf/enm-2022-1412.pdf |
| _version_ | 1817986697943580672 |
|---|---|
| author | Dinh Vinh Do So Young Park Giang Thi Nguyen Dae Hee Choi Eun-Hee Cho |
| author_facet | Dinh Vinh Do So Young Park Giang Thi Nguyen Dae Hee Choi Eun-Hee Cho |
| author_sort | Dinh Vinh Do |
| collection | DOAJ |
| description | Background Hepatic stellate cells (HSCs) are the central players interacting with multiple cell types in liver fibrosis. The crosstalk between HSCs and macrophages has recently become clearer. Irisin, an exercise-responsive myokine, was known to have a potentially protective role in liver and renal fibrosis, especially in connection with stellate cells. This study investigated the effects of irisin on the interaction between HSCs and macrophages. Methods Tamm-Horsfall protein-1 (THP-1) human monocytes were differentiated into macrophages, polarized into the inflammatory M1 phenotype with lipopolysaccharide. Lieming Xu-2 (LX-2) cells, human HSCs, were treated with conditioned media (CM) from M1 macrophages, with or without recombinant irisin. HSCs responses to CM from M1 macrophages were evaluated regarding activation, proliferation, wound healing, trans-well migration, contractility, and related signaling pathway. Results CM from M1 macrophages significantly promoted HSC proliferation, wound healing, transwell migration, and contractility, but not activation of HSCs. Irisin co-treatment attenuated these responses of HSCs to CM. However, CM and irisin treatment did not induce any changes in HSC activation. Further, irisin co-treatment alleviated CM-induced increase of phopho-protein kinase B (pAKT), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinases-1 (TIMP-1). Conclusion These findings suggested that irisin may play a protective role in the pathogenesis of liver fibrosis, especially when working in the crosstalk between HSCs and macrophages. |
| first_indexed | 2024-04-14T00:12:36Z |
| format | Article |
| id | doaj.art-b586d6ace7714d2c9a5cff40e43939f5 |
| institution | Directory Open Access Journal |
| issn | 2093-596X 2093-5978 |
| language | English |
| last_indexed | 2024-04-14T00:12:36Z |
| publishDate | 2022-08-01 |
| publisher | Korean Endocrine Society |
| record_format | Article |
| series | Endocrinology and Metabolism |
| spelling | doaj.art-b586d6ace7714d2c9a5cff40e43939f52022-12-22T02:23:16ZengKorean Endocrine SocietyEndocrinology and Metabolism2093-596X2093-59782022-08-0137462062910.3803/EnM.2022.14122318The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver FibrosisDinh Vinh Do0So Young Park1Giang Thi Nguyen2Dae Hee Choi3Eun-Hee Cho Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, Korea Department of Internal Medicine, Kangwon National University School of Medicine, Chuncheon, KoreaBackground Hepatic stellate cells (HSCs) are the central players interacting with multiple cell types in liver fibrosis. The crosstalk between HSCs and macrophages has recently become clearer. Irisin, an exercise-responsive myokine, was known to have a potentially protective role in liver and renal fibrosis, especially in connection with stellate cells. This study investigated the effects of irisin on the interaction between HSCs and macrophages. Methods Tamm-Horsfall protein-1 (THP-1) human monocytes were differentiated into macrophages, polarized into the inflammatory M1 phenotype with lipopolysaccharide. Lieming Xu-2 (LX-2) cells, human HSCs, were treated with conditioned media (CM) from M1 macrophages, with or without recombinant irisin. HSCs responses to CM from M1 macrophages were evaluated regarding activation, proliferation, wound healing, trans-well migration, contractility, and related signaling pathway. Results CM from M1 macrophages significantly promoted HSC proliferation, wound healing, transwell migration, and contractility, but not activation of HSCs. Irisin co-treatment attenuated these responses of HSCs to CM. However, CM and irisin treatment did not induce any changes in HSC activation. Further, irisin co-treatment alleviated CM-induced increase of phopho-protein kinase B (pAKT), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinases-1 (TIMP-1). Conclusion These findings suggested that irisin may play a protective role in the pathogenesis of liver fibrosis, especially when working in the crosstalk between HSCs and macrophages.http://www.e-enm.org/upload/pdf/enm-2022-1412.pdfirisinhepatic stellate cellsmacrophagesliver cirrhosis |
| spellingShingle | Dinh Vinh Do So Young Park Giang Thi Nguyen Dae Hee Choi Eun-Hee Cho The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis Endocrinology and Metabolism irisin hepatic stellate cells macrophages liver cirrhosis |
| title | The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis |
| title_full | The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis |
| title_fullStr | The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis |
| title_full_unstemmed | The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis |
| title_short | The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis |
| title_sort | effects of irisin on the interaction between hepatic stellate cell and macrophage in liver fibrosis |
| topic | irisin hepatic stellate cells macrophages liver cirrhosis |
| url | http://www.e-enm.org/upload/pdf/enm-2022-1412.pdf |
| work_keys_str_mv | AT dinhvinhdo theeffectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT soyoungpark theeffectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT giangthinguyen theeffectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT daeheechoi theeffectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT eunheecho theeffectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT dinhvinhdo effectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT soyoungpark effectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT giangthinguyen effectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT daeheechoi effectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis AT eunheecho effectsofirisinontheinteractionbetweenhepaticstellatecellandmacrophageinliverfibrosis |