Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications
In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/5/1355 |
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| author | Giuseppe Cirillo Elvira Pantuso Manuela Curcio Orazio Vittorio Antonella Leggio Francesca Iemma Giovanni De Filpo Fiore Pasquale Nicoletta |
| author_facet | Giuseppe Cirillo Elvira Pantuso Manuela Curcio Orazio Vittorio Antonella Leggio Francesca Iemma Giovanni De Filpo Fiore Pasquale Nicoletta |
| author_sort | Giuseppe Cirillo |
| collection | DOAJ |
| description | In this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found to be well tolerated by human fibroblast lung cells (MRC-5) (viability higher than 94%) and able to modify its swelling properties upon application of an external electric field. Release experiments performed using lysozyme as the model drug, showed a pH and electro-responsive behavior, with higher release amounts and rated in physiological vs. acidic pH. Finally, the retainment of the antioxidant properties of caffeic acid upon conjugation and polymerization processes (Trolox equivalent antioxidant capacity values of 1.77 and 1.48, respectively) was used to quench the effect of hydrogen peroxide in a hydrogel-assisted lysozyme crystallization procedure. |
| first_indexed | 2024-03-09T05:41:29Z |
| format | Article |
| id | doaj.art-b5930e82f92d44e6910cbda11dca8ef8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T05:41:29Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b5930e82f92d44e6910cbda11dca8ef82023-12-03T12:24:53ZengMDPI AGMolecules1420-30492021-03-01265135510.3390/molecules26051355Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical ApplicationsGiuseppe Cirillo0Elvira Pantuso1Manuela Curcio2Orazio Vittorio3Antonella Leggio4Francesca Iemma5Giovanni De Filpo6Fiore Pasquale Nicoletta7Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), ItalyNational Research Council of Italy (CNR)—Institute on Membrane Technology (ITM), 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), ItalyChildren’s Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW 2031, AustraliaDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), ItalyDepartment of Chemistry and Chemical Technologies, University of Calabria, 87036 Rende (CS), ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), ItalyIn this work, we combined electrically-conductive graphene oxide and a sodium alginate-caffeic acid conjugate, acting as a functional element, in an acrylate hydrogel network to obtain multifunctional materials designed to perform multiple tasks in biomedical research. The hybrid material was found to be well tolerated by human fibroblast lung cells (MRC-5) (viability higher than 94%) and able to modify its swelling properties upon application of an external electric field. Release experiments performed using lysozyme as the model drug, showed a pH and electro-responsive behavior, with higher release amounts and rated in physiological vs. acidic pH. Finally, the retainment of the antioxidant properties of caffeic acid upon conjugation and polymerization processes (Trolox equivalent antioxidant capacity values of 1.77 and 1.48, respectively) was used to quench the effect of hydrogen peroxide in a hydrogel-assisted lysozyme crystallization procedure.https://www.mdpi.com/1420-3049/26/5/1355hybrid hydrogelscontrolled drug deliveryprotein crystallizationlysozyme |
| spellingShingle | Giuseppe Cirillo Elvira Pantuso Manuela Curcio Orazio Vittorio Antonella Leggio Francesca Iemma Giovanni De Filpo Fiore Pasquale Nicoletta Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications Molecules hybrid hydrogels controlled drug delivery protein crystallization lysozyme |
| title | Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications |
| title_full | Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications |
| title_fullStr | Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications |
| title_full_unstemmed | Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications |
| title_short | Alginate Bioconjugate and Graphene Oxide in Multifunctional Hydrogels for Versatile Biomedical Applications |
| title_sort | alginate bioconjugate and graphene oxide in multifunctional hydrogels for versatile biomedical applications |
| topic | hybrid hydrogels controlled drug delivery protein crystallization lysozyme |
| url | https://www.mdpi.com/1420-3049/26/5/1355 |
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