State-of-the-art and novel approaches to mild solubilization of inclusion bodies
Throughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from undesired by-products towards a targeted production strategy for recombinant proteins. Inclusion bodies can easily be separated from the crude extract after cell lysis and contain the product in high purity. How...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1249196/full |
| _version_ | 1797265135504457728 |
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| author | Robert Klausser Robert Klausser Julian Kopp Julian Kopp Eva Prada Brichtova Eva Prada Brichtova Florian Gisperg Florian Gisperg Mohamed Elshazly Mohamed Elshazly Oliver Spadiut Oliver Spadiut |
| author_facet | Robert Klausser Robert Klausser Julian Kopp Julian Kopp Eva Prada Brichtova Eva Prada Brichtova Florian Gisperg Florian Gisperg Mohamed Elshazly Mohamed Elshazly Oliver Spadiut Oliver Spadiut |
| author_sort | Robert Klausser |
| collection | DOAJ |
| description | Throughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from undesired by-products towards a targeted production strategy for recombinant proteins. Inclusion bodies can easily be separated from the crude extract after cell lysis and contain the product in high purity. However, additional solubilization and refolding steps are required in the processing of IBs to recover the native protein. These unit operations remain a highly empirical field of research in which processes are developed on a case-by-case basis using elaborate screening strategies. It has been shown that a reduction in denaturant concentration during protein solubilization can increase the subsequent refolding yield due to the preservation of correctly folded protein structures. Therefore, many novel solubilization techniques have been developed in the pursuit of mild solubilization conditions that avoid total protein denaturation. In this respect, ionic liquids have been investigated as promising agents, being able to solubilize amyloid-like aggregates and stabilize correctly folded protein structures at the same time. This review briefly summarizes the state-of-the-art of mild solubilization of IBs and highlights some challenges that prevent these novel techniques from being yet adopted in industry. We suggest mechanistic models based on the thermodynamics of protein unfolding with the aid of molecular dynamics simulations as a possible approach to solve these challenges in the future. |
| first_indexed | 2024-03-12T22:47:27Z |
| format | Article |
| id | doaj.art-b5951335e5a241109838bfc30067f375 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-25T00:39:59Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b5951335e5a241109838bfc30067f3752024-03-12T12:19:07ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-07-011110.3389/fbioe.2023.12491961249196State-of-the-art and novel approaches to mild solubilization of inclusion bodiesRobert Klausser0Robert Klausser1Julian Kopp2Julian Kopp3Eva Prada Brichtova4Eva Prada Brichtova5Florian Gisperg6Florian Gisperg7Mohamed Elshazly8Mohamed Elshazly9Oliver Spadiut10Oliver Spadiut11Research Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaResearch Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaResearch Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaResearch Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaResearch Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaResearch Division Integrated Bioprocess Development, Institute of Chemical, Environmental and Bioscience, Vienna, AustriaChristian Doppler Laboratory IB Processing 4.0, Technische Universität Wien, Vienna, AustriaThroughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from undesired by-products towards a targeted production strategy for recombinant proteins. Inclusion bodies can easily be separated from the crude extract after cell lysis and contain the product in high purity. However, additional solubilization and refolding steps are required in the processing of IBs to recover the native protein. These unit operations remain a highly empirical field of research in which processes are developed on a case-by-case basis using elaborate screening strategies. It has been shown that a reduction in denaturant concentration during protein solubilization can increase the subsequent refolding yield due to the preservation of correctly folded protein structures. Therefore, many novel solubilization techniques have been developed in the pursuit of mild solubilization conditions that avoid total protein denaturation. In this respect, ionic liquids have been investigated as promising agents, being able to solubilize amyloid-like aggregates and stabilize correctly folded protein structures at the same time. This review briefly summarizes the state-of-the-art of mild solubilization of IBs and highlights some challenges that prevent these novel techniques from being yet adopted in industry. We suggest mechanistic models based on the thermodynamics of protein unfolding with the aid of molecular dynamics simulations as a possible approach to solve these challenges in the future.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1249196/fullinclusion bodiesmild solubilizationionic liquidsmolecular dynamics simulationrefoldingaggregates |
| spellingShingle | Robert Klausser Robert Klausser Julian Kopp Julian Kopp Eva Prada Brichtova Eva Prada Brichtova Florian Gisperg Florian Gisperg Mohamed Elshazly Mohamed Elshazly Oliver Spadiut Oliver Spadiut State-of-the-art and novel approaches to mild solubilization of inclusion bodies Frontiers in Bioengineering and Biotechnology inclusion bodies mild solubilization ionic liquids molecular dynamics simulation refolding aggregates |
| title | State-of-the-art and novel approaches to mild solubilization of inclusion bodies |
| title_full | State-of-the-art and novel approaches to mild solubilization of inclusion bodies |
| title_fullStr | State-of-the-art and novel approaches to mild solubilization of inclusion bodies |
| title_full_unstemmed | State-of-the-art and novel approaches to mild solubilization of inclusion bodies |
| title_short | State-of-the-art and novel approaches to mild solubilization of inclusion bodies |
| title_sort | state of the art and novel approaches to mild solubilization of inclusion bodies |
| topic | inclusion bodies mild solubilization ionic liquids molecular dynamics simulation refolding aggregates |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1249196/full |
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