Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus
Systemic lupus erythematosus (SLE) is a debilitating autoimmune disorder characterized by unknown pathogenesis and heterogeneous clinical manifestations. The current existing serum biomarkers for SLE have limited sensitivity or specificity, making early and precise diagnosis difficult. Here, we iden...
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| Language: | English |
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Elsevier
2023-07-01
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| Series: | Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095809923000930 |
| _version_ | 1827805995131207680 |
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| author | Hudan Pan Jingrong Wang Yong Liang Canjian Wang Ruimin Tian Hua Ye Xiao Zhang Yuanhao Wu Miao Shao Ruijun Zhang Yao Xiao Zhi Li Guangfeng Zhang Hua Zhou Yilin Wang Xiaoshuang Wang Zhanguo Li Wei Liu Liang Liu |
| author_facet | Hudan Pan Jingrong Wang Yong Liang Canjian Wang Ruimin Tian Hua Ye Xiao Zhang Yuanhao Wu Miao Shao Ruijun Zhang Yao Xiao Zhi Li Guangfeng Zhang Hua Zhou Yilin Wang Xiaoshuang Wang Zhanguo Li Wei Liu Liang Liu |
| author_sort | Hudan Pan |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a debilitating autoimmune disorder characterized by unknown pathogenesis and heterogeneous clinical manifestations. The current existing serum biomarkers for SLE have limited sensitivity or specificity, making early and precise diagnosis difficult. Here, we identified two N-glycans on serum immunoglobulin G (IgG) as excellent diagnostic biomarkers for SLE based on in-depth glycomic analyses of 389 SLE patients and 304 healthy controls. These two N-glycan biomarkers are specific for diagnosing SLE, as no significant changes in these biomarkers were observed in other systemic autoimmune diseases that are easily confused with SLE, such as rheumatoid arthritis, primary Sjögren’s syndrome, or systemic sclerosis. Notably, the two N-glycan biomarkers proved to be autoantibody-independent and all-stage patient suitable. The two N-glycan biomarkers are demonstrated to be located on the Fc region based on fragment-specific glycan analysis and glycopeptide analysis, suggesting their close correlation with disease activity. Enzyme analyses revealed dysregulation of a series of glycotransferases in SLE, which might be responsible for the observed glycan alteration. Our findings provide insights into efficient population screening based on serum IgG glycosylation and potential new pathogenic factors of SLE. |
| first_indexed | 2024-03-11T21:35:07Z |
| format | Article |
| id | doaj.art-b58e0bba2dca4b25aeb754289be3f32c |
| institution | Directory Open Access Journal |
| issn | 2095-8099 |
| language | English |
| last_indexed | 2024-03-11T21:35:07Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Engineering |
| spelling | doaj.art-b58e0bba2dca4b25aeb754289be3f32c2023-09-27T04:42:45ZengElsevierEngineering2095-80992023-07-01268998Serum IgG Glycan Hallmarks of Systemic Lupus ErythematosusHudan Pan0Jingrong Wang1Yong Liang2Canjian Wang3Ruimin Tian4Hua Ye5Xiao Zhang6Yuanhao Wu7Miao Shao8Ruijun Zhang9Yao Xiao10Zhi Li11Guangfeng Zhang12Hua Zhou13Yilin Wang14Xiaoshuang Wang15Zhanguo Li16Wei Liu17Liang Liu18State Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China; Guangdong–Hong Kong–Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou 510000, ChinaState Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, ChinaPeng Cheng Laboratory, Shenzhen 518055, ChinaNational Engineering Research Center of Chinese Medicine Solid Preparation Manufacturing Technology, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaState Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China; State Key Laboratory of Quality Research in Chinese Medicine & Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao 999078, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing 100044, ChinaGuangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, ChinaThe First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing 100044, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing 100044, ChinaState Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, ChinaZhuhai Hospital of Integrated Traditional Chinese and Western Medicine, Zhuhai 519020, ChinaGuangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, ChinaState Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, ChinaState Key Laboratory of Quality Research in Chinese Medicine & Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao 999078, China; Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine, Zhuhai 519020, ChinaThe First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaDepartment of Rheumatology and Immunology, Peking University People’s Hospital, Beijing 100044, China; Corresponding authors.The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; Corresponding authors.State Key Laboratory of Traditional Chinese Medicine Syndrome, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China; Guangdong–Hong Kong–Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou 510000, China; State Key Laboratory of Quality Research in Chinese Medicine & Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao 999078, China; Corresponding authors.Systemic lupus erythematosus (SLE) is a debilitating autoimmune disorder characterized by unknown pathogenesis and heterogeneous clinical manifestations. The current existing serum biomarkers for SLE have limited sensitivity or specificity, making early and precise diagnosis difficult. Here, we identified two N-glycans on serum immunoglobulin G (IgG) as excellent diagnostic biomarkers for SLE based on in-depth glycomic analyses of 389 SLE patients and 304 healthy controls. These two N-glycan biomarkers are specific for diagnosing SLE, as no significant changes in these biomarkers were observed in other systemic autoimmune diseases that are easily confused with SLE, such as rheumatoid arthritis, primary Sjögren’s syndrome, or systemic sclerosis. Notably, the two N-glycan biomarkers proved to be autoantibody-independent and all-stage patient suitable. The two N-glycan biomarkers are demonstrated to be located on the Fc region based on fragment-specific glycan analysis and glycopeptide analysis, suggesting their close correlation with disease activity. Enzyme analyses revealed dysregulation of a series of glycotransferases in SLE, which might be responsible for the observed glycan alteration. Our findings provide insights into efficient population screening based on serum IgG glycosylation and potential new pathogenic factors of SLE.http://www.sciencedirect.com/science/article/pii/S2095809923000930Systemic lupus erythematosusN-glycansDiagnostic indicators |
| spellingShingle | Hudan Pan Jingrong Wang Yong Liang Canjian Wang Ruimin Tian Hua Ye Xiao Zhang Yuanhao Wu Miao Shao Ruijun Zhang Yao Xiao Zhi Li Guangfeng Zhang Hua Zhou Yilin Wang Xiaoshuang Wang Zhanguo Li Wei Liu Liang Liu Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus Engineering Systemic lupus erythematosus N-glycans Diagnostic indicators |
| title | Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus |
| title_full | Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus |
| title_fullStr | Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus |
| title_full_unstemmed | Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus |
| title_short | Serum IgG Glycan Hallmarks of Systemic Lupus Erythematosus |
| title_sort | serum igg glycan hallmarks of systemic lupus erythematosus |
| topic | Systemic lupus erythematosus N-glycans Diagnostic indicators |
| url | http://www.sciencedirect.com/science/article/pii/S2095809923000930 |
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