Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade
Abstract Hand, foot, and mouth disease (HFMD) caused by enteroviruses remains a public health threat, particularly in the Asia-Pacific region during the past two decades. Moreover, the introduction of multiple subgenotypes and the emergence of recombinant viruses is of epidemiological importance. Ba...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-12-01
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| Series: | Emerging Microbes and Infections |
| Online Access: | http://link.springer.com/article/10.1038/s41426-018-0215-x |
| _version_ | 1818588220683714560 |
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| author | Pirom Noisumdaeng Kantima Sangsiriwut Jarunee Prasertsopon Chompunuch Klinmalai Sunchai Payungporn Anek Mungaomklang Kulkanya Chokephaibulkit Rome Buathong Arunee Thitithanyanont Pilaipan Puthavathana |
| author_facet | Pirom Noisumdaeng Kantima Sangsiriwut Jarunee Prasertsopon Chompunuch Klinmalai Sunchai Payungporn Anek Mungaomklang Kulkanya Chokephaibulkit Rome Buathong Arunee Thitithanyanont Pilaipan Puthavathana |
| author_sort | Pirom Noisumdaeng |
| collection | DOAJ |
| description | Abstract Hand, foot, and mouth disease (HFMD) caused by enteroviruses remains a public health threat, particularly in the Asia-Pacific region during the past two decades. Moreover, the introduction of multiple subgenotypes and the emergence of recombinant viruses is of epidemiological importance. Based on either the full genome or VP1 sequences, 32 enteroviruses (30 from HFMD patients, 1 from an encephalitic patient, and 1 from an asymptomatic contact case) isolated in Thailand between 2006 and 2014 were identified as 25 enterovirus 71 (EV71) isolates (comprising 20 B5, 1 C2, 2 C4a, and 2 C4b subgenotypes) and 7 coxsackievirus A16 (CA16) isolates (comprising 6 B1a and 1 B1b subgenotypes). The EV71 subgenotype C4b was introduced into Thailand for the first time in 2006 and was replaced by subgenotype C4a strains in 2009. Phylogenetic, similarity plot and bootscan analyses of the complete viral genomes identified 12 recombinant viruses among the 32 viral isolates. Only one EV71-B5 isolate out of 20 was a recombinant virus with one region of intratypic or intertypic recombination, while all four EV71-C4 isolates were recombinant viruses having undergone double recombination, and all seven CA16 isolates were recombinant viruses. The recombination breakpoints of these recombinants are located solely within the P2 and P3 regions. Surveillance for circulating strains and subgenotype replacement are important with respect to molecular epidemiology and the selection of the upcoming EV71 vaccine. In addition, the clinical importance of recombinant viruses needs to be further explored. |
| first_indexed | 2024-12-16T09:21:18Z |
| format | Article |
| id | doaj.art-b59ac7f4a66341a3a0829e89427270a1 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-16T09:21:18Z |
| publishDate | 2018-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-b59ac7f4a66341a3a0829e89427270a12022-12-21T22:36:46ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512018-12-017111210.1038/s41426-018-0215-xComplete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decadePirom Noisumdaeng0Kantima Sangsiriwut1Jarunee Prasertsopon2Chompunuch Klinmalai3Sunchai Payungporn4Anek Mungaomklang5Kulkanya Chokephaibulkit6Rome Buathong7Arunee Thitithanyanont8Pilaipan Puthavathana9Faculty of Public Health, Thammasat University (Rangsit center)Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noiCenter for Research and Innovation, Faculty of Medical Technology, Mahidol UniversityDepartment of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol UniversityDepartment of Biochemistry, Faculty of Medicine, Chulalongkorn UniversityDebaratana Nakhon Ratchasima Hospital, Ministry of Public HealthDepartment of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok-noiBureau of Epidemiology, Department of Disease Control, Ministry of Public HealthDepartment of Microbiology, Faculty of Science, Mahidol UniversityCenter for Research and Innovation, Faculty of Medical Technology, Mahidol UniversityAbstract Hand, foot, and mouth disease (HFMD) caused by enteroviruses remains a public health threat, particularly in the Asia-Pacific region during the past two decades. Moreover, the introduction of multiple subgenotypes and the emergence of recombinant viruses is of epidemiological importance. Based on either the full genome or VP1 sequences, 32 enteroviruses (30 from HFMD patients, 1 from an encephalitic patient, and 1 from an asymptomatic contact case) isolated in Thailand between 2006 and 2014 were identified as 25 enterovirus 71 (EV71) isolates (comprising 20 B5, 1 C2, 2 C4a, and 2 C4b subgenotypes) and 7 coxsackievirus A16 (CA16) isolates (comprising 6 B1a and 1 B1b subgenotypes). The EV71 subgenotype C4b was introduced into Thailand for the first time in 2006 and was replaced by subgenotype C4a strains in 2009. Phylogenetic, similarity plot and bootscan analyses of the complete viral genomes identified 12 recombinant viruses among the 32 viral isolates. Only one EV71-B5 isolate out of 20 was a recombinant virus with one region of intratypic or intertypic recombination, while all four EV71-C4 isolates were recombinant viruses having undergone double recombination, and all seven CA16 isolates were recombinant viruses. The recombination breakpoints of these recombinants are located solely within the P2 and P3 regions. Surveillance for circulating strains and subgenotype replacement are important with respect to molecular epidemiology and the selection of the upcoming EV71 vaccine. In addition, the clinical importance of recombinant viruses needs to be further explored.http://link.springer.com/article/10.1038/s41426-018-0215-x |
| spellingShingle | Pirom Noisumdaeng Kantima Sangsiriwut Jarunee Prasertsopon Chompunuch Klinmalai Sunchai Payungporn Anek Mungaomklang Kulkanya Chokephaibulkit Rome Buathong Arunee Thitithanyanont Pilaipan Puthavathana Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade Emerging Microbes and Infections |
| title | Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade |
| title_full | Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade |
| title_fullStr | Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade |
| title_full_unstemmed | Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade |
| title_short | Complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus A16 recombinant strains into Thailand during the past decade |
| title_sort | complete genome analysis demonstrates multiple introductions of enterovirus 71 and coxsackievirus a16 recombinant strains into thailand during the past decade |
| url | http://link.springer.com/article/10.1038/s41426-018-0215-x |
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