A Comprehensive Analysis of Cytokine Network in Centenarians
Cytokines have been investigated extensively in elderly people, with conflicting results. We performed a comprehensive analysis of the plasma levels of 62 cytokines and growth factors involved in the regulation of the immune system, in healthy centenarians, and middle-aged controls. We confirmed the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2719 |
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| author | Marcello Pinti Lara Gibellini Domenico Lo Tartaro Sara De Biasi Milena Nasi Rebecca Borella Lucia Fidanza Anita Neroni Leonarda Troiano Claudio Franceschi Andrea Cossarizza |
| author_facet | Marcello Pinti Lara Gibellini Domenico Lo Tartaro Sara De Biasi Milena Nasi Rebecca Borella Lucia Fidanza Anita Neroni Leonarda Troiano Claudio Franceschi Andrea Cossarizza |
| author_sort | Marcello Pinti |
| collection | DOAJ |
| description | Cytokines have been investigated extensively in elderly people, with conflicting results. We performed a comprehensive analysis of the plasma levels of 62 cytokines and growth factors involved in the regulation of the immune system, in healthy centenarians, and middle-aged controls. We confirmed the previously observed increase in the levels of several pro-inflammatory cytokines, such as TNF-α and IL-6, and found that several other cytokines, directly or indirectly involved in inflammation (such as IFN-α, IL-23, CCL-5), were present at higher levels in centenarians. We did not observe any increase in the levels of anti-inflammatory cytokines, with the notable exception of the Th2-shifting cytokine IL-19. No relevant difference was observed in cytokines regulating T cell immunity. Several growth factors having a role in regulating immunity, such as G-CSF, GM-CSF, EGF, and VEGF, were upregulated in centenarians, too. Principal component analysis of the cytokine dataset showed that pro and anti-inflammatory cytokines were the variables that contributed the most to the variability of the data we observed. |
| first_indexed | 2024-03-11T09:41:09Z |
| format | Article |
| id | doaj.art-b5909b41e78d43e8a7917609ac34546a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:41:09Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b5909b41e78d43e8a7917609ac34546a2023-11-16T17:01:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243271910.3390/ijms24032719A Comprehensive Analysis of Cytokine Network in CentenariansMarcello Pinti0Lara Gibellini1Domenico Lo Tartaro2Sara De Biasi3Milena Nasi4Rebecca Borella5Lucia Fidanza6Anita Neroni7Leonarda Troiano8Claudio Franceschi9Andrea Cossarizza10Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyPrevention and Protection Service, University of Modena and Reggio Emilia, 41121 Modena, ItalyDepartment of Medical and Surgical Sciences, Alma Mater Studiorum University of Bologna, 40126 Bologna, ItalyDepartment of Medical and Surgical Sciences of Children and Adults, University of Modena and Reggio Emilia, 41124 Modena, ItalyCytokines have been investigated extensively in elderly people, with conflicting results. We performed a comprehensive analysis of the plasma levels of 62 cytokines and growth factors involved in the regulation of the immune system, in healthy centenarians, and middle-aged controls. We confirmed the previously observed increase in the levels of several pro-inflammatory cytokines, such as TNF-α and IL-6, and found that several other cytokines, directly or indirectly involved in inflammation (such as IFN-α, IL-23, CCL-5), were present at higher levels in centenarians. We did not observe any increase in the levels of anti-inflammatory cytokines, with the notable exception of the Th2-shifting cytokine IL-19. No relevant difference was observed in cytokines regulating T cell immunity. Several growth factors having a role in regulating immunity, such as G-CSF, GM-CSF, EGF, and VEGF, were upregulated in centenarians, too. Principal component analysis of the cytokine dataset showed that pro and anti-inflammatory cytokines were the variables that contributed the most to the variability of the data we observed.https://www.mdpi.com/1422-0067/24/3/2719centenarianscytokinesPCAagingimmunosenescence |
| spellingShingle | Marcello Pinti Lara Gibellini Domenico Lo Tartaro Sara De Biasi Milena Nasi Rebecca Borella Lucia Fidanza Anita Neroni Leonarda Troiano Claudio Franceschi Andrea Cossarizza A Comprehensive Analysis of Cytokine Network in Centenarians International Journal of Molecular Sciences centenarians cytokines PCA aging immunosenescence |
| title | A Comprehensive Analysis of Cytokine Network in Centenarians |
| title_full | A Comprehensive Analysis of Cytokine Network in Centenarians |
| title_fullStr | A Comprehensive Analysis of Cytokine Network in Centenarians |
| title_full_unstemmed | A Comprehensive Analysis of Cytokine Network in Centenarians |
| title_short | A Comprehensive Analysis of Cytokine Network in Centenarians |
| title_sort | comprehensive analysis of cytokine network in centenarians |
| topic | centenarians cytokines PCA aging immunosenescence |
| url | https://www.mdpi.com/1422-0067/24/3/2719 |
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