Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia
A Pratylenchus species identified during a survey of Pratylenchus quasitereoides incidence at four locations of the grainbelt of Western Australia is described. Morphological and morphometric features indicated the previously undescribed morphotypes in nematode mixtures encountered were conspecific...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-01-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.21307/jofnem-2019-046 |
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| author | Begum Farhana Fosu-Nyarko John Sharma Shashi Macleod Bill Collins Sarah Jones Michael G. K. |
| author_facet | Begum Farhana Fosu-Nyarko John Sharma Shashi Macleod Bill Collins Sarah Jones Michael G. K. |
| author_sort | Begum Farhana |
| collection | DOAJ |
| description | A Pratylenchus species identified during a survey of Pratylenchus quasitereoides incidence at four locations of the grainbelt of Western Australia is described. Morphological and morphometric features indicated the previously undescribed morphotypes in nematode mixtures encountered were conspecific to P. curvicauda, and were clearly distinguishable from nine common Pratylenchus spp. Typical features of P. curvicauda were its body length (415–540 µm), which was curved to a c-shaped with a maximum body diameter of 20 µm, and the nature of its tail; 34 µm long, 2.8 µm wide at the anus and a typical ventrally arcuate with a round terminus. Sequenced for the first time, the sequences of the partial 18S-ITS1-5.8S-ITS2-partial 28S (80 clones, 14 individual nematodes) and the 28S-D3 (17 clones) regions of the rDNA of P. curvicauda had overall mean distances of 0.013 and 0.085, respectively. Phylogenetic analyses with sequences of both segments of the rDNA clearly showed the P. curvicauda isolates as monophyletic, distinct from ca 40 Pratylenchus species. Notably, it was distinct from Pratylenchus species present in Australia including P. quasitereoides and a Western Australia isolate of P. thornei. Further research into the biology of P. curvicauda is needed to facilitate development of strategies for its management, if it is an important pest. |
| first_indexed | 2024-04-10T05:38:06Z |
| format | Article |
| id | doaj.art-b58f6b24a9f9498f8e214f6cdda969fc |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-10T05:38:06Z |
| publishDate | 2019-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-b58f6b24a9f9498f8e214f6cdda969fc2023-03-06T16:52:20ZengSciendoJournal of Nematology2640-396X2019-01-0151111510.21307/jofnem-2019-046Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western AustraliaBegum Farhana0Fosu-Nyarko John1Sharma Shashi2Macleod Bill3Collins Sarah4Jones Michael G. K.5Plant Biotechnology Research Group, WA State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, 6150, AustraliaPlant Biotechnology Research Group, WA State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, 6150, AustraliaDepartment of Primary Industries and Regional Development, South Perth, WA, 6151, AustraliaDepartment of Primary Industries and Regional Development, South Perth, WA, 6151, AustraliaDepartment of Primary Industries and Regional Development, South Perth, WA, 6151, AustraliaPlant Biotechnology Research Group, WA State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, 6150, AustraliaA Pratylenchus species identified during a survey of Pratylenchus quasitereoides incidence at four locations of the grainbelt of Western Australia is described. Morphological and morphometric features indicated the previously undescribed morphotypes in nematode mixtures encountered were conspecific to P. curvicauda, and were clearly distinguishable from nine common Pratylenchus spp. Typical features of P. curvicauda were its body length (415–540 µm), which was curved to a c-shaped with a maximum body diameter of 20 µm, and the nature of its tail; 34 µm long, 2.8 µm wide at the anus and a typical ventrally arcuate with a round terminus. Sequenced for the first time, the sequences of the partial 18S-ITS1-5.8S-ITS2-partial 28S (80 clones, 14 individual nematodes) and the 28S-D3 (17 clones) regions of the rDNA of P. curvicauda had overall mean distances of 0.013 and 0.085, respectively. Phylogenetic analyses with sequences of both segments of the rDNA clearly showed the P. curvicauda isolates as monophyletic, distinct from ca 40 Pratylenchus species. Notably, it was distinct from Pratylenchus species present in Australia including P. quasitereoides and a Western Australia isolate of P. thornei. Further research into the biology of P. curvicauda is needed to facilitate development of strategies for its management, if it is an important pest.https://doi.org/10.21307/jofnem-2019-046internal transcribed spacer (its)molecular phylogenymorphologynematode classificationpratylenchus spppratylenchus curvicaudaroot lesion nematodesscanning electron microscopy (sem)taxonomy |
| spellingShingle | Begum Farhana Fosu-Nyarko John Sharma Shashi Macleod Bill Collins Sarah Jones Michael G. K. Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia Journal of Nematology internal transcribed spacer (its) molecular phylogeny morphology nematode classification pratylenchus spp pratylenchus curvicauda root lesion nematodes scanning electron microscopy (sem) taxonomy |
| title | Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia |
| title_full | Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia |
| title_fullStr | Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia |
| title_full_unstemmed | Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia |
| title_short | Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia |
| title_sort | serendipitous identification of pratylenchus curvicauda from the grainbelt of western australia |
| topic | internal transcribed spacer (its) molecular phylogeny morphology nematode classification pratylenchus spp pratylenchus curvicauda root lesion nematodes scanning electron microscopy (sem) taxonomy |
| url | https://doi.org/10.21307/jofnem-2019-046 |
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