Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells
The aging process impacts neural stem cells and causes a significant decline in neurogenesis that contributes to neuronal dysfunction leading to cognitive decline. Blueberries are rich in polyphenols and have been shown to improve cognition and memory in older humans. While our previous studies have...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6152 |
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| author | Tong Zheng Donna F. Bielinski Derek R. Fisher Jianyi Zhang Barbara Shukitt-Hale |
| author_facet | Tong Zheng Donna F. Bielinski Derek R. Fisher Jianyi Zhang Barbara Shukitt-Hale |
| author_sort | Tong Zheng |
| collection | DOAJ |
| description | The aging process impacts neural stem cells and causes a significant decline in neurogenesis that contributes to neuronal dysfunction leading to cognitive decline. Blueberries are rich in polyphenols and have been shown to improve cognition and memory in older humans. While our previous studies have shown that blueberry supplementations can increase neurogenesis in aged rodents, it is not clear whether this finding can be extrapolated to humans. We thus investigated the effects of blueberry treatments on adult hippocampal human neural progenitor cells (AHNPs) that are involved in neurogenesis and potentially in memory and other brain functions. Cultured AHNPs were treated with blueberry extract at different concentrations. Their viability, proliferation, and differentiation were evaluated with and without the presence of a cellular oxidative stressor, dopamine, and potential cellular mechanisms were also investigated. Our data showed that blueberry extract can significantly increase the viability and proliferation rates of control hippocampal AHNPs and can also reverse decreases in viability and proliferation induced by the cellular stressor dopamine. These effects may be associated with blueberry’s anti-inflammatory, antioxidant, and calcium-buffering properties. Polyphenol-rich berry extracts thus confer a neuroprotective effect on human hippocampal progenitor cells in vitro. |
| first_indexed | 2024-03-09T21:26:52Z |
| format | Article |
| id | doaj.art-b59ef14834ba4a70abd0873b1e430798 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:26:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b59ef14834ba4a70abd0873b1e4307982023-11-23T21:06:33ZengMDPI AGMolecules1420-30492022-09-012719615210.3390/molecules27196152Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor CellsTong Zheng0Donna F. Bielinski1Derek R. Fisher2Jianyi Zhang3Barbara Shukitt-Hale4Neuroscience and Aging Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USANeuroscience and Aging Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USANeuroscience and Aging Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAFriedman School of Nutrition Science and Policy, Tufts University, Boston, MA 02111, USANeuroscience and Aging Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USAThe aging process impacts neural stem cells and causes a significant decline in neurogenesis that contributes to neuronal dysfunction leading to cognitive decline. Blueberries are rich in polyphenols and have been shown to improve cognition and memory in older humans. While our previous studies have shown that blueberry supplementations can increase neurogenesis in aged rodents, it is not clear whether this finding can be extrapolated to humans. We thus investigated the effects of blueberry treatments on adult hippocampal human neural progenitor cells (AHNPs) that are involved in neurogenesis and potentially in memory and other brain functions. Cultured AHNPs were treated with blueberry extract at different concentrations. Their viability, proliferation, and differentiation were evaluated with and without the presence of a cellular oxidative stressor, dopamine, and potential cellular mechanisms were also investigated. Our data showed that blueberry extract can significantly increase the viability and proliferation rates of control hippocampal AHNPs and can also reverse decreases in viability and proliferation induced by the cellular stressor dopamine. These effects may be associated with blueberry’s anti-inflammatory, antioxidant, and calcium-buffering properties. Polyphenol-rich berry extracts thus confer a neuroprotective effect on human hippocampal progenitor cells in vitro.https://www.mdpi.com/1420-3049/27/19/6152human neural progenitor cellsblueberryneurogenesisproliferationneuroprotection |
| spellingShingle | Tong Zheng Donna F. Bielinski Derek R. Fisher Jianyi Zhang Barbara Shukitt-Hale Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells Molecules human neural progenitor cells blueberry neurogenesis proliferation neuroprotection |
| title | Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells |
| title_full | Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells |
| title_fullStr | Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells |
| title_full_unstemmed | Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells |
| title_short | Protective Effects of a Polyphenol-Rich Blueberry Extract on Adult Human Neural Progenitor Cells |
| title_sort | protective effects of a polyphenol rich blueberry extract on adult human neural progenitor cells |
| topic | human neural progenitor cells blueberry neurogenesis proliferation neuroprotection |
| url | https://www.mdpi.com/1420-3049/27/19/6152 |
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