Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites
A method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS) medium enriched with IAA and BA. Numerous shoots developed properly after they had been...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2014-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/746 |
| _version_ | 1818314428098019328 |
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| author | Barbara Thiem Lutosława Skrzypczak Eliza Lamer-Zarawska |
| author_facet | Barbara Thiem Lutosława Skrzypczak Eliza Lamer-Zarawska |
| author_sort | Barbara Thiem |
| collection | DOAJ |
| description | A method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS) medium enriched with IAA and BA. Numerous shoots developed properly after they had been transferred onto MS medium without BA. When they had rooted under the influence of auxins (IAA or IBA), shoots were transferred to pots and then to the soil, where they matured. In the seeds produced by these plants, the content of fatty acids was determined using the GC method. A preliminary analysis of flavonoid compounds and phenolic acids was made using the 2D TLC method (fingerprinting) in microshoots and leaves of soil plants regenerated in vitro. |
| first_indexed | 2024-12-13T08:49:29Z |
| format | Article |
| id | doaj.art-b59136d5b06b4957a6ed35792b56f4fc |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-13T08:49:29Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-b59136d5b06b4957a6ed35792b56f4fc2022-12-21T23:53:24ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01681152010.5586/asbp.1999.003531Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolitesBarbara Thiem0Lutosława Skrzypczak1Eliza Lamer-Zarawska2Poznań University of Medical SciencesPoznań University of Medical SciencesMedical Academy of WrocławA method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS) medium enriched with IAA and BA. Numerous shoots developed properly after they had been transferred onto MS medium without BA. When they had rooted under the influence of auxins (IAA or IBA), shoots were transferred to pots and then to the soil, where they matured. In the seeds produced by these plants, the content of fatty acids was determined using the GC method. A preliminary analysis of flavonoid compounds and phenolic acids was made using the 2D TLC method (fingerprinting) in microshoots and leaves of soil plants regenerated in vitro.https://pbsociety.org.pl/journals/index.php/asbp/article/view/746Oenothera sp.micropropagationshoot tipsnodal segments of shootscallusfatty acidsphenolic compounds |
| spellingShingle | Barbara Thiem Lutosława Skrzypczak Eliza Lamer-Zarawska Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites Acta Societatis Botanicorum Poloniae Oenothera sp. micropropagation shoot tips nodal segments of shoots callus fatty acids phenolic compounds |
| title | Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites |
| title_full | Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites |
| title_fullStr | Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites |
| title_full_unstemmed | Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites |
| title_short | Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites |
| title_sort | micropropagation of selected oenothera species and preliminary studies on their secondary metabolites |
| topic | Oenothera sp. micropropagation shoot tips nodal segments of shoots callus fatty acids phenolic compounds |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/746 |
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