<i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity
<i>Staphylococcus aureus</i> and cystic fibrosis (CF) are closely interlinked. To date, however, the impact of <i>S. aureus</i> culture in CF airways on lung function and disease progression has only been elucidated to a limited degree. This analysis aims to identify bacteria...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/5/279 |
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| author | Jonas Lange Kathrin Heidenreich Katharina Higelin Kristina Dyck Vanessa Marx Christian Reichel Willem van Wamel Martijn den Reijer Dennis Görlich Barbara C. Kahl |
| author_facet | Jonas Lange Kathrin Heidenreich Katharina Higelin Kristina Dyck Vanessa Marx Christian Reichel Willem van Wamel Martijn den Reijer Dennis Görlich Barbara C. Kahl |
| author_sort | Jonas Lange |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> and cystic fibrosis (CF) are closely interlinked. To date, however, the impact of <i>S. aureus</i> culture in CF airways on lung function and disease progression has only been elucidated to a limited degree. This analysis aims to identify bacterial factors associated to clinical deterioration. Data were collected during an observational prospective multi-center study following 195 patients from 17 centers. The average follow-up time was 80 weeks. <i>S. aureus</i> isolates (n = 3180) were scanned for the presence of 25 virulence genes and <i>agr</i>-types using single and multiplex PCR. The presence of specific virulence genes was not associated to clinical deterioration. For the <i>agr</i>-types 1 and 4, however, a link to the subjects’ clinical status became evident. Furthermore, a significant longitudinal decrease in the virulence gene quantity was observed. Analyses of the plasticity of the virulence genes revealed significantly increased plasticity rates in the presence of environmental stress. The results suggest that the phylogenetic background defines <i>S. aureus</i> pathogenicity rather than specific virulence genes. The longitudinal loss of virulence genes most likely reflects the adaptation process directed towards a persistent and colonizing rather than infecting lifestyle. |
| first_indexed | 2024-03-10T20:13:17Z |
| format | Article |
| id | doaj.art-b598f54cb64f4a9a914f62d71472bce2 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T20:13:17Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b598f54cb64f4a9a914f62d71472bce22023-11-19T22:45:44ZengMDPI AGToxins2072-66512020-04-0112527910.3390/toxins12050279<i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene PlasticityJonas Lange0Kathrin Heidenreich1Katharina Higelin2Kristina Dyck3Vanessa Marx4Christian Reichel5Willem van Wamel6Martijn den Reijer7Dennis Görlich8Barbara C. Kahl9Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, GermanyDepartment of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, 3015 CN Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, 3015 CN Rotterdam, The NetherlandsInstitute of Biostatistics and Clinical Research, University Hospital Münster, 48149 Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany<i>Staphylococcus aureus</i> and cystic fibrosis (CF) are closely interlinked. To date, however, the impact of <i>S. aureus</i> culture in CF airways on lung function and disease progression has only been elucidated to a limited degree. This analysis aims to identify bacterial factors associated to clinical deterioration. Data were collected during an observational prospective multi-center study following 195 patients from 17 centers. The average follow-up time was 80 weeks. <i>S. aureus</i> isolates (n = 3180) were scanned for the presence of 25 virulence genes and <i>agr</i>-types using single and multiplex PCR. The presence of specific virulence genes was not associated to clinical deterioration. For the <i>agr</i>-types 1 and 4, however, a link to the subjects’ clinical status became evident. Furthermore, a significant longitudinal decrease in the virulence gene quantity was observed. Analyses of the plasticity of the virulence genes revealed significantly increased plasticity rates in the presence of environmental stress. The results suggest that the phylogenetic background defines <i>S. aureus</i> pathogenicity rather than specific virulence genes. The longitudinal loss of virulence genes most likely reflects the adaptation process directed towards a persistent and colonizing rather than infecting lifestyle.https://www.mdpi.com/2072-6651/12/5/279<i>Staphylococcus aureus</i>cystic fibrosisvirulence genesphylogenyhorizontal gene transfer |
| spellingShingle | Jonas Lange Kathrin Heidenreich Katharina Higelin Kristina Dyck Vanessa Marx Christian Reichel Willem van Wamel Martijn den Reijer Dennis Görlich Barbara C. Kahl <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity Toxins <i>Staphylococcus aureus</i> cystic fibrosis virulence genes phylogeny horizontal gene transfer |
| title | <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity |
| title_full | <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity |
| title_fullStr | <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity |
| title_full_unstemmed | <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity |
| title_short | <i>Staphylococcus aureus</i> Pathogenicity in Cystic Fibrosis Patients—Results from an Observational Prospective Multicenter Study Concerning Virulence Genes, Phylogeny, and Gene Plasticity |
| title_sort | i staphylococcus aureus i pathogenicity in cystic fibrosis patients results from an observational prospective multicenter study concerning virulence genes phylogeny and gene plasticity |
| topic | <i>Staphylococcus aureus</i> cystic fibrosis virulence genes phylogeny horizontal gene transfer |
| url | https://www.mdpi.com/2072-6651/12/5/279 |
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