Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms
(1) Background: The effectiveness of chitosan to improve the action of antimicrobial compounds against planktonic bacteria and young biofilms has been widely investigated in Dentistry, where the biofilm lifecycle is a determining factor for the success of antibacterial treatment. In the present stud...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/9/1808 |
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| author | Erika R. H. Kawakita Ana Carolina S. Ré Maria Paula G. Peixoto Maíra P. Ferreira Antônio P. Ricomini-Filho Osvaldo Freitas Carolina P. Aires |
| author_facet | Erika R. H. Kawakita Ana Carolina S. Ré Maria Paula G. Peixoto Maíra P. Ferreira Antônio P. Ricomini-Filho Osvaldo Freitas Carolina P. Aires |
| author_sort | Erika R. H. Kawakita |
| collection | DOAJ |
| description | (1) Background: The effectiveness of chitosan to improve the action of antimicrobial compounds against planktonic bacteria and young biofilms has been widely investigated in Dentistry, where the biofilm lifecycle is a determining factor for the success of antibacterial treatment. In the present study, mature <i>Streptococcus mutans</i> biofilms were treated with chitosan dispersion (CD) or chitosan microparticles (CM). (2) Methods: CD at 0.25% and 1% were characterized by texture analysis, while CD at 2% was spray-dried to form CM, which were characterized with respect to particle size distribution, zeta potential, and morphology. After determining the minimum inhibitory and bactericidal concentrations, <i>S. mutans</i> biofilms were grown on glass slides exposed 8×/day to 10% sucrose and 2×/day to CD or CM at 0.25% and 1%. Biofilm viability and acidogenicity were determined, using appropriate control groups for each experiment. (3) Results: CD had high viscosity and CM were spherical, with narrow size distribution and positive zeta potential. CM affected bacterial viability and acidogenicity in mature <i>S. mutans</i> biofilms more strongly than CD, especially at 1%. (4) Conclusions: Both chitosan forms exerted antimicrobial effect against mature <i>S. mutans</i> biofilms. CM at 1% can reduce bacterial viability and acidogenicity more effectively than CD at 1%, and thereby be more effective to control the growth of mature biofilms in vitro. |
| first_indexed | 2024-04-13T07:35:07Z |
| format | Article |
| id | doaj.art-b58df97aeb0e4bdcb77ea3b8b1087472 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T07:35:07Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b58df97aeb0e4bdcb77ea3b8b10874722022-12-22T02:56:12ZengMDPI AGMolecules1420-30492019-05-01249180810.3390/molecules24091808molecules24091808Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> BiofilmsErika R. H. Kawakita0Ana Carolina S. Ré1Maria Paula G. Peixoto2Maíra P. Ferreira3Antônio P. Ricomini-Filho4Osvaldo Freitas5Carolina P. Aires6Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, BrazilDepartment of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, BrazilDepartment of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, BrazilDepartment of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, BrazilDepartment of Physiological Sciences, Piracicaba Dental School, University of Campinas, Piracicaba 13414-903, São Paulo, BrazilDepartment of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, BrazilDepartment of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14040-903, São Paulo, Brazil(1) Background: The effectiveness of chitosan to improve the action of antimicrobial compounds against planktonic bacteria and young biofilms has been widely investigated in Dentistry, where the biofilm lifecycle is a determining factor for the success of antibacterial treatment. In the present study, mature <i>Streptococcus mutans</i> biofilms were treated with chitosan dispersion (CD) or chitosan microparticles (CM). (2) Methods: CD at 0.25% and 1% were characterized by texture analysis, while CD at 2% was spray-dried to form CM, which were characterized with respect to particle size distribution, zeta potential, and morphology. After determining the minimum inhibitory and bactericidal concentrations, <i>S. mutans</i> biofilms were grown on glass slides exposed 8×/day to 10% sucrose and 2×/day to CD or CM at 0.25% and 1%. Biofilm viability and acidogenicity were determined, using appropriate control groups for each experiment. (3) Results: CD had high viscosity and CM were spherical, with narrow size distribution and positive zeta potential. CM affected bacterial viability and acidogenicity in mature <i>S. mutans</i> biofilms more strongly than CD, especially at 1%. (4) Conclusions: Both chitosan forms exerted antimicrobial effect against mature <i>S. mutans</i> biofilms. CM at 1% can reduce bacterial viability and acidogenicity more effectively than CD at 1%, and thereby be more effective to control the growth of mature biofilms in vitro.https://www.mdpi.com/1420-3049/24/9/1808polysaccharidepharmaceutical applicationsstructured biofilmchitosan |
| spellingShingle | Erika R. H. Kawakita Ana Carolina S. Ré Maria Paula G. Peixoto Maíra P. Ferreira Antônio P. Ricomini-Filho Osvaldo Freitas Carolina P. Aires Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms Molecules polysaccharide pharmaceutical applications structured biofilm chitosan |
| title | Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms |
| title_full | Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms |
| title_fullStr | Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms |
| title_full_unstemmed | Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms |
| title_short | Effect of Chitosan Dispersion and Microparticles on Older <i>Streptococcus mutans</i> Biofilms |
| title_sort | effect of chitosan dispersion and microparticles on older i streptococcus mutans i biofilms |
| topic | polysaccharide pharmaceutical applications structured biofilm chitosan |
| url | https://www.mdpi.com/1420-3049/24/9/1808 |
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