Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach
Tetrastigma leucostaphylum (Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of T. leucostaphylum (METL). In vivo analgesic and anti-inflammatory researches have been implemented...
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| Format: | Article |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2020-12-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=109652 |
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| author | Sajib Rudra Md. Solaman Uddin Sawon Nazim Uddin Emon Safaet Alam Syed Mohammed Tareq Mohammad Nazmul Islam Mir Md. Rokib Uddin Abdullah Md Sazid Chowdhury Abu Nazer Hasbe Mohammad Shakil Shahenur Alam Sakib Mohammed Aktar Sayeed |
| author_facet | Sajib Rudra Md. Solaman Uddin Sawon Nazim Uddin Emon Safaet Alam Syed Mohammed Tareq Mohammad Nazmul Islam Mir Md. Rokib Uddin Abdullah Md Sazid Chowdhury Abu Nazer Hasbe Mohammad Shakil Shahenur Alam Sakib Mohammed Aktar Sayeed |
| author_sort | Sajib Rudra |
| collection | DOAJ |
| description | Tetrastigma leucostaphylum (Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of T. leucostaphylum (METL). In vivo analgesic and anti-inflammatory researches have been implemented by using acetic acid-induced writhing and formalin-induced paw licking test protocols in mice. Furthermore, in vitro thrombolytic and anthelmintic studies have been performed by following the blood clot lysis method and nematode mortality measurement method. In the in vivo study, METL did not minimize the acetic acid-induced writhes prominently but significantly attenuate both the peripheral and inflammatory pain in mice in a dose-dependent manner. In early and late phase, METL 400 (mg/kg, b.w; p.o) showed 39.63 % and 48.73 % paw licking inhibition. Again, METL (100 µL) reflected 56.62 % clot lysis in thrombolytic research. Besides, METL causes the death of nematodes in a dose-dependent manner. The bioassay of the methanol extract of T. leucostaphylum justified the analgesic, anti-inflammatory, thrombolytic and anthelmintic activities of the crude extract and finally suggests the test extract as a wellspring of anti-inflammatory, thrombolytic and anthelmintic agents as a crude drug source. [ J Adv Biotechnol Exp Ther 2020; 3(3.000): 216-224] |
| first_indexed | 2024-12-13T16:12:02Z |
| format | Article |
| id | doaj.art-b58f0dc8e1904cc18dc3ce9af7cebf0e |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-13T16:12:02Z |
| publishDate | 2020-12-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-b58f0dc8e1904cc18dc3ce9af7cebf0e2022-12-21T23:38:55ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602020-12-013321622410.5455/jabet.2020.d127109652Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approachSajib Rudra0Md. Solaman Uddin Sawon1Nazim Uddin Emon2Safaet Alam3Syed Mohammed Tareq4Mohammad Nazmul Islam5Mir Md. Rokib Uddin6Abdullah Md Sazid Chowdhury7Abu Nazer Hasbe8Mohammad Shakil9Shahenur Alam Sakib10Mohammed Aktar Sayeed11Department of Botany, Faculty of Biological Science, University of Chittagong, Chittagong - 4331, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Botany, Faculty of Biological Science, University of Chittagong, Chittagong - 4331, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, Bangladesh Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong - 4318, BangladeshTetrastigma leucostaphylum (Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of T. leucostaphylum (METL). In vivo analgesic and anti-inflammatory researches have been implemented by using acetic acid-induced writhing and formalin-induced paw licking test protocols in mice. Furthermore, in vitro thrombolytic and anthelmintic studies have been performed by following the blood clot lysis method and nematode mortality measurement method. In the in vivo study, METL did not minimize the acetic acid-induced writhes prominently but significantly attenuate both the peripheral and inflammatory pain in mice in a dose-dependent manner. In early and late phase, METL 400 (mg/kg, b.w; p.o) showed 39.63 % and 48.73 % paw licking inhibition. Again, METL (100 µL) reflected 56.62 % clot lysis in thrombolytic research. Besides, METL causes the death of nematodes in a dose-dependent manner. The bioassay of the methanol extract of T. leucostaphylum justified the analgesic, anti-inflammatory, thrombolytic and anthelmintic activities of the crude extract and finally suggests the test extract as a wellspring of anti-inflammatory, thrombolytic and anthelmintic agents as a crude drug source. [ J Adv Biotechnol Exp Ther 2020; 3(3.000): 216-224]http://www.ejmanager.com/fulltextpdf.php?mno=109652tetrastigma leucostaphylum; writhing; formalin; clot lysis; tubifex tubifex |
| spellingShingle | Sajib Rudra Md. Solaman Uddin Sawon Nazim Uddin Emon Safaet Alam Syed Mohammed Tareq Mohammad Nazmul Islam Mir Md. Rokib Uddin Abdullah Md Sazid Chowdhury Abu Nazer Hasbe Mohammad Shakil Shahenur Alam Sakib Mohammed Aktar Sayeed Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach Journal of Advanced Biotechnology and Experimental Therapeutics tetrastigma leucostaphylum; writhing; formalin; clot lysis; tubifex tubifex |
| title | Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach |
| title_full | Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach |
| title_fullStr | Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach |
| title_full_unstemmed | Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach |
| title_short | Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae) : In vivo and in vitro approach |
| title_sort | biological investigations of the methanol extract of tetrastigma leucostaphylum dennst alston ex mabb vitaceae in vivo and in vitro approach |
| topic | tetrastigma leucostaphylum; writhing; formalin; clot lysis; tubifex tubifex |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=109652 |
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